EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14168

Pzp pregnancy zone protein ( MGI:87854)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001858_01	euxassay_001858_02	euxassay_001858_03	euxassay_001858_04

euxassay_001858_05	euxassay_001858_06	euxassay_001858_07	euxassay_001858_08	euxassay_001858_09

euxassay_001858_10	euxassay_001858_11	euxassay_001858_12	euxassay_001858_13	euxassay_001858_14

euxassay_001858_15	euxassay_001858_16	euxassay_001858_17	euxassay_001858_18	euxassay_001858_19

euxassay_001858_20	euxassay_001858_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14168_wholemount_strong.wlz

14168_wholemount_moderate.wlz

14168_wholemount_weak.wlz

14168_wholemount_possible.wlz

14168_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14168_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4955
Entity Detected:	Pzp, pregnancy zone protein ( MGI:87854)
Sequence:	sense strand is shown >T4955 GACCCACGCGTCCGCCCACGCGTCCGGCTCAGACGTTCTTCTCTGCCCTCTCCACCATGAGGAGAAACCA GCTGCCCACACCAGCTTTTCTTTTACTGTTCCTGCTTCTTCCCAGAGATGCCACCACAGCTACTGCAAAA CCACAATATGTGGTGCTGGTCCCGTCAGAGGTCTATTCAGGAATCCCTGAAAAGGCCTGTGTCAGCCTCA ACCATGTGAATGAGACTGTGATGCTCAGCTTAACTCTAGAGTATGCAATGCAGCAAACGAAGCTCCTCAC AGACCAGGCTGTGGATAAGGACTCCTTCTACTGCAGCCCCTTCACGATCTCAGGTTCACCTTTACCCTAC ACATTCATTACTGTGGAGATAAAAGGACCAACGCAGCGCTTCATAAAGAAGAAGTCAATACAAATAATAA AAGCTGAGAGCCCAGTCTTTGTCCAGACAGACAAACCCATATACAAACCAGGACAGATAGTGAAATTCCG AGTTGTTTCTGTGGACATCAGTTTTCGCCCATTGAATGAAACGTTCCCTGTCGTTTATATTGAGACTCCC AAGAGGAACCGAATTTTTCAATGGCAAAATATCCATCTGGCAGGAGGACTCCACCAGCTCTCTTTCCCAC TGTCTGTTGAGCCAGCTCTGGGTATCTACAAGGTTGTAGTGCAGAAGGACTCAGGGAAGAAAATAGAACA CTCCTTTGAGGTGAAGGAATACGTTTTACCCAAATTTGAGGTGATAATAAAAATGCAGAAGACTATGGCT TTCCTGGAAGAGGAACTTCCTATAACTGCTTGTGGCGTATACACATATGGAAAGCCTGTTCCCGGTCTGG TGACATTGAGAGTGTGCAGAAAATATTCACGATACCGCTCCACCTGCCATAACCAAAACTCAATGAGTAT CTGTGAAGAATTCAGCCAACAGGCAGATGATAAAGGATGTTTCAGACAAGTTGTAAAAACCAAAGTATTC CAGCTCAGACAAAAAGGCCACGACATGAAGATAGAGGTGGAAGCCAAAATCAAAGAGGAGGGAACAGGAA TAGAACTAACTGGTATTGGATCATGTGAAATAGCAAATGCCTTAAGCAAACTGAAATTTACTAAAGTAAA TACAAATTACAGGCCTGGGCTACCTTTCTCTGGACAGGTTCTTCTTGTTGATGAGAAGGGTAAACCAATC CCCAACAAAAACATAACTTCCGTCGTGTCTCCACTTGGGTACCTGTCCATTTTTACTACGGATGAGCATG GCTTGGCCAACATTTCCATTGACACTTCCAACTTCACAGCTCCGTTTTTGAGAGTTGTGGTCACCTACAA GCAGAACCATGTCTGCTATGATAACTGGTGGCTTGATGAATTTCACACACAGGCAGACCATTCTGCAACT CTAGTCTTTTCTCCAAGCCAGAGTTATATTCAACTTGAACTTGTTTTTGGTACTTTGGCCTGTGGGCAAA CTCAGGAGATTCGGATACACTACCTCTTGAATGAAGATATCATGAAGAATGAAAAAGACTTAACCTTTTA CTATCTGATCAAAGCAAGGGGAAGCATCTTCAACTTAGGAAGCCATGTGTTGTCGCTTGAACAAGGAAAC ATGAAAGGAGTCTTTTCCCTCCCAATTCAAGTGGAGCCAGGCATGGCTCCCGAGGCTCAGCTGCTCGTTT ATGCTATTTTACCTAATGAAGAACTTGTCGCTGATGCTCAGAATTTTGAAATCGAGAAGTGTTTTGCCAA CAAGGTAAATTTGAGTTTCCCATCAGCACAGAGCCTGCCAGCCTCTGACACCCACCTGAAGGTCAAAGCC GCGCCTCTGTCCCTCTGTGCCCTCACTGCAGTAGACCAGAGTGTGCTGCTACTGAAGCCCGAAGCCAAGC TCTCACCTCAGTCAATCTACAATTTGCTGCCAGGAAAGACTGTCCAGGGTGCCTTCTTTGGTGTCCCAGT GTACAAAGACCATGAGAACTGCATCAGCGGAGAAGACATCACTCACAATGGGATCGTGTACACGCCAAAG CACTCTCTGGGCGATAATGATGCACACAGCATTTTTCAGTCTGTAGGAATAAATATTTTTACCAACTCCA