EMAGE mouse gene expression spatial database

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EMAGE:10402

Ptpro protein tyrosine phosphatase, receptor type, O ( MGI:1097152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000528. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000528_01	euxassay_000528_02	euxassay_000528_03	euxassay_000528_04

euxassay_000528_05	euxassay_000528_06	euxassay_000528_07	euxassay_000528_08	euxassay_000528_09

euxassay_000528_10	euxassay_000528_11	euxassay_000528_12	euxassay_000528_13	euxassay_000528_14

euxassay_000528_15	euxassay_000528_16	euxassay_000528_17	euxassay_000528_18	euxassay_000528_19

euxassay_000528_20	euxassay_000528_21	euxassay_000528_22	euxassay_000528_23	euxassay_000528_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10402_wholemount_strong.wlz

10402_wholemount_moderate.wlz

10402_wholemount_weak.wlz

10402_wholemount_possible.wlz

10402_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10402_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus

moderate

regional

moderate expression: see section 05 09 13 14 15

cerebral cortex

strong

regional

strong expression: see section 19 20 21 22 23 24 weak expression: see section 01

cerebral cortex marginal layer

strong

regional

strong expression: see section 04 05 06 07 16 17 18 19

corpus striatum

strong

regional

strong expression: see section 22 weak expression: see section 20

telencephalon marginal layer

strong

regional

strong expression: see section 03

olfactory cortex

strong

regional

strong expression: see section 19 20 21

olfactory cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 16 17 18

metencephalon lateral wall

moderate

regional

moderate expression: see section 09

midbrain lateral wall

strong

spotted

strong expression: see section 04 05 06 07 13 14 15 moderate expression: see section 09

testis

strong

spotted

strong expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2303
Entity Detected:	Ptpro, protein tyrosine phosphatase, receptor type, O ( MGI:1097152)
Sequence:	sense strand is shown >T2303 GGCCTCGAGCCAGATTCGTCGACATGTCAGCCAGCTTATCTTTTCCTATTTCAAATATGAAAACCTGTGT TTCAAAGTAGAGTAGGAAAAACATTATATGTCTGACTTGTCATAGTGGGTTTCTTCCTTGTTGACAGTTG GGAGTTTCTTCCGTGGCTCTTTTGAACTAATGTCACAGTCCTTTTTGAACAGCCAGAGTTGATTCAACAG TTTTGAGTCTGACTCTGAACTCTGAGATATCTTGGTGCCTAGCCTTCACGTTTATTTTTCTCTGTGTCCA CCTGTGTACACAAAAACAGTTTCTCCAAGAGCTATAGGCTGTAAAAATGCACTTTCTTTCCCCACCCAGA GGAGCTGGGAATTCAGAAGTCACGACAACAACAAACTCAGCATGTAAGGACAGGTATTGGACACCATCCC TTAAGAACATTGTTGAGCGTCTCTATGGATTCCGACAGGAGATTCTCTGGGTCTTCTTTGCATTTCTGTT GTCAGAGTCATTTTAACCTGTGTAGCTAGTGGCATTATATTCTTTGGATTTTGTATGATTAAAGTACATG ATTGTGTGTT
Notes:	The probe template was PCR amplified from IMAGE:1137535 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1137535 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827531 same experiment
	EurExpress:euxassay_000528 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source