EMAGE mouse gene expression spatial database

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EMAGE:14619

Gpm6a glycoprotein m6a ( MGI:107671)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005521. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005521_01	euxassay_005521_02	euxassay_005521_03	euxassay_005521_04

euxassay_005521_05	euxassay_005521_06	euxassay_005521_07	euxassay_005521_08	euxassay_005521_09

euxassay_005521_10	euxassay_005521_11	euxassay_005521_12	euxassay_005521_13	euxassay_005521_14

euxassay_005521_15	euxassay_005521_16	euxassay_005521_17	euxassay_005521_18	euxassay_005521_19

euxassay_005521_20	euxassay_005521_21	euxassay_005521_22	euxassay_005521_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14619_wholemount_strong.wlz

14619_wholemount_moderate.wlz

14619_wholemount_weak.wlz

14619_wholemount_possible.wlz

14619_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14619_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

pericardial cavity

moderate

regional

moderate expression: see section 01

pericardium

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

cornea epithelium

moderate

regional

moderate expression: see section 04 05 06 19 20

not examined

regional

not examined expression: see section 01 02 03 22 23

neural retina

moderate

regional

moderate expression: see section 01 02 03 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 weak expression: see section 07 17

nasal septum mesenchyme

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 08 10 11 12 16 weak expression: see section 09

