EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11778

Optn optineurin ( MGI:1918898)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006947. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006947_01	euxassay_006947_02	euxassay_006947_03	euxassay_006947_04

euxassay_006947_05	euxassay_006947_06	euxassay_006947_07	euxassay_006947_08	euxassay_006947_09

euxassay_006947_10	euxassay_006947_11	euxassay_006947_12	euxassay_006947_13	euxassay_006947_14

euxassay_006947_15	euxassay_006947_16	euxassay_006947_17	euxassay_006947_18	euxassay_006947_19

euxassay_006947_20	euxassay_006947_21	euxassay_006947_22	euxassay_006947_23	euxassay_006947_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11778_wholemount_strong.wlz

11778_wholemount_moderate.wlz

11778_wholemount_weak.wlz

11778_wholemount_possible.wlz

11778_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11778_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9920
Entity Detected:	Optn, optineurin ( MGI:1918898)
Sequence:	sense strand is shown >T9920 AGTGACCTAAGAGGAGAGGATCCCTGTGGCCGGACCTGTTACCATGTCCCATCAACCTCTGAGCTGCCTG ACTGAGAAGGGGGACAGCCCTTGTGAGACCCCAGGAAATGGACCCTCCAATATGGTTCACCCCAGCCTGG ACACATTCACCCCTGAGGAGCTGCTGCAGCAAATGAAGGAACTCCTGGTTGAGAACCACCAGCTGAAAGA AGCCATGAAGCTAAATAATCAAGCTATGAAAGGGCGATTTGAGGAGCTGTCCGCCTGGACAGAGAAGCAG AAGGAAGAGCGCCTGTTGTTTGAGATGCAAAGCAAAGAGGTTAAGGAGCGCCTTAAGGCCCTGACTCATG AAAATGAGAGGCTGAAGGAAGAGCTTGGAAAATTCAAAGAGAAATCAGAAAAGCCATTGGAAGACCTCAC AGGTGGCTACAGGTATCCCAGAGCCTTGGAGGAGGAAGTGGAGAAGCTGAAGACCCAGGTGGAGCAGGAA GTGGAGCATCTGAAGATCCAGGTGATGCGCCTTCGGGCTGAAAAGGCAGACCTGCTGGGCATCGTCTCAG AACTGCAGCTCAAACTCAACTCCGGCGGCTCCTCGGAAGACTCCTTCGTTGAGATCAGGATGACCGAAGG AGAGACTGAAGGGGCAATGAAGGAGATGAAGAACTGCCCTACACCCACAAGAACAGACCCCATCAGCTTG AGCAACTGTACAGAGGATGCCAGGAGTTGTGCGGAGTTTGAAGAACTGACTGTGAGCCAGCTTCTGCTTT GCCTAAGGGAAGGAAACCAAAAGGTGGAGAGACTTGAAGTCGCCCTCAGAGAAGCCAAAGAAAGAATTTC AGATTTTGAAAAGAAAGCAAATGGCCATTCTTCTACTGAGAAGCAGACAGCGAGGAGAGCAGACAGAGAG AAGGAGGACAAAGGCCAAGAGAGTGTTGGAAGCGAAGTGGAAACACTGAGCATTCAAGTGACCTCTCTGT TTAAGGAGCTTCAAGAGGCACACACAAAACTCAGTGAGGCTGAGCTGATGAAGAAGAGACTTCAAGAAAA GTGTCAGGCTCTGGAGAGGAAGAACTCTGCAACACCATCAGAGCTGAATGAAAAGCAAGAGCTCGTTTAC AGTAACAAGAAGTTAGAGCTGCAGGTGGAGAGCATGCGCTCCGAAATCAAGATGGAGCAGGCCAAGACAG AGGAGGAGAAGTCCAGGTTAGCCACTCTGCAGGCAACTCACAACAAGCTCCTTCAAGAACATAATAAGGC ACTGAAAACAATTGAAGAACTAACCAAGCAACAGGCAGAAAAGGTGGACAAGATGTTGCTGCAGGAGCTC AGCGAGAAGCTGGAGCTGGCAGAGCAGGCTCTGGCATCCAAACAGCTCCAGATGGATGAGATGAAGCAGA CGCTCGCTAAGCAGGAGGAAGACCTGGAGACCATGGCCGTCCTCAGGGCTCAGATGGAGGTGTACTGCTC AGATTTTCACGCTGAGAGAGCAGCAAGAGAGAAGATTCATGAAGAAAAGGAGCAGCTGGCCTTGCAGCTC GCGATTTTGCTGAAAGAGAACAATGACATTGAAGAGGGAGGCAGTAGACAGTCCCTGATGGAAATGCAGT GCCGACACGGGGCAAGAACCAGTGACTCTGACCAGCAGACTTACCTGTTTCAAAGAGGAGCCGAGGACAG GAGCTGGCAGCACGGGCAGCAGCCTCGCAGTATTCCGATTCACTCCTGCCCCAAGTGCGGAGAGGTCCTG CCGGACATCGACACGCTTCAGATCCATGTGATGGACTGCATCATTTGAGTGTTCTCTCCAGTCCCCAAAG CTCTTGGGAGAGCATCTTCTGGACCGACAGGAAGTCACAAAGAGGAATCTAATTAGCACCCTTCCACATA AGCCAGCCAGTGTTTTTATGTTCTGCTCATGAGAGTCACTGTGAGCAGTCAGCCAGAATGGCTGCAGTGT TTTTCTAGGTAAATCTTCCCTTAGTGGGCATCTTGTTGTGTGTGTAATTCTGTGGAAGTTCCATGTGGCT CTTTCTGACTGAACACTGTGGTATCTGTTGACACCAGGACAGGTGAGAGATAGGGACAAGCTCAGGATTG GAGTCCTTCCCTCAGCTGTTCGGTGTGACATTACCCTCAGGCTCCGTACTGGAGCTGTACAGGATAGTAC AGTACATAGTTTAATTTTTAAAAGAACAGCTATTTTCTTTGCAGATGAAGTAGCAGCTTACAGATGTCTG AGACACTGAGAGCCTAACAGTGGGTAGCAGGACAGGAGGGAAGGTGTGGGAAGAGTGAGAGTGCTGGCCG ATGGCACAGCGTCTCAGGAGGGATAATCTGTACAGAATAGTGACTGTAATCATTTGTATATTAAGGCCAT ATCACCAGTTTAGTAAACACCTTGCAAACCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30314331 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30314331 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11779 same embryo
	EMAGE:11777 same embryo
	EMAGE:11780 same embryo
	EMAGE:11775 same embryo
	EMAGE:11776 same embryo
	EurExpress:euxassay_006947 same experiment
	MGI:4826979 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source