EMAGE mouse gene expression spatial database

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EMAGE:14547

Rab6b RAB6B, member RAS oncogene family ( MGI:107283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005421. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005421_01	euxassay_005421_02	euxassay_005421_03	euxassay_005421_04

euxassay_005421_05	euxassay_005421_06	euxassay_005421_07	euxassay_005421_08	euxassay_005421_09

euxassay_005421_10	euxassay_005421_11	euxassay_005421_12	euxassay_005421_13	euxassay_005421_14

euxassay_005421_15	euxassay_005421_16	euxassay_005421_17	euxassay_005421_18	euxassay_005421_19

euxassay_005421_20	euxassay_005421_21	euxassay_005421_22	euxassay_005421_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14547_wholemount_strong.wlz

14547_wholemount_moderate.wlz

14547_wholemount_weak.wlz

14547_wholemount_possible.wlz

14547_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14547_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

homogeneous

strong expression: see section 06 07 19 20

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 08 18 19

trigeminal v ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 17 18 19 20

vagus x ganglion

strong

homogeneous

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 07 08 09 18 19

trigeminal v nerve

strong

homogeneous

strong expression: see section 09 10 16 17

spinal cord

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 16

cervical ganglion

strong

regional

strong expression: see section 09 16

thoracic ganglion

strong

regional

strong expression: see section 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 16 17 18 19