AAATCCACAAACCACGCTTTTGTCAAGAGTTTCAACACTATCCGGCAATGGGAGGAGTGGCACCTCAAGC CTTAGCTGTGGCTGCTTCAGGCCCAGGATCCAGCTTCAGAGCAATGGGCGTGCCGATGATGGGGTTGGAC TACTCTGATGAAATTAATCAGGTGGTGGAAGTAAGAGAGACAGTGCGGAAGTACTTCCCTGAGACCTGGA TCTGGGACCTGGTGCCACTGGACGTATCCGGGGACGGTGAATTGGCGGTAAAGGTCCCCGACACCATCAC TGAGTGGAAGGCCAGTGCATTCTGCTTGTCTGGAACCACTGGCCTTGGCCTCTCCTCCACCATCTCCCTT CAAGCCTTCCAGCCCTTCTTCTTGGAGCTCACTCTCCCATACTCTGTGGTGCGAGGGGAAGCCTTTACCC TCAAAGCCACCGTGCTCAATTACATGTCTCACTGCATTCAGATCCGAGTGGACCTAGAGATTTCTCCTGA TTTCCTGGCAGTCCCAGTGGGGGGCCATGAAAACTCTCATTGCATCTGTGGAAATGAAAGGAAAACCGTG TCCTGGGCTGTGACCCCAAAGTCGCTGGGGGAGGTGAACTTCACAGCTACCGCAGAAGCCTTGCAGTCTC CAGAATTGTGTGGCAATAAGTTGACAGAAGTGCCAGCCCTTGTACACAAGGACACTGTGGTGAAGTCCGT AATAGTTGAGCCTGAAGGAATTGAGAAGGAGCAAACGTACAACACACTGTTATGCCCACAAGATACTGAG TTACAAGATAATTGGTCACTGGAGCTTCCACCCAATGTGGTTGAAGGATCTGCCAGGGCTACACATTCCG TTTTGGGTGATATACTGGGCTCTGCAATGCAAAACCTCCAGAATCTTCTCCAGATGCCCTATGGCTGTGG GGAACAAAACATGGTCCTTTTTGTCCCTAACATCTATGTTCTGAACTATCTGAACGAGACACAGCAGCTG ACAGAGGCGATCAAGTCCAAAGCCATTAACTACCTCATCAGTGGGTACCAGAGGCAGCTGAACTATCAGC ACAGTGATGGCTCGTACAGCACATTCGGGAACCATGGTGGTGGCAACACTCCGGGAAATACTTGGCTCAC TGCGTTTGTGCTCAAGGCCTTTGCTCAAGCTCAGTCGCACATCTTTATAGAGAAGACACACATCACAAAT GCTTTCAACTGGCTCTCCATGAAACAAAAGGAGAATGGCTGTTTCCAACAGTCTGGATATCTGCTTAACA ACGCGATGAAGGGTGGTGTGGATGATGAAGTGACTCTCTCTGCCTACATCACCATTGCTCTGCTGGAGAT GCCCCTGCCTGTCACGCACAGTGTTGTGCGCAATGCTCTGTTTTGCCTGGAAACGGCCTGGGCCTCCATC TCACAGAGCCAGGAAAGTCATGTCTATACCAAGGCATTGCTGGCCTATGCCTTTGCCCTGGCAGGAAACA AGGCCAAGAGAAGCGAACTGCTTGAATCCCTAAACAAAGATGCTGTGAAGGAAGAGGATTCACTGCACTG GCAACGCCCTGGGGATGTTCAGAAAGTGAAGGCCTTATCTTTCTATCAACCTCGGGCCCCTTCTGCCGAA GTGGAGATGACAGCTTACGTGCTTCTCGCCTATCTAACCTCTGAGTCTTCCCGGCCCACACGGGACCTGT CTTCATCAGACCTGTCCACAGCATCCAAGATTGTGAAGTGGATCAGCAAGCAACAAAACTCCCACGGGGG CTTCTCCTCCACTCAGGATACAGTGGTGGCTCTCCAAGCCCTCTCCAAATATGGAGCTGCCACTTTTACG AGAAGTCAGAAAGAAGTGTTGGTCACCATCGAGTCTTCAGGGACCTTCTCTAAGACTTTCCATGTTAACA GTGGCAATCGCCTGCTGCTGCAGGAAGTCAGGCTGCCAGATCTGCCCGGAAATTACGTCACCAAAGGGTC AGGATCAGGATGTGTGTACCTTCAGACATCTCTAAAGTACAACATCCTCCCAGTGGCAGATGGAAAAGCA CCCTTCGCTCTGCAAGTCAACACTCTCCCACTAAACTTTGACAAGGCAGGAGATCACAGAACATTCCAGA TTCGCATCAACGTAAGCTACACTGGAGAACGACCCAGCTCCAACATGGTCATTGTTGATGTGAAGATGGT ATCAGGCTTCATACCTATGAAGCCATCCGTGAAAAAGCTCCAAGACCAGCCTAACATTCAGAGGACTGAA GTGAACACCAACCATGTTCTAATCTACATTGAAAAGCTAACCAATCAAACCCTCGGTTTCTCCTTCGCGG TGGAACAAGACATCCCAGTAAAGAACTTAAAACCAGCCCCCATAAAAGTCTATGATTATTATGAGACAGA TGAATTCACCGTTGAAGAATACAGTGCCCCTTTCAGTGATGGCTCTGAACAAGGAAATGCTTAAAAGTCG GAACCTATAGATGTCCTCAGAAGGACTCCTCCACACATGAAGAACCAAGAAGAAAGACAGCAAGATAAAT GGAAGGGAATTAAAAAACCATAACATTTATATGTTGAATAAAGTTATGACATTTACATCTGAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5134629 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5134629 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14167 same embryo
	EMAGE:14165 same embryo
	EMAGE:14166 same embryo
	EMAGE:14164 same embryo
	EurExpress:euxassay_001858 same experiment
	MGI:4827555 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source