EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30852

Hormad1 ( MGI:1915231)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006250_01	euxassay_006250_02	euxassay_006250_03	euxassay_006250_04	euxassay_006250_05

euxassay_006250_06	euxassay_006250_07	euxassay_006250_08	euxassay_006250_09	euxassay_006250_10

euxassay_006250_11	euxassay_006250_12	euxassay_006250_13	euxassay_006250_14	euxassay_006250_15

euxassay_006250_16	euxassay_006250_17	euxassay_006250_18	euxassay_006250_19	euxassay_006250_20

euxassay_006250_21	euxassay_006250_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

ovary

moderate

regional

moderate expression: see section 01 02 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9706
Entity Detected:	Hormad1, ( MGI:1915231)
Sequence:	sense strand is shown >T9706 CACTTCAAGACTCTTGCCTGTCGCTGCCGGACGCTTCCCTCAGGCGGTGCGTAAAAAAATATTTCTTGGA AGATGGCCACTATGCAGTTGCAGAGGACAGCTTCCCTGAGTGCATTGGTATTTCCCAATAAGATATCAAC TGAGCATCAATCTTTGATGTTTGTGAAGAGGCTCCTAGCTGTTTCAGTATCTTGCATCACCTATTTGAGA GGAATATTTCCAGAACGTGCTTATGGGACAAGATATCTGGATGATCTCTGTGTCAAAATTCTGAAAGAAG ATAAAAATTGTCCAGGTTCTTCACAGCTAGTGAAGTGGATGCTTGGATGCTATGATGCTTTACAGAAGAA ATATCTAAGGATGATCATTCTAGCTGTATACACCAATCCAGGAGATCCTCAGACAATTTCAGAATGTTAC CAGTTTAAATTCAAGTACACCAAAAATGGACCAATCATGGACTTTATAAGCAAAAATCAAAACAATAAAT CTAGTACAACATCTGCTGACACCAAGAAAGCAAGTATTCTCCTCATTCGGAAGATTTATGTCTTAATGCA AAATCTAGGACCATTACCTAATGATGTTTGTCTGACCATGAAACTTTTTTACTATGATGAAGTTACACCC CCAGATTACCAACCACCAGGTTTTAAGGATGGTGACTGTGAAGGAGTAATATTTGATGGGGACCCTACAT ACTTAAATGTGGGAGAAGTCCCAACACCTTTTCACACCTTCAGATTAAAAGTGACCACTGAGAAGGAACG AATGGAAAATATTGATTCAACCATACTAAAACCAAAAGAATCAAAAACACAATTTGAAAAAATTCTAATG GACAAAGATGATGTGGAAGATGAAAATCATAATAATTTTGACATTAAAACTAAAATGAACGAACAGAATG AAAACTCTGGAGCTTCTGAAATCAAAGAACCAAATTTAGATTGTAAGGAAGAAGAAACTATGCAATTCAA AAAGAGCCAAAGTCCTTCAATTTCTCATTGTCAGGTTGAACAGTTAGTCAGTAAAACATCTGAACTTGAT GTGTCTGAAAGCAAAACAAGAAGCGGAAAAATCTTTCAGAGTAAAATGGTAAATGGAAATAATCAACAAG GACAAACTTCTAAAGAAAATCGGAAGAGAAGTCTTCGTCAATTTAGGAAAACAATAAATGCACCTGAGTG TAGGTGACAAACAAGATGCCCTGGACTTGGAGTTAAAGGTCCTTCACGTCTTGGAATCTAGTCAAGAGTC AGTGCTGAAGAAAAGGAGAGTTAGTGAACCAAAGGAACATACCTAAAAATTATTTTTCTTATGCAAATTT GCAGTTCTCAACATTTAAACTAATATGCTGTATTATTTGAAGGTGTGCCTTCTGTACCTCTGAAAATTAT TTTGTAGTTCATAGTTATTAAACTAATAAAAGTTTTAGCTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:6706580 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6706580 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006250 same experiment
	EMAGE:29258 same embryo
	EMAGE:29247 same embryo
	EMAGE:30841 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source