EMAGE mouse gene expression spatial database

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EMAGE:10182

Slc7a8 solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 ( MGI:1355323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008218. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008218_01	euxassay_008218_02	euxassay_008218_03	euxassay_008218_04

euxassay_008218_05	euxassay_008218_06	euxassay_008218_07	euxassay_008218_08	euxassay_008218_09

euxassay_008218_10	euxassay_008218_11	euxassay_008218_12	euxassay_008218_13	euxassay_008218_14

euxassay_008218_15	euxassay_008218_16	euxassay_008218_17	euxassay_008218_18	euxassay_008218_19

euxassay_008218_20	euxassay_008218_21	euxassay_008218_22	euxassay_008218_23	euxassay_008218_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10182_wholemount_strong.wlz

10182_wholemount_moderate.wlz

10182_wholemount_weak.wlz

10182_wholemount_possible.wlz

10182_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10182_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

urinary system

strong

spotted

strong expression: see section 06 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2202
Entity Detected:	Slc7a8, solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 ( MGI:1355323)
Sequence:	sense strand is shown >T2202 TGGCCTCGAGGCCAGATTCGGATCCACTGTGGCTTCTTCTCAGGGAACCAAAAGGCAGAGGGTACACTTC TCTTTGCCTTCTTCTCCACTCTGTACTACCAAGACTCCACTGCCCAGCCTCAACCCACTACCCAGAGAGT TCCCTTTCAGGAAGCCTCCACCCCACTGAGCTAAGGACCAAGAAGGAGGACTGCCCCACCCCCAGCTCTA AGCCAAGCTTGTGGAATATACAGGGAACGCTCAGACTGCAGAAGCCAAGCCCTGCCCCACCCCTGCCGCT GCCTCCTATCCCCTTGTGGTGCCAAAGCTCCCTGAAGCCGGAAAGGCTTCTATTTAGTCTAATAGACATC TCTCTCTCAAGGGCAAAGTGACTCTGCCCCTCCCTGTCCCCCTGTGAAAGAGCAGTCACCCTCTTATCCC AGCAGCTCTTCCCACCCACAGCTCTGCCTACCACCCTCACCCCTTTCCTCTCCTGGACCACGGCTCAAGG ACCAGGTATGAAGGATCTCCAAGTCCTTCAGGCCTGAATTAGCATCAAACCAGCCTTAAGTC
Notes:	The probe template was PCR amplified from IMAGE:920332 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:920332 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10185 same embryo
	EMAGE:10184 same embryo
	EMAGE:10183 same embryo
	EurExpress:euxassay_008218 same experiment
	MGI:4828281 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source