EMAGE mouse gene expression spatial database

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EMAGE:16789

Efemp1 epidermal growth factor-containing fibulin-like extracellular matrix protein 1 ( MGI:1339998)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008783. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008783_01	euxassay_008783_02	euxassay_008783_03	euxassay_008783_04

euxassay_008783_05	euxassay_008783_06	euxassay_008783_07	euxassay_008783_08	euxassay_008783_09

euxassay_008783_10	euxassay_008783_11	euxassay_008783_12	euxassay_008783_13	euxassay_008783_14

euxassay_008783_15	euxassay_008783_16	euxassay_008783_17	euxassay_008783_18	euxassay_008783_19

euxassay_008783_20	euxassay_008783_21	euxassay_008783_22	euxassay_008783_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16789_wholemount_strong.wlz

16789_wholemount_moderate.wlz

16789_wholemount_weak.wlz

16789_wholemount_possible.wlz

16789_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16789_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 11 12 15 16 17 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 04 05 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1320
Entity Detected:	Efemp1, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 ( MGI:1339998)
Sequence:	sense strand is shown >T1320 CCTCGAGCCTGTTGGCCTACTGGTACCAGGCTGCCCTCCCCTACGCACTCCTTCGCTGCCCGGGCAGGAA AGTTGCGCGGCCCCCCCGCAGAGAATCACGATGTTGCAAACACTTTTCCTAACTATGCTGACTCTGGCGC TGGTCAAGTCACAGTACACCGAGGAAACCATCACATACACGCAATGCACCGATGGATATGAATGGGACCC CATAAGACAGCAGTGTAAAGATATTGATGAATGTGACATTGTCCCAGATGCTTGCAAAGGTGGAATGAAA TGTGTCAACCACTATGGAGGATATCTCTGCCTTCCTAAAACAGCCCAAATTATTGTCAATAATGAGCATC CTCAGCAAGAAACACCAGCTGCAGAAGCTTCGTCAGGTGCCACCACTGGGACTGTAGCTGCCAGGAGCAT GGCAACCAGTGGTGTGGTGCCTGGGGGTGGCTTCATGGCCAGTGCTACTGCAGTTGCTGGCCCTGAAGTT CAAACTGGCCGAAATAACTTTGTCATCCGAAGAAACCCAGCTGACCCTCAGCGCATCCCTTCCAACCCTT C
Notes:	The probe template was PCR amplified from IMAGE:2270161 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2270161 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16790 same embryo
	EMAGE:16788 same embryo
	EMAGE:16787 same embryo
	EurExpress:euxassay_008783 same experiment
	MGI:4824480 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source