EMAGE mouse gene expression spatial database

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EMAGE:15727

Tbx20 T-box 20 ( MGI:1888496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010916. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010916_01	euxassay_010916_02	euxassay_010916_03	euxassay_010916_04

euxassay_010916_05	euxassay_010916_06	euxassay_010916_07	euxassay_010916_08	euxassay_010916_09

euxassay_010916_10	euxassay_010916_11	euxassay_010916_12	euxassay_010916_13	euxassay_010916_14

euxassay_010916_15	euxassay_010916_16	euxassay_010916_17	euxassay_010916_18	euxassay_010916_19

euxassay_010916_20	euxassay_010916_21	euxassay_010916_22	euxassay_010916_23	euxassay_010916_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15727_wholemount_strong.wlz

15727_wholemount_moderate.wlz

15727_wholemount_weak.wlz

15727_wholemount_possible.wlz

15727_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15727_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 09 14 15 16

pons mantle layer

strong

regional

strong expression: see section 07 16 17 18

heart atrium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

heart valve

strong

regional

strong expression: see section 10 11 12 13 14

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

bladder

moderate

regional

moderate expression: see section 13 14 15 16

tail paraxial mesenchyme

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 moderate expression: see section 06 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38911
Entity Detected:	Tbx20, T-box 20 ( MGI:1888496)
Sequence:	sense strand is shown >T38911 CCTATACAGGGTTCTCTGCCACCATATAGCCGCCTGGGAATGCCTCTGACCCCATCTGCAATAGCCAGCT CCATGCAGGGAAGTGGTCCCACGTTCCCTTCATTCCACATGCCTAGATACCATCACTACTTCCAGCAGGG GCCCTACGCTGCCATCCAAGGACTTCGCCACTCCTCCGCTGTGATGACGCCATTTGTATGATTCCACTGA GAGTCGGGGAAGCTAGATACAGAGTGTGCAAATGGCTATGAAAATACAGGAGGGGTGGGAAGTAGGGTGG GTGGGAAGTGGGGTATTGGAGCAAGGACTCTCCAGGAAACACAGGACTTATTAAAGGAAAGAGGAGAGCT GGACTCGCAAAACACGTACTCGCAAGGGAGTGCACCACGGGCCGGGCTTCATCTGTGATTGGATCCAGGG AAGAATCAGCGTGAAGTATA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 267412. Forward Primer - name:267412_F_cDNA_Tbx20, sequence:CCTATACAGGGTTCTCTGCCAC; Reverse Primer - name:267412_N_SP6_cDNA_Tbx20, sequence:TATACTTCACGCTGATTCTTCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15729 same embryo
	EMAGE:15725 same embryo
	EMAGE:15724 same embryo
	EMAGE:15728 same embryo
	EMAGE:15726 same embryo
	EurExpress:euxassay_010916 same experiment
	MGI:4828624 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source