not examined

regional

not examined expression: see section 01 02 03 04 23

neural retina

strong

regional

strong expression: see section 01 02 03 04 weak expression: see section 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8763
Entity Detected:	Rab6b, RAB6B, member RAS oncogene family ( MGI:107283)
Sequence:	sense strand is shown >T8763 CGCCCTTCTCCTCTCCCCTCCCTGCTCCTTCACCGCTTCCTCCTCCTCCTCCTCCTCCTCGCCCGGCCCC AGCTGCCAGCACCATGTCCGCAGGGGGAGATTTTGGGAACCCGCTGCGAAAATTCAAGTTGGTTTTCCTA GGGGAGCAGAGCGTCGGGAAGACGTCTCTGATCACGAGGTTCATGTATGACAGCTTCGACAACACCTACC AGGCAACCATCGGGATTGACTTCTTGTCAAAAACCATGTACTTGGAAGACCGTACGGTTCGACTGCAGCT CTGGGACACTGCTGGGCAAGAGAGGTTCCGCAGCCTGATCCCCAGCTACATCCGAGACTCCACGGTGGCT GTGGTGGTATATGACATTACTAATCTCAACTCCTTCCAGCAGACTTCTAAATGGATTGATGACGTCAGGA CGGAGAGGGGCAGTGACGTGATCATCATGCTTGTGGGTAACAAGACGGATCTGGCTGATAAGAGGCAGAT AACCATCGAGGAAGGAGAACAGCGGGCCAAAGAACTGAGCGTCATGTTCATCGAGACCAGCGCGAAGACT GGCTACAACGTGAAGCAGCTCTTCCGACGTGTGGCGTCAGCTCTGCCCGGCATGGAGAATGTCCAGGAGA AGAGCAAAGAAGGGATGATTGACATCAAGCTGGACAAGCCTCAGGAGCCCCCGGCCAGCGAGGGCGGCTG CTCCTGTTAATGCACCGCCCACTCGGCTTCCTCAGCGCCACTCACTTGTTTTGCCTCCTATAGCTTCCTA ACGGGGTGGGAACTGAGTTTATCAAGACTGGAGGATCTTTCTTTTCTCTCTCTCTCTCTCTCTAGGAGTA GGGTGGGACATGGGGAGGGAGGCTGGGCATCAGGGACCACGTCACTCTTAACAGCTGTTACCTAAACAAC TATTTTTTGGTTTGGTTGTAATATATTGTACTTTATTAAGATTGCCAAAAACTGTTAAAATTTAAAAAAA AAAATTTAAATCATGTGTATACAACTTTTTGCCAGATTAGAAAAGTAGTCATTTTTATTTGAAAGAAGTG CTTTTTTGTTTGTTTGTTTTTGTATATTTGTAAACTTACAGAGAACCTCTCCACACACTTCTTCCTTCCT GTTCTCTTGGAACTGTGTGTCACCTCTATTTTCCTCCTACCCCCGCCCCCCCCAGCCCAGAAAATTAAAG CAATTAACTGAACAGTAGTAATTACATTCTAGTTCTGGGTCACATGGCCCAAACCAACCAAACCCGCACT CCACAGTCAAATCCAACTTTAATCTAACAAAACAGCCTCTGAGGGGGTATTGTCGAGTTCTTCCCTGGCA GAGTGGGACACATTGGCAGGCCCCTGAGAGGAGATGAAGGCCCGTGGACTGTAGATTCAGCAGCAGCTCC TTGGCTGCTTCCGCCTCTTCTCCTTCCCTGAAAAAAATAACCATAGAGCCAGAAAGCACAGGCAACAGTG TGTTCTCTGGGCTGACCCGGTGACAGACAGCAAGAAGTTACTGGAAGGATGGGGTGCGCTTGGCAGCTGA GGGCTGAGGCAGTGTGTAATCTACTGGCCCAACCATTGACACACATGGTTAGGCTGACTCGCCAACTCAA TGTGTGATGCTGGGTGGCTCAGGGACACTGAAGCCTAGGGCACCTGCCATCACTGCAACTACCATTGAGG AAGTGAGAGGAGTCGGCCATGACTCCGGACACTCCATGTTGTCAGACCCTTGGATACTAATCTTGCCGAA CGTTGTTCTGGAAAAGCTGATTCCTTGATGTGTCCTCACCTCTGGCGTGGTACGTTTGAAATCTTTGTAT GAATACCTTTGGGAGTGTTGGCAGCTGCACTGCACGCATCTTGGAGTCACTGTGGGGTTTCCAGAAGGCT GCTGCCCCAGGGTACTGCAACTTGCCCCCCAGCAGGCTGTCCTTGCTATTTCCACTCATTCCCCAGACTG AAGACCTTCAGAGTCTCTTAGCAGAGTGCTCTTCTCAAAGCAGAATAGCTCTTATATTCTAAACGCTCCC GGACTCACTGCCCCTGTCTGCCTTTGGGCAGGCCTGGGGCAGTGACTCTAATCAGAGCAGCCTAGCCCCA TAGCATTGGCTCTTCTTTCAGACTGCTGGTGGGCAGAGTGAATTGCCTCCACAAGGCAGAACTCCAACAA GGAGGTTGGGATGAATTATGGAATCTCTCAAAGAATGAGTTCAAAATGGCAGCCTGTGTGGCAGGGCTGA TTCTGTGAGTGGGCCCGTCATGCGACTCCATCCTGGGAGACTGTGTCAAAGCCGGTTCTGATGATGAAGA GAGGAAGCAGGACCGCCCTGGGTACCTCCTCCAGAAGGCAAGGACCAATGTACGAGCATAATCGAAACGT