EMAGE mouse gene expression spatial database

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EMAGE:12719

Pparg peroxisome proliferator activated receptor gamma ( MGI:97747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011298. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011298_01	euxassay_011298_02	euxassay_011298_03	euxassay_011298_04

euxassay_011298_05	euxassay_011298_06	euxassay_011298_07	euxassay_011298_08	euxassay_011298_09

euxassay_011298_10	euxassay_011298_11	euxassay_011298_12	euxassay_011298_13	euxassay_011298_14

euxassay_011298_15	euxassay_011298_16	euxassay_011298_17	euxassay_011298_18	euxassay_011298_19

euxassay_011298_20	euxassay_011298_21	euxassay_011298_22	euxassay_011298_23	euxassay_011298_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12719_wholemount_strong.wlz

12719_wholemount_moderate.wlz

12719_wholemount_weak.wlz

12719_wholemount_possible.wlz

12719_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12719_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 06 07 08 21 22 23 24 moderate expression: see section 04 05

bladder

moderate

regional

Expression in the fundus region.

urethra of male

moderate

regional

moderate expression: see section 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30115
Entity Detected:	Pparg, peroxisome proliferator activated receptor gamma ( MGI:97747)
Sequence:	sense strand is shown >T30115 CCTTGCTGTGGGGATGTCTCACAATGCCATCAGGTTTGGGCGGATGCCACAGGCCGAGAAGGAGAAGCTG TTGGCGGAGATCTCCAGTGATATCGACCAGCTGAACCCAGAGTCTGCTGATCTGCGAGCCCTGGCAAAGC ATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCCAAGGCGAGGGCGATCTTGACAGGAAA GACAACGGACAAATCACCATTTGTCATCTACGACATGAATTCCTTAATGATGGGAGAAGATAAAATCAAG TTCAAACATATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGAATTTTTCAAGGGTGCCAGT TTCGATCCGTAGAAGCCGTGCAAGAGATCACAGAGTATGCCAAAAATATCCCTGGTTTCATTAACCTTGA TTTGAATGACCAAGTGACTCTGCTCAAGTATGGTGTCCATGAGATCATCTACACGATGCTGGCCTCCCTG ATGAATAAAGATGGAGTCCTCATCTCAGAGGGCCAAGGATTCATGACCAGGGAGTTCCTCAAAAGCCTGC GGAAGCCCTTTGGTGACTTTATGGAGCCTAAGTTTGAGTTTGCTGTGAAGTTCAATGCACTGGAATTAGA TGACAGTGACTTGGCTATATTTATAGCTGTCATTATTCTCAGTGGAGACCGCCCAGGCTTGCTGAACGTG AAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGGCCCTGGAACTGCAGCTCAAGCTGAATCACCCAG AGTCCTCTCAGCTGTTCGCCAAGGTGCTCCAGAAGATGACAGACCTCAGGCAGATCGTCACAGAGCACGT GCAGCTACTGCATGTGATCAAGAAGACAGAGACAGACATGAGCCTTCACCCCCTGCTCCAGGAGATCTAC AAGGACTTGTATTAGCAGGAAAGTCCCACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4191539), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17705. Forward Primer - name:017705_F_IRAV24-27_J06_Pparg, sequence:CCTTGCTGTGGGGATGTC; Reverse Primer - name:017705_R_SP6_IRAV24-27_J06_Pparg, sequence:GGGTGGGACTTTCCTGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12714 same embryo
	EMAGE:12718 same embryo
	EMAGE:12717 same embryo
	EMAGE:12716 same embryo
	EMAGE:12715 same embryo
	EurExpress:euxassay_011298 same experiment
	MGI:4827348 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source