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EMAGE:15204

Sytl3 synaptotagmin-like 3 ( MGI:1933367)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204
"Pseudo-wholemount" of euxassay_010412. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010412_01 euxassay_010412_02 euxassay_010412_03 euxassay_010412_04
EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204
euxassay_010412_05 euxassay_010412_06 euxassay_010412_07 euxassay_010412_08 euxassay_010412_09
EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204
euxassay_010412_10 euxassay_010412_11 euxassay_010412_12 euxassay_010412_13 euxassay_010412_14
EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204
euxassay_010412_15 euxassay_010412_16 euxassay_010412_17 euxassay_010412_18 euxassay_010412_19
EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204 EMAGE:15204
euxassay_010412_20 euxassay_010412_21 euxassay_010412_22 euxassay_010412_23 euxassay_010412_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15204Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15204_wholemount_strong.wlz
15204_wholemount_moderate.wlz
15204_wholemount_weak.wlz
15204_wholemount_possible.wlz
15204_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15204_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 09 10 11 12 13
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30238
Entity Detected:Sytl3, synaptotagmin-like 3 ( MGI:1933367)
Sequence:sense strand is shown

>T30238
TCCCGACCCTGTTTTGAAGCAAGACACTCCCCCGTCCAGTCCGACACACAGCGCTGTCTTCTCTGGAGGC
TTGAGACACGGAAGCTTAATTAGCATCAACAGCACCTGCACAGAGATGGGTAATTTTGACAATGCTAATG
TCACTGGAGAAATAGAATTTGCCATTCATTACTGCGTTAAAAGCTGCTCTTTAGAGATATGCATCAAGAC
CTGTAAGAACCTTGCCTATGGAGAAGAAAAGAAAAGAAAGTGCAATCCGTATGTCAAGACCTACCTGCTG
CCTGACAGGTCTTCTCAGGGAAAACGCAAGACACGAGTCCAAAAGAACACGCTGGACCCGACTTTCGAGG
AAACCTTGAAGTATCAGGTGGACCCTGGCCAGCTGATGACCCGGAGGCTGCAGGTCTCTGTGTGGCACCT
AGGCACCCTTGCCCGGAGGGTGTTCCTCGGAGAAGTGATCCTTCCACTGGCCATGTGGGACTTTAAGGAC
AGTACGGCACAGAATGCCAGATGGTATCCTCTGCGGGCCAAGGCTGAGAAATACGAAGAGAATATTCCTC
AGAATAATGGAGAACTTGCTGTTCGAGCCAAATTGGTCCTTCCCGCAGGGCCAAGAAAGCCCCAGGAGGC
CCAAGAAGGTCAGCTAGCGCTTAATGGTCAACTGTGTCTGGTTGTGTTGGGAGCCAAGAATTTACCGGTG
AGGTCAGATGGCACCTTAAACTCATTTGTTAAGGGCTGTCTCACCCTGCCAAACCAACAAAAGCTGCGTG
TGAAGTCCCCAGTCCTGAAGAAGCAGGCTTGTCCCCAGTGGAAACACTCCTTTGTGTTTAATGGTGTGAG
CAGTTCTCAGCTGAGACAGTCCACCTTGGAGCTGACTGTCTGGGACCAGGCCATCTTTGGTATGAACGAC
CGTCTGCTCGGGGAAGCCAGGCTTGGTTCAAAGGGAGGTGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4166796), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26386. Forward Primer - name:026386_F_IRAV59-62_P11_Sytl3, sequence:TCCCGACCCTGTTTTGAA; Reverse Primer - name:026386_R_SP6_IRAV59-62_P11_Sytl3, sequence:GCAGCACCTCCCTTTGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15201 same embryo
 EMAGE:15199 same embryo
 EMAGE:15202 same embryo
 EMAGE:15203 same embryo
 EMAGE:15200 same embryo
 EurExpress:euxassay_010412 same experiment
 MGI:4828576 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS