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EMAGE:15407

Avpr1a arginine vasopressin receptor 1A ( MGI:1859216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407
"Pseudo-wholemount" of euxassay_010604. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010604_01 euxassay_010604_02 euxassay_010604_03 euxassay_010604_04
EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407
euxassay_010604_05 euxassay_010604_06 euxassay_010604_07 euxassay_010604_08 euxassay_010604_09
EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407
euxassay_010604_10 euxassay_010604_11 euxassay_010604_12 euxassay_010604_13 euxassay_010604_14
EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407 EMAGE:15407
euxassay_010604_15 euxassay_010604_16 euxassay_010604_17 euxassay_010604_18 euxassay_010604_19
EMAGE:15407 EMAGE:15407
euxassay_010604_20 euxassay_010604_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15407Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15407_wholemount_strong.wlz
15407_wholemount_moderate.wlz
15407_wholemount_weak.wlz
15407_wholemount_possible.wlz
15407_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15407_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lower lip
weak weak
regionalweak expression: see section 09 11 12 13 14 15
upper lip
moderate moderate
regionalmoderate expression: see section 06 07 08 19 weak expression: see section 05 09 11 12 13 15 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31154
Entity Detected:Avpr1a, arginine vasopressin receptor 1A ( MGI:1859216)
Sequence:sense strand is shown

>T31154
ATGACGGCTGACCGCTACATCGCCGTGTGCCACCCGCTCAAGACCCTGCAGCAGCCCGCGCGCCGCTCGC
GCCTCATGATCGCCGCCTCTTGGGGGCTGAGTTTCGTTCTGAGCATACCACAGTACTTTATCTTCTCTGT
GATCGAATTTGAGGTGAACAATGGCACCAAAGCCCAAGATTGCTGGGCTACCTTCATCCCGCCCTGGGGT
ACCCGTGCCTACGTGACCTGGATGACCAGCGGGGTCTTCGTGGTACCCGTGATCATCTTGGGTACCTGCT
ATGGCTTCATCTGTTACCACATCTGGCGCAATGTCCGAGGGAAGACAGCATCGCGACAGAGCAAAGGCGG
CAAGGGCTCTGGGGAAGCCGCGGGTCCCTTCCACAAGGGGCTTCTGGTCACGCCTTGTGTCAGCAGCGTG
AAGAGCATTTCCCGTGCCAAGATCCGCACAGTGAAGATGACCTTTGTGATTGTAAGCGCCTACATCCTCT
GCTGGACACCTTTCTTCATCGTCCAGATGTGGTCAGTCTGGGATACCAATTTCGTTTGGACCGATTCCGA
AAACCCTTCCACCACGATCACGGCGTTACTGGCTTCCTTGAACAGCTGCTGCAACCCGTGGATCTACATG
TTTTTTAGTGGTCATCTCCTACAAGATTGTGTCCAGAGCTTCCCATGCTGCCAAAGCATAGCGCAGAAAT
TCGCCAAGGATGACTCGGATAGCATGAGCCGGAGACAGACATCTTATTCTAACAACCGAAGCCCAACAAA
CAGCACTGGGACGTGGAAGGATTCACCTAAATCTTCAAAGTCCATCAGATTTATCCCTGTCTCCACTTGA
GCCGCATATCCACGCAGCCAGACT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4220075), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 53348. Forward Primer - name:053348_F_IRAV45_a01_Avpr1a, sequence:ATGACGGCTGACCGCTAC; Reverse Primer - name:053348_R_SP6_IRAV45_a01_Avpr1a, sequence:GAGTCTGGCTGCGTGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15410 same embryo
 EMAGE:15411 same embryo
 EMAGE:15406 same embryo
 EMAGE:15408 same embryo
 EMAGE:15409 same embryo
 EurExpress:euxassay_010604 same experiment
 MGI:4823363 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS