EMAGE mouse gene expression spatial database

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EMAGE:17521

1700018C11Rik RIKEN cDNA 1700018C11 gene ( MGI:1922774)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005858_01	euxassay_005858_02	euxassay_005858_03	euxassay_005858_04

euxassay_005858_05	euxassay_005858_06	euxassay_005858_07	euxassay_005858_08	euxassay_005858_09

euxassay_005858_10	euxassay_005858_11	euxassay_005858_12	euxassay_005858_13	euxassay_005858_14

euxassay_005858_15	euxassay_005858_16	euxassay_005858_17	euxassay_005858_18	euxassay_005858_19

euxassay_005858_20	euxassay_005858_21	euxassay_005858_22	euxassay_005858_23	euxassay_005858_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17521_wholemount_strong.wlz

17521_wholemount_moderate.wlz

17521_wholemount_weak.wlz

17521_wholemount_possible.wlz

17521_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17521_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 06 16 17 18 19 20 21 22

humerus

weak

regional

weak expression: see section 01 02 03 21 22 23 24

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 04 22

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 04 22

hindlimb digit 4 metatarsal

weak

regional

weak expression: see section 04 22

fibula

moderate

regional

moderate expression: see section 01 weak expression: see section 22 24

tibia

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 22 23 24

femur

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 18 19 20 21 22 23

otic capsule

weak

regional

weak expression: see section 01 02 03 19 20 21 22 23

mandible

weak

regional

weak expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

axial skeleton

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13

orbito-sphenoid

weak

regional

weak expression: see section 01 02 05 06 07 08 19 20 23 24

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 01 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9767
Entity Detected:	1700018C11Rik, RIKEN cDNA 1700018C11 gene ( MGI:1922774)
Sequence:	sense strand is shown >T9767 CCAGCAACAAGTAAGTGGGGCTCAAAGGAAACAGACCTGCCTGGTGAACTGGGCTCAATTTCAACCCTGG TGATTCCACTGCAGACAAAGCTGGCAGAGTAAAACCTTCATTTCTTCTCTATGGCGGCCCACCAAACCCT TGCCTCAAAGATCTTCAGTTTATGCTGCTGGGACTGTGAGGAACCTGTTATTGAGAGCGCCAAGGTTCCA AGCCAAGAGAGCCAGACACCAACCTACAATTCGCTGAAGACTGAGAAGAGTGAGTTATGCGAACAAAACC TCAAGAGAGCTACCGCACAGTCCGTCGTTGGCCAAGAAAAGCACTCCACTGCCAGCAGTGATTATGAAGA GCTGACTGCATACAATATACAAACAGGATACCCTAAAAGGAATCTAAACCGCTACTACCAGGAACACTGG GCCCTCCGACCGTGTCTCATCGGGAGACCCTGAGGGTTCTAGTGGCAGTTCCACACAGGCTCTGAGCTGC TCAGCTACTGGAGGGTCTCCATGAGATGAAGAAGGCAGTGGACAGGAGTCATGAAGACCAATTCAGAAAC TCCACCACAAGGAGGGAAGAGGTCTGAGCCATCCCTGTGCCACTGGTGTGAGTCACCCCAGATCCTGACA GCCAGGGAGGGGGGCTGCCTGCAATTAAAGTCCTTGTTTGCAGCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6742642 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6742642 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17519 same embryo
	EMAGE:17518 same embryo
	EMAGE:17522 same embryo
	EMAGE:17520 same embryo
	EurExpress:euxassay_005858 same experiment
	MGI:4822625 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source