EMAGE mouse gene expression spatial database

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EMAGE:13761

Dbx2 developing brain homeobox 2 ( MGI:107445)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019599. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019599_01	euxassay_019599_02	euxassay_019599_03	euxassay_019599_04

euxassay_019599_05	euxassay_019599_06	euxassay_019599_07	euxassay_019599_08	euxassay_019599_09

euxassay_019599_10	euxassay_019599_11	euxassay_019599_12	euxassay_019599_13	euxassay_019599_14

euxassay_019599_15	euxassay_019599_16	euxassay_019599_17	euxassay_019599_18	euxassay_019599_19

euxassay_019599_20	euxassay_019599_21	euxassay_019599_22	euxassay_019599_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13761_wholemount_strong.wlz

13761_wholemount_moderate.wlz

13761_wholemount_weak.wlz

13761_wholemount_possible.wlz

13761_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13761_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 01 02 18 19

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 01 17 18 19

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 01 17 18

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 17 18

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 01 17 18

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 14

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 weak expression: see section 15

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55030
Entity Detected:	Dbx2, developing brain homeobox 2 ( MGI:107445)
Sequence:	sense strand is shown >T55030 GACGGGAACATACTCCGTGTACATCTAAAAGAACCCTTAGCCAGTAACACTTGGATTAAAGCCAGTTAGA TTGTGCCTCAAAACTGCCTTAAACTAATACCTTGGCATGATGCCTGAGCTGTTGCCTAAGGAGAGCCACT CTACTGTTTCTTATTTAGCCCTAGCCTAAGGCTCAATGTATATATGGAATAGAACCTTCCACAAAGTGTG ATAAGACTGAAAGAAGAGCCAGGATCAGCCCCAGAGCCTGTTCTGAATATGTGTACATATTCGTGTGTCT GTGTGTTTGTATGTATATGCTCATTAAATACATCATCAGAAATTAGTTCTCTCCAAGTTTTATATGGAAC AAATTCATTTCATTTTGTATGAAGTAAAAAAAAAAAAAACCAACGAGCAAATATTCATTTTCTAATAGTG TAAAATCTGGGCTTTTGTGCTGCACAATTAATTATGCCGGTATAAATTCTCTGCTTCTGGCCTCTTTGGT GTCCACTGTCAATGAGTTTTAGCAAGCTTAGCAACCCTAGTCGAGTTAGCTGTGGTTAGACACACACTGA AGAGAGACAAGAGGAACTCAACAGGAAACTACTACTTTTGAATATAGTTATTAGTACCTCTAGCCCCCAG TTCTGAGGCAGCTGGGGCCATCAGTGAAGAAGGTCTACACTGAGGCGTTGATCGTCTTCTGGTCTCTTGT CTGGCTGGGTTTCCTAGGCCAGAGTCTTCTAAACACAATAAATGCCTGAGTGGAAGGAAGGGCTTGTCAG GCAACTGTTTGATCGTCTGCACCAACGAGTTTATAATCAGCGACCAAGATGAAGTCAAGACCCAGGGTCA CTGTTCTCAAACAGTCTACAACAGGGCTAAAAATCCCAGGGCTGAAGGGCTCTGTGGTCAATGCTGATTG CACACTACATACCATCTGAATTTCCCACCTAGCTCG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GACGGGAACATACTCCGTGT; Reverse Primer - name:unspecified, sequence:CGAGCTAGGTGGGAAATTCA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13762 same embryo
	EurExpress:euxassay_019599 same experiment
	MGI:4824241 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source