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EMAGE:15465

Ttr transthyretin ( MGI:98865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465
"Pseudo-wholemount" of euxassay_010654. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010654_01 euxassay_010654_02 euxassay_010654_03 euxassay_010654_04
EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465
euxassay_010654_05 euxassay_010654_06 euxassay_010654_07 euxassay_010654_08 euxassay_010654_09
EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465
euxassay_010654_10 euxassay_010654_11 euxassay_010654_12 euxassay_010654_13 euxassay_010654_14
EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465
euxassay_010654_15 euxassay_010654_16 euxassay_010654_17 euxassay_010654_18 euxassay_010654_19
EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465 EMAGE:15465
euxassay_010654_20 euxassay_010654_21 euxassay_010654_22 euxassay_010654_23 euxassay_010654_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15465Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15465_wholemount_strong.wlz
15465_wholemount_moderate.wlz
15465_wholemount_weak.wlz
15465_wholemount_possible.wlz
15465_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15465_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
pancreas
moderate moderate
regionalmoderate expression: see section 08 09 10 11 14 15 16
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
stomach
weak weak
regionalweak expression: see section 05 06 07 08 09 10 14 15 16
midgut
weak weak
regionalweak expression: see section 14 15 16 17 18 19 20
liver
strong strong
regionalstrong expression: see section 08
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31167
Entity Detected:Ttr, transthyretin ( MGI:98865)
Sequence:sense strand is shown

>T31167
GGATGGCTTCCCTTCGACTCTTCCTCCTTTGCCTCGCTGGACTGGTATTTGTGTCTGAAGCTGGCCCCGC
GGGTGCTGGAGAATCCAAATGTCCTCTGATGGTCAAAGTCCTGGATGCTGTCCGAGGCAGCCCTGCTGTA
GACGTGGCTGTAAAAGTGTTCAAAAAGACCTCTGAGGGATCCTGGGAGCCCTTTGCCTCTGGGAAGACCG
CGGAGTCTGGAGAGCTGCACGGGCTCACCACAGATGAGAAGTTTGTAGAAGGAGTGTACAGAGTAGAACT
GGACACCAAATCGTACTGGAAGACACTTGGCATTTCCCCGTTCCATGAATTCGCGGATGTGGTTTTCACA
GCCAACGACTCTGGCCATCGCCACTACACCATCGCAGCCCTGCTCAGCCCATACTCCTACAGCACCACGG
CTGTCGTCAGCAACCCCCAGAATTGAGAGACTCAGCCCAGGAGGACCAGGATCTTGCCAAAGCAGTAGCA
TCCCATTTGTACCAAAACAGGGTTTTTGTTTTATAAACCGTGTTAGCAGCTCAGGAAGATGCCGGGAAGC
ATTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4192268), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 53673. Forward Primer - name:053673_F_IRAV46_a10_Ttr, sequence:GGATGGCTTCCCTTCGAC; Reverse Primer - name:053673_R_SP6_IRAV46_a10_Ttr, sequence:AAAAATGCTTCCCGGCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15466 same embryo
 EMAGE:15467 same embryo
 EMAGE:15468 same embryo
 EMAGE:15469 same embryo
 EMAGE:15464 same embryo
 EurExpress:euxassay_010654 same experiment
 MGI:4829002 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS