EMAGE mouse gene expression spatial database

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EMAGE:11113

Gng7 guanine nucleotide binding protein (G protein), gamma 7 ( MGI:95787)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001082. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001082_01	euxassay_001082_02	euxassay_001082_03	euxassay_001082_04

euxassay_001082_05	euxassay_001082_06	euxassay_001082_07	euxassay_001082_08	euxassay_001082_09

euxassay_001082_10	euxassay_001082_11	euxassay_001082_12	euxassay_001082_13	euxassay_001082_14

euxassay_001082_15	euxassay_001082_16	euxassay_001082_17	euxassay_001082_18	euxassay_001082_19

euxassay_001082_20	euxassay_001082_21	euxassay_001082_22	euxassay_001082_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11113_wholemount_strong.wlz

11113_wholemount_moderate.wlz

11113_wholemount_weak.wlz

11113_wholemount_possible.wlz

11113_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11113_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 04 weak expression: see section 03 05 06 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4255
Entity Detected:	Gng7, guanine nucleotide binding protein (G protein), gamma 7 ( MGI:95787)
Sequence:	sense strand is shown >T4255 GTGAAGCCTTTCTATTGGAACCTCAGTGCTGGGACGGTGGGACGGTGCAGCTCTCTAGACGCCAGGTCCA CGCTCATGGCCGATGATGTCAGGTACTAACAACGTCGCCCAGGCCCGGAAGCTGGTGGAGCAGCTGCGCA TTGAAGCTGGGATCGAACGCATCAAGGTCTCCAAGGCCTCGTCAGACCTGATGGGCTACTGTGAGCAACA TGCCCGCAACGATCCCTTGCTGGTTGGCGTGCCAGCCTCTGAGAATCCATTCAAAGACAAAAAGCCTTGC ATAATTCTCTAGTTCTCTCTCTTTCTCTCTCTCTTCCCCATCTCTCCCTCTCTTTCTCCCCACCCCGGAG GTATTGGTCCAATTTTTCCTGTAAGGAAAATATTTGAATCCTTCCCACTTTGAATTCTAAAGGAACAAAA ACCATTGTGAATGCCGGGATCTTCCTGACCTAGCTGGAGTTTATGAACCCAGGTGGGGCTCTGACTGTCG CCTGGCCTAACTGAGATGTTAGCCTCGTCACCCCCACCCCCTCTTTTCAAGAGTCACTACTGTGGCCCAT TTTTAAAGCTCCCCTTAGTTGCCTTGGGGAAAGGGCTCCGTTGATAAACGGGATTGCCTTGCAAGCGTGA GGACCCTAGGTGCGGCGCGGTCCTCAGGTCCCACAGAAAGCATGCAGGTGCAGTGTCACAAGGTTGTATG TACTTCAGCACTTGGTAGGCAAGGCAGAAGGGTCCCCAGGGCTTGAGGGACTGCCATTCTAGTTGTCAGT AAACTCCAGACAGAGGAGGACACCCAGCCTTGACCTCTGGCTCCACCCGCGCGGGGCACACACAATGCAC CCTGTGCTGCCCTGTGTCCTTCCAGAGTGGCCCTGTCATGTACACAGTGACGCCATTTCTCCAGAGACAT GGCCCATTCCAGAGACGGGCATCTGGGTCTCCATTCAAAGGAAGCTCTTGCTGGTATGACCGAGTGTGTA ATTTTGTCTCCTAAGCCTGAGATCCACGTGGTCATTGGTGGCTCCCCATTTTCTCCCAGTTACACAGTTA TCCAGGTAGTTGTGAGGGCAGAAATGCAGATTAGTTATCTCTCGAGGTGATCGAGGAGTGACGGGGTTCT CCGTCCCACATCGTGACTGTGGCTCCAGATCCCCCTGCCTCCTTCCTAGGACAAACTATTTTGTAACTAG CTAGAATCCAAGCCCCTCAGTGTGGGGACTGTAGCCCTGGTCTGCACTCCCAGATTGGGGGATTCTAGGC AGGGCTCCACCCAGACCACACCCCAATGAGGATTTGGGGACTCTAGGCGGGGCTCCACCCCGAACTGCGA CCTCAGCCCCTTACTGGGGCATTCTAGGTGGGGCTCTACATTGACCACTCCCCCAGCCCCTCACTGGGAG ATTGTAGTCTGGGGCTCCACCCTGACCACGCCCCTAGACCCTTGTTGGTAGATTCTCCACAGCATTCTAC CCCTGAGCCAGGCCTCTTCTCTTTCTATTTTGTGAACAGAGCCCTAGAGTCCAGGCTGGCCTGGAACAGG AGATCTTCCTGCCTCTGTCTCCAGAGTACCTACATGACGGTCTTGTGCTTGCATGCCTGGCATTGGATTG GACTGAAGAAAACATGCCCTCTGTCTGTAACAGTGACAGTGGCAGGTATAGGGAGGACACGACTTGGGTG TGCCAATGCGGTGTGCAGCCACACACAGAGGCAGCCTAAGCATTTGAAAGACCCCAGCTGCCTCTTTCTC CTGCCAGATCAGTATGGGAGCAAACCGCAGAACTGAAGCCAGTCTAGTTGTTCCCTGTATCCCTGGTGGA AATCTGCTGCCAAAAGCCTGGGCCAGGCTGATCCCTGGGCAAAGTGAAAGGGGTGAGCACAGTTAGCTCA TCTTCTAGCCAGTTAGGCATGACCTACCGCCTAGGTAAGGTGGCAGGCTCCCCCAAATCCATTTGATGGA AACAGGAAAAGAAGTCTGGGGTGTGACTAAGGGGAGGCATTGCAGGTGCTAAGAGCGCCCCCCTCCCACT TCCCCCTCCATGGGCTGGGTCCCAGAGAGCACAGACCAAGGGTCTGGCTTCTGTGCATCAGCTCCCATTA CTGGTCAGAGAGGGCCAGCAACTTTGGTCAGCCTGACTCTCAGAACCTGGCTTGAGTCAGAAAAGCCAGT TTCCCCCACCCTCAAACTACAGCCACCTTGCATTAGGCTAGGAACCAGGTCCCAGGATGTGTGTCCCCAG CTACACTGGCAAGGAGAAAGGAAAGCTGGAAATCTGGGGACCAGGGCTGCAGAGCACCCACCCAGCATGC TGGAAAGCTGGGTTCCACCCCAAGCTCGAAGTAACCAGGCACGGGCACTGGGGTAGGCAGATCAGGACTT CAGGGTCTTCCTCAGCTACATAATGGGCTCAAGGCTAACCTGGACTACATGAGACCATGTCTCAAATTCA GTGCACTCTCTCACTCTCTCACATCACGACAGCAGATGCCTGCCTGGCCAGCCTGTGTGTGGTCGGAGCA TTTTGTTTCTCTCCTCTGGGACAAAAAACTCAAATCTGCATCTCTGCCCACACTTCCAGTTCTCCCTCCC TAGGTGACTCACAGGCCAAGGACAAAGTGACTTCTTGTTCCCAAGGAAGGTAGGACGTGCTTGGCTGATG TATCCAGGATGGACCTTGGGCCCCAATAGCCCTTGTGTGCCCTCCTAACCACTGGAGCCCTGCCTCAGCT TAGAGATACTTAGGGAAACCAGAACACAACAGAGCCATCAGGAGCCAAGATTGGGGGCCCTCACCTTCAA TCCCAACACTTGGGAGACAGAGGCAAGGGGATCTCCGTGAATTCGAGGCCAGCCTCATCTACAGAGTGAG TTCCAGGACAGCCAGGGTTGTAGAGACACCGTCTAAAAACAAAATAAAACAACCAAAACTGTTAGACCTG CTGCAGCTTGAGCCACCCAGGAGGGACTTCCAACCTAAGGAGAAGGTTGGTGGGCATGGGGGGGTGTATC CTACACAGAAGCCATATACACTGGCCAGATGTCACATGGCACTTTCCATTTCCGTCCCCACTTCAGGGCT GGCTCCTCCCCCTCACGCTCCAGGGCTGGCTCCTACCCCTTCAGACTCCTCCCCCTTCCCTCTGCTTTGT GAAGCTGGTTGGTTTTTATTTCTGATTAAAAAAAAAACTTTTTCCCGTGTGCCACGCCCACTCTCCCAGT GTTACTATTGTGTGGAATGAGGTCACAGGAGAGTGACTCCTCCATAATAAAAGCCTCGATTTTCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503666 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503666 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11114 same embryo
	EMAGE:11117 same embryo
	EMAGE:11112 same embryo
	EMAGE:11116 same embryo
	EMAGE:11115 same embryo
	EurExpress:euxassay_001082 same experiment
	MGI:4825141 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source