CACGTGTTTAGTTTTTATATGTGTGTACATAATTATACATAGAGATAGACTTGTGTATACACCCTTCGCG CGCACACATACACACACTCACATCCTCACATATACATGTGCTCAGTGTATATCCTCGTCTCTCCCGCTCA CAACTTGGAACTTACATTGTGATTCCGGTTTTTGGAAGTTCAAAAGTATGCAGTGTATTGAAATAAGAAA TAACTCAATATTGTCAAATAACTTGTGGTTGTCTTGCCTTCAATAAAATAGTGCATAGATGTGTTAAACT ACAAGCAAAAGTAGTCCACAGAACTACCTTTACCACTGCTTTTTGTACCTGTGTGCACAGGTGTGCATGC CGTCGTGGGCCAGTGTCCCCCACTGTGGACACCCCACCCATGTGGTGCTTGTTGGAAGAAAGCATACTTA GCCCGTGGTGACAGCAGCCTGGTTGGCAGTGAGGGGCTTTGCTGGTGGCCTCTGGCAGAGCCCCACTGAC AGCACTCTCTACTGGCTTTCACTGAATGAATGTAGGGTGAATTTTAACCTTCTGGACACTGAATAACCTT TCTTGTATTCCTGTAACTCGAATTAGAAAATCCGTTCACTTCTCTTGTGCACACAGTCTAAGACATCATG GCCTGCTACTAGAAACAGCAGGTACCATGGCAGATGCCATTTCTGGTCGATCTGGCCTAAGGAGCCCTGC CTTCCTAGCTTGTACACAAAACCCCCTCCTTGAGTTTAGACTCTCCCAGCCCTGCGGCCCCTGAGAATTA GGGTTCTGCCCCATTGCTCCCCCACTCCAGAGGTGCCCTGGCCTTATTTCCTCAGCCAAGCTACCCATCT CCTTCCCAAAAGGCAATCAAGGCAGCCAGGCCCCAACAGAACTAACTGTAGTGGACCTGGGCTTTCCCCC TGACCGCCACCACCATCCTACTTGTACGTAGGATCCAATCAGGTTCTCATAAAGACATCCTGAAATTATC TGTGCCCCCATCTTTGCTAAATTCAGATCAGTCAGAGGACAAAATGGAGGGTTCCTCTTAGGGCATGATC CCAATGTCGTAATTCAGTTCCTCTCTCTGGACACTGCCTGGCCTGGCAGATGGGCATGGCCCTCCTCCTT TTCTTTAAGAGACTTGAAAGAGAGTTGTCAGCAAAACTGTGAGCAGTGCAGAGATTGTGAAAGGGCAGGT AGTGTAAGTAGTATTGTTGTGGAGTCAGAGGTTAGAATGCTGGGTGGTTAGAGCCTTGCTGCTGCCAGTC ACTGCTCCCCGTTGCCATCTTTAAACATTTCGCAATATCTCAAGATCCAGGAAGGCATGGCCATGTCAGT TCAATGTCTCAGGCAACAAAGACAAGGGTTGCAAGGTGGTAGCTAGGAATATAGACGCCTGGGCCTAGTC TCTGTAACCCAGTGGCTAACCAGTGTCAGAGATCTAAGCAGGGCTGGCTTCAGCCTCCTGTAGGCAGGGC AGTTTATCCCTTTTCCATATGGATGGGGGAGAGGCTGTCAGCAAAGTGGCTGAAGAGAGCTAAGTTAGAG CTACCCAAGAGCCAGCCAGCAGGAGTCTGTGTCAAATGCTCAGGGCTCGATGCTCACTAGTTTCTAAACT ACCTCTCCCCCAAGAATGGGAGGCACCATCACCTTGCCACAGCACTGGGAGCCAAACCCTTCCTGTTTCA CGGGTGGGAGAACCAGAACAGAAAAGGTCACCTGCAAGCCAGAGAGGATCAGAACTAGTGTTGGCATTCT TGATCCGTTGCTGACCTTGCGGTGTCCAGACAGGACTGCTGTGCTAGCGTCCCTTAAGCCATCTCTACTC CATGGCTAGTACCTAAGGGTCTTCCCAGCCTGGCTAGCTAGAGAGAAGATAGGTGCATTTTGGGCTCTCA CTGTAAAGAAGAGTGGTCCCCAAATCTACTTGCATTGTCTTGTGTACACACACACACACACACACACACA CACCCCAGTAGTCAGGGTACCCTTTTAATTTTTGTTTGAAATCAACTTCTTCACTGTTTGGAAATCTTGC AGTTTCAATGTGCACACTCCTTGGACTCCAGACCCTGTCTGATTGATACTCTCTGCAGGGTATAATTATC AGGAAATCCACGCAGCGGGAGTTCAGATGGGCCCTTCAGGAGGCAGGAAGCCTCCATAGCAACGAAGGCC AGCCTGCCATTGTGATGTGTTGACTTCTCACCTCACCTGTCCTTTAAAGTGAGGAGCAGAGGGCGGCATT TCTATCTGTACTGGGCTCAGAAGGCACAGTGGCCATATTGACTTTACATTCAACTGTGTAATATGTGGAA AAGACTCAGCATGTTGGTCAAACAATAAACTGTCCTAATTCTCGAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406134 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406134 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14552 same embryo
	EMAGE:14548 same embryo
	EMAGE:14550 same embryo
	EMAGE:14551 same embryo
	EMAGE:14549 same embryo
	EurExpress:euxassay_005421 same experiment
	MGI:4827586 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source