upper lip

moderate

regional

moderate expression: see section 09 10 11 12 13 weak expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5395
Entity Detected:	Gpm6a, glycoprotein m6a ( MGI:107671)
Sequence:	sense strand is shown >T5395 AGGACTTGCCCAAGCCACTGCTGCTGGAGCTGCCCAGGGAAAAAAGGCACACGCTTTTTTGACATCTAAT TGAGCTTTTGACTAATACCGTGCGCCAGATTCTCCTGAAGAAAGGTAGCCATGGAAGAGAATATGGAAGA AGGACAGACACAGAAAGGGTGCTTCGAGTGCTGCATTAAATGCCTGGGAGGTATTCCCTATGCTTCTCTG ATTGCAACCATCCTGCTGTATGCAGGCGTTGCCCTGTTCTGTGGCTGTGGCCATGAAGCCCTTTCTGGAA CAGTCAACATTCTGCAGACCTACTTTGAGTTGGCAAGGACTGCTGGAGACACACTGGATGTTTTCACTAT GATTGACATCTTTAAGTATGTGATCTATGGCATTGCGGCTGCTTTCTTTGTCTATGGCATTTTACTGATG GTAGAAGGTTTCTTCACAACTGGGGCTATCAAAGATCTCTATGGAGACTTCAAAATCACCACCTGTGGCA GATGTGTGAGCGCTTGGTTTATCATGCTGACATACCTCTTCATGTTGGCCTGGCTGGGAGTCACAGCTTT CACCTCACTGCCCGTGTACATGTATTTCAATGTGTGGACCATCTGCCGGAACACCACTCTAGTGGAGGGA GCAAATCTCTGCTTGGATCTGCGTCAGTTTGGGATTGTGACAATTGGAGAGGAAAAGAAAATTTGTACTG CCTCTGAGAACTTCCTGAGGATGTGTGAATCTACTGAGCTGAATATGACCTTCCACTTGTTCATTGTGGC ACTTGCTGGAGCTGGAGCAGCAGTTATTGCTATGGTCCACTACCTGATGGTTCTGTCTGCCAACTGGGCC TATGTGAAAGATGCCTGCCGCATGCAGAAGTACGAAGACATCAAGTCAAAGGAAGAGCAGGAGCTGCACG ACATCCATTCTACTCGCTCCAAAGAGCGGCTCAATGCGTACACATAAATACGCTTCTCTGTTCCTTACCA CTCGAAGGCATTGGTGTTTCATTAAGGGCCATCCAACCATCCAACCTGTGAAGAGCAAAACGAGAATGCT ACTCATCAAAGATGCGCAGGGTGACTAATGAATCACCTGAGTATAACTCTTTGCTGTTGAGCATTCCATC TTCTAATTTGTTAAATTGGAATGACCAAATCTCCTCTACAAGCTCCTATTCAGTCTTTTTTTTTAACCTC TCAAATTGATTTAGTTCTTCTGTATGATCAAAGATATTAACATTGTCCAACGCAAATCTTTGTTTGATTT TTAATCACCTCCCACATATCACACATAACACTTTTTGCATTGTTTCTTATGTTTTGGAAGAGAAAATAGC TTTTTTTATACTTTTTAGTTTTGATTTCAGTAACTAGTTTAACTACAGGTAACCTTCAAAGGGACCGTTG TACATTATGAAAGAGAGACAGAGATGGTATCATGATGACTCCGCTTGATATGGGAATCAGTCTGGAGAGA TCAGTGGCCACACGGCTATCGGCCATGGTTAATGTTTTCATCCATCTAAGGTGCAATTTCAAAATTTCTA AGGGTAGGTTAAAAAAAAGTGCTTCTTATCTTTGTTAACATTGTATTCTTTTTTCTTGATGTTCGTAAAA GGTATTTCTCTGATTACTCATGTTTATGTTGTGAGCATGTAGAAACAGTAATGCTAATGCATGGCCAGTT GCCTTCCTAAGATTGTGACACCAGGCTTACCTTTTCAAGTTTAGTATAGAAACAATTATCATGGAAATAA CTACTGTGGACTGTTGGAGAGTGATCTTTTTTTGTGATTTATGCAGTGGCTGGATTGGATCTTTTAATAT GCTAATGTGGAAAATTAATTACCTTTATGAAGGTGGTATCTTAAACATAAGCACACTAAACCCTCAAGAC TTGTTTTACCCACTTAAAACTTTTAACGGATTGCACCTCTGTAAGCTATTTCTTAAAATGTGTATGATAT ATTTGAAAAGACTTCCATTAATATAATAGCTTTGCTTGCAGCCTTCCAATCTATGTTGGTTTACCTGTAG TGTTTCTAAAGGGTGGTCAGAGGCCACTAGAGAATGTATTGTTTGGGAGTGTAGTGATATATATTTTGTG TTTTTATTTCAAGTAAGTCATTTTAACCAAATGTTCATTCACATTCATTTATAAGAAGTACCTATATCAG ACAAATTTTAAAAGAAAAACAATCAGTATTAATGGACCAAATTTCGTGTTTGTTTTAACCTTGACTCTCT TTTGATTCTTTCTAATGCTACAAGAATGCTGTGAAATGTCATTTAAAATAATGATGTAGCCTGACAAGAA ATTTTGTCAGTGTATAAACCTAGGTAGTATTGTGCACTGATTTGACCATTGTGAATTCCTCCTTCAGTGT AAGTGCATTCCCAATAAAATTTATTGAGTGAAACAATCTTTGTACAACTACTAGTCATGCATCATCAGTA ATTTTACAAGTTCTTGTAGTAGGTAGGGGGTATTACTAGCGTTTCTGTGGCATGATTATGCATTCTGTAA TATTAATTTGGGGGTCCTTTTGCTTTTTAACACTTAGATTATCTTAATGGTTCAACATTTTTCTGATATA TGCAGTATTAAAGATATTGAGCAAAAGTATTAATGGGCTTCTTTAAATTCTATATTATAGTGTTTCAGTT CTGTGTCTACAGTGTGTGATGATTTTTAAAACTGTCTTTTAAATTTATGTAATGATGTTGACATTTTGAC TTTTATTTCTGGTTTTAGAGTTTGTATTTTCATGGAGTGCTTTGTAGCAGGCATTACAAGTAATCTGTTT TGTACATAAATGTGCCAACAGCTTGATGGTGGCGTTTTTGAAATGTAGAACAAAGTGCTTGCAAAATGTA ATAAATACACTTGTGTACTTTGTGTACTTATTCTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365175 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365175 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14616 same embryo
	EMAGE:14617 same embryo
	EMAGE:14618 same embryo
	EMAGE:14620 same embryo
	EurExpress:euxassay_005521 same experiment
	MGI:4825164 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source