EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3905

Prkd1 protein kinase D1 ( MGI:99879)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 1D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.09.006] Gene Expr Patterns 6: 400-8, Oster H; Abraham D; Leitges M, Expression of the protein kinase D (PKD) family during mouse embryogenesis. Copyright 2006.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: Ba, branchial arches; Flb, forelimb bud; He, heart; Np, nasal process; Oc, otocyst.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3905_wholemount_strong_3D_1.wlz

3905_wholemount_moderate_3D_1.wlz

3905_wholemount_weak_3D_1.wlz

3905_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3905_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Broad distribution throughout the embryo.

1st branchial arch

strong

2nd branchial arch

strong

limb

strong

otocyst

strong

brain

strong

heart

detected

nasal process

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3620200
Entity Detected:	Prkd1, protein kinase D1 ( MGI:99879)
Sequence:	sense strand is shown >MGI:3620200 CTCCAATGTGTCCCTCACTGGACTCGGTACTGTCCGCACAGCATCGGCTGAGTTCTCCACCAGTGTCCCT GATGAGCCTTTACTGTCTCCTGTGAGCCCTGGCTTTGAGCAAAAGTCTCCATCTGAGTCATTTATCGGCC GTGAGAAGAGGTCAAATTCGCAGTCATACATCGGACGGCCGATTCAGCTCGACAAGCTCCTGATGTCTAA GGTGAAGGTGCCACACACCTTTGTCATCCACTCCTACACACGGCCCACGGTCTGCCAGTTCTGTAAGAAG CTCCTCAAGGGCCTCTTCCGGCAGGGCTTGCAGTGCAAAGATTGCAGATTCAACTGTCACAAACGCTGCG CACCAAAAGTACCAAACAACTGCTTGGGTGAAGTGACCATCAATGGAGAATTACTTAGCCCTGGTGCAGA ATCTGATGTTGTTATGGAAGAAGGGAGTGATGACAATGACAGTGAGCGGAACAGTGGGCTCATGGATGAC ATGGACGAGGCCATGGTCCAAGATACTGAGATGGCTTTGGCAGAGGGCCAGAGTGGAGGTGCAGAGATGC AAGATCCAGATGCAGACC
	nt 856 - nt 1433 of NM_008858.3
Notes:	The PKD1 (Prkcm) probe used in this study by Oster et al., 2006 [PMID:16377259] is described by the authors as covering the "C1 and C1/PH regions", relating to "GenBank accession number NM_008857", and being PCR amplified using "5'-CTCCAATGTGTCCCTCACTGGA-3' and 5'-GGTCTGCATCTGGATCTTGCAT-3'" from either "whole brain or E14.5 embryo". Editors note: It appears there is a typographical error in the authors description. The Seq ID should be NM_008858 (Prkcm) and not NM_008857 (Prkci) as indicated in the text. (The primers given match NM_008858 and not NM_008857, and NM_008858 is the sequence for PKD1 (Prkcm).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + Purple substrate

General Information

Authors:	Oster H; Abraham D; Leitges M, 2006 [PMID:16377259] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2005.09.006] [ PMID:16377259] Oster H, Abraham D, Leitges M 2006 Expression of the protein kinase D (PKD) family during mouse embryogenesis. Gene Expr Patterns (6):400-8
Links:	MGI:3620631 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source