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EMAGE:14007

Aprt adenine phosphoribosyl transferase ( MGI:88061)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007
"Pseudo-wholemount" of euxassay_001689. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001689_01 euxassay_001689_02 euxassay_001689_03 euxassay_001689_04
EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007
euxassay_001689_05 euxassay_001689_06 euxassay_001689_07 euxassay_001689_08 euxassay_001689_09
EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007
euxassay_001689_10 euxassay_001689_11 euxassay_001689_12 euxassay_001689_13 euxassay_001689_14
EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007 EMAGE:14007
euxassay_001689_15 euxassay_001689_16 euxassay_001689_17 euxassay_001689_18 euxassay_001689_19
EMAGE:14007
euxassay_001689_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14007Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14007_wholemount_strong.wlz
14007_wholemount_moderate.wlz
14007_wholemount_weak.wlz
14007_wholemount_possible.wlz
14007_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14007_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
axial musculature
weak weak
regionalweak expression: see section 04 05 06 19 20
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 14 15
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 02 03 20
vault of skull
moderate moderate
regionalmoderate expression: see section 01 02 03
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 10 12 16 17 18 19 weak expression: see section 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3064
Entity Detected:Aprt, adenine phosphoribosyl transferase ( MGI:88061)
Sequence:sense strand is shown

>T3064
TGGCCTCGAGGCAGATTCGTCGACTCCCTGAGTTGAAACTGGTGGCGCGGCGCATCCGCAGCTTCCCCGA
CTTCCCAATCCCGGGCGTGCTGTTCAGGGATATCTCGCCCCTCTTGAAAGACCCGGACTCCTTCCGAGCT
TCCATCCGCCTCTTGGCCAGTCACCTGAAGTCCACGCACAGCGGCAAGATCGACTACATCGCAGGTCTAG
ACTCCAGGGGCTTCCTGTTTGGCCCTTCCCTAGCTCAGGAGCTGGGCGTGGGCTGTGTGCTCATCCGGAA
ACAGGGGAAGCTGCCGGGCCCCACTGTGTCAGCCTCCTATTCTCTGGAGTATGGGAAGGCTGAGCTGGAA
ATCCAGAAAGATGCCTTGGAACCCGGGCAGAGAGTGGTCATTGTGGATGACCTCCTGGCCACAGGAGGAA
CCATGTTTGCGGCCTGTGACCTGCTGCACCAGCTCCGGGCTGAAGTGGTGGAGTGTGTGAGCCTGGTGGA
GCTGACCTCGCTGAAGGGCAGGGAGAGGCTAGGACCTATACCATTCTTCTCTCTCC
Notes:The probe template was PCR amplified from IMAGE:1546518 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546518 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14010 same embryo
 EMAGE:14008 same embryo
 EMAGE:14009 same embryo
 EMAGE:6099 same embryo
 EurExpress:euxassay_001689 same experiment
 MGI:4823199 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS