EMAGE mouse gene expression spatial database

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EMAGE:10957

Slc22a12 solute carrier family 22 (organic anion/cation transporter), member 12 ( MGI:1195269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003608. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003608_01	euxassay_003608_02	euxassay_003608_03	euxassay_003608_04

euxassay_003608_05	euxassay_003608_06	euxassay_003608_07	euxassay_003608_08	euxassay_003608_09

euxassay_003608_10	euxassay_003608_11	euxassay_003608_12	euxassay_003608_13	euxassay_003608_14

euxassay_003608_15	euxassay_003608_16	euxassay_003608_17	euxassay_003608_18	euxassay_003608_19

euxassay_003608_20	euxassay_003608_21	euxassay_003608_22	euxassay_003608_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10957_wholemount_strong.wlz

10957_wholemount_moderate.wlz

10957_wholemount_weak.wlz

10957_wholemount_possible.wlz

10957_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10957_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon marginal layer

moderate

regional

moderate expression: see section 05 06 07 08 18 19 20 21 22 weak expression: see section 03 04 09 10 11 12 15 16 17 23

facial vii ganglion

weak

regional

weak expression: see section 22 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 09 21

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 10 20

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 08 09 19 20 21

dorsal root ganglion

weak

regional

weak expression: see section 11 12 13 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6297
Entity Detected:	Slc22a12, solute carrier family 22 (organic anion/cation transporter), member 12 ( MGI:1195269)
Sequence:	sense strand is shown >T6297 AGCCTTGACTGGCTGTGACTCAGCAACATGGCTGTGTGACATGCATATGAAGAGACTGGTGTGGTCAGAG CCAAGGGGAAAGCAGGCTCATCACAAAGACCCCATGGTCCTGGGCCAGCCTCTGTGAAGTGGAAGCTGCG TGGTGGTACCCAGGAGAAACCCATGGCCTTTCCTGAACTCCTGGACCGAGTGGGGGGGCTGGGCAGGTTC CAGCTCTTCCAGACGGTGGCTCTGGTGACCCCCATTTTGTGGGTCACCACCCAGAACATGCTGGAGAACT TCTCAGCCGCAGTTCCCCACCACCGCTGTTGGGTGCCTCTCCTGGACAATAGCACCTCCCAGGCGAGTAT CCCTGGGGACCTTGGACCCGATGTTCTTCTGGCCGTCTCCATCCCACCTGGTCCTGACCAGCAACCCCAC CAGTGCCTCCGCTTCCGACAACCTCAATGGCAGCTTACAGAGTCCAACGCCACGGCCACCAACTGGAGTG ACGCTGCCACTGAGCCATGCGAGGATGGCTGGGTTTACGACCACAGCACCTTCAGGTCCACAATCGTGAC TATAGTGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGATTACCACAGAGGGTTCC; Reverse Primer - name:unspecified, sequence:TCACGATTGTGGACCTGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10955 same embryo
	EMAGE:10953 same embryo
	EMAGE:10956 same embryo
	EMAGE:10952 same embryo
	EMAGE:10954 same embryo
	EurExpress:euxassay_003608 same experiment
	MGI:4828131 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source