EMAGE mouse gene expression spatial database

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EMAGE:15809

Rtn4rl2 reticulon 4 receptor-like 2 ( MGI:2669796)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013217. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013217_01	euxassay_013217_02	euxassay_013217_03	euxassay_013217_04

euxassay_013217_05	euxassay_013217_06	euxassay_013217_07	euxassay_013217_08	euxassay_013217_09

euxassay_013217_10	euxassay_013217_11	euxassay_013217_12	euxassay_013217_13	euxassay_013217_14

euxassay_013217_15	euxassay_013217_16	euxassay_013217_17	euxassay_013217_18	euxassay_013217_19

euxassay_013217_20	euxassay_013217_21	euxassay_013217_22	euxassay_013217_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15809_wholemount_strong.wlz

15809_wholemount_moderate.wlz

15809_wholemount_weak.wlz

15809_wholemount_possible.wlz

15809_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15809_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 10 11 12 13 15 17 18 19 20 21 22 23

olfactory cortex marginal layer

weak

regional

weak expression: see section 10 11 12 15

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15

facial vii ganglion

weak

regional

weak expression: see section 03 04 05 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39061
Entity Detected:	Rtn4rl2, reticulon 4 receptor-like 2 ( MGI:2669796)
Sequence:	sense strand is shown >T39061 CTAGAAGAACTGGACCTCGGTGACAACCGGCACCTGCGCTCCCTGGAGCCCGACACCTTCCAGGGTCTGG AGAGGCTGCAGTCACTACACCTGTATCGTTGCCAGCTCAGCAGCCTGCCTGGCAACATTTTCCGAGGCTT GGTCAGCCTACAGTACCTCTACCTCCAGGAGAACAGCCTGCTCCATCTACAGGATGACTTGTTCGCGGAC CTGGCCAACCTGAGCCACCTCTTCCTCCACGGGAACCGCCTGCGGCTGCTCACGGAGCACGTGTTCCGCG GCTTGGGCAGCCTGGACCGGCTGTTGCTGCACGGGAACCGGCTGCAGGGCGTGCACCGCGCGGCTTTCCA CGGCCTCAGCCGCCTCACCATCCTCTACCTGTTCAACAACAGCCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95821. Forward Primer - name:095821_F_cDNA_Rtn4rl2, sequence:CTAGAAGAACTGGACCTCGGTG; Reverse Primer - name:095821_N_SP6_cDNA_Rtn4rl2, sequence:CCAGGCTGTTGTTGAACAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15810 same embryo
	EMAGE:15813 same embryo
	EMAGE:15812 same embryo
	EMAGE:15814 same embryo
	EMAGE:15811 same embryo
	EurExpress:euxassay_013217 same experiment
	MGI:4827832 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source