EMAGE mouse gene expression spatial database

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EMAGE:14741

Npm1 nucleophosmin 1 ( MGI:106184)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005708_01	euxassay_005708_02	euxassay_005708_03	euxassay_005708_04	euxassay_005708_05

euxassay_005708_06	euxassay_005708_07	euxassay_005708_08	euxassay_005708_09	euxassay_005708_10

euxassay_005708_11	euxassay_005708_12	euxassay_005708_13	euxassay_005708_14	euxassay_005708_15

euxassay_005708_16	euxassay_005708_17	euxassay_005708_18	euxassay_005708_19	euxassay_005708_20

euxassay_005708_21	euxassay_005708_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14741_wholemount_strong.wlz

14741_wholemount_moderate.wlz

14741_wholemount_weak.wlz

14741_wholemount_possible.wlz

14741_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14741_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8845
Entity Detected:	Npm1, nucleophosmin 1 ( MGI:106184)
Sequence:	sense strand is shown >T8845 CCTGCGCGTCTGTTCTGTGGAACAGGAGGCAGTTGTTTTCCGTCCGGCTTCTCCCACACCGAAGTGCGCG CCTCCACCTCATGGAAGACTCGATGGATATGGACATGAGTCCTCTTAGGCCTCAGAACTACCTTTTCGGC TGTGAACTAAAGGCTGACAAAGACTATCACTTTAAAGTGGATAATGATGAAAATGAGCACCAGTTGTCAT TAAGAACGGTCAGTTTAGGAGCAGGGGCAAAAGATGAGTTACACATCGTAGAGGCAGAAGCAATGAACTA TGAAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAAATGTCTGTACAACCAACAGTTTCCCTAGGG GGCTTTGAAATTACACCACCTGTGGTCTTACGGTTGAAGTGTGGTTCAGGGCCTGTGCACATTAGTGGAC AGCATCTAGTAGCTGTAGAGGAAGATGCAGAGTCTGAAGATGAAGATGAGGAGGACGTAAAACTCTTAGG CATGTCTGGAAAGCGATCTGCTCCTGGAGGTGGTAACAAGGTTCCACAGAAAAAAGTAAAACTTGATGAA GATGATGAGGACGATGATGAGGACGATGAGGATGATGAGGATGATGATGATGATGATTTTGATGAAGAGG AAACTGAAGAAAAGGTCCCAGTGAAGAAATCTGTACGAGATACCCCAGCCAAAAATGCACAAAAATCAAA CCAAAATGGAAAAGACTTAAAACCATCAACACCGAGATCAAAGGGTCAAGAGTCCTTCAAAAAACAGGAA AAGACTCCTAAAACACCAAAAGGACCTAGTTCTGTAGAAGACATTAAGGCAAAAATGCAAGCAAGTATAG AAAAAGGCGGTTCTCTTCCCAAAGTGGAAGCCAAGTTCATTAATTATGTGAAGAATTGTTTCCGGATGAC TGACCAGGAGGCTATTCAAGATCTCTGGCAGTGGAGGAAATCTCTTTAAGAAAAGGGTTTAAACAGTTTG AAATATTCTGTCTTCATTTCTGTAATAGTTAATATCTGGCTGTCCTTTTTATAATGCAAAGTGAGAACTT TCCCTACTGTGTTTGATAAATGTTGTCCAGGTTCACTTGCCAAGAATGTGTTGTCTAAAATGCCTGTTTA GTTTTCAAGGATGGAACTCCACCCTTTACTTGGTTTTAAGTATGTATGGAATGTTATGATAGGACATAGT AATAGTGGTCAGATGTGGAAATGGTAGGGAGACAAATATACATGTGAAATAAACTCAGTATTTTAATAAA GTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6411700 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6411700 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14743 same embryo
	EMAGE:14742 same embryo
	EMAGE:14744 same embryo
	EMAGE:14740 same embryo
	EurExpress:euxassay_005708 same experiment
	MGI:4826765 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source