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EMAGE:17048

Adipor2 adiponectin receptor 2 ( MGI:93830)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048
"Pseudo-wholemount" of euxassay_001439. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001439_01 euxassay_001439_02 euxassay_001439_03 euxassay_001439_04
EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048
euxassay_001439_05 euxassay_001439_06 euxassay_001439_07 euxassay_001439_08 euxassay_001439_09
EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048
euxassay_001439_10 euxassay_001439_11 euxassay_001439_12 euxassay_001439_13 euxassay_001439_14
EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048 EMAGE:17048
euxassay_001439_15 euxassay_001439_16 euxassay_001439_17 euxassay_001439_18 euxassay_001439_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17048Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17048_wholemount_strong.wlz
17048_wholemount_moderate.wlz
17048_wholemount_weak.wlz
17048_wholemount_possible.wlz
17048_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17048_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 04 05 13 14
trigeminal v ganglion
weak weak
regionalweak expression: see section 02 03 04 05 06 07 13 14 15 16 17
vagus x ganglion
weak weak
regionalweak expression: see section 06 12
tail dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 08 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3230
Entity Detected:Adipor2, adiponectin receptor 2 ( MGI:93830)
Sequence:sense strand is shown

>T3230
TGGCCTCGAGCAGATTCGGACGAGGCTGATTGTCATCTGTGTGCTGGGCATTGCAGCCATTATCGTCTCT
CAGTGGGACATGTTTGCCACCCCTCAGTATCGGGGGGTCAGAGCAGGAGTGTTCGTGGGCTTAGGCCTGA
GTGGAATCATCCCTACCTTGCATTATGTCATCTCAGAAGGGTTCCTGAAGGCTGCCACCATAGGGCAGAT
AGGCTGGCTAATGCTTATGGCTAGCCTCTATATCACCGGAGCTGCCCTCTATGCGGCCCGTATCCCTGAG
CGCTTCTTTCCTGGCAAATGTGACATCTGGTTTCACTCTCATCAGCTCTTCCACATCTTTGTGGTTGCTG
GTGCCTTTGTTCACTTCCACGGAGTCTCAAACCTGCAGGAATTTCGTTTCATGATTGGCGGGGGCTGCAC
TGAAGAGGATGCACTGTGATACTCTCCAGGAACCAGGGACTCTGACCCTGAGCCAGGCCTGCAGGTCCTG
CAGGCCTTCTTACTGGCTGTCGGTGCCAGTGCCAGAGAAGCCCCAAAACTTGGACAGCCTCATGGGCCTT
GTGTT
Notes:The probe template was PCR amplified from IMAGE:2749169 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2749169 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17049 same embryo
 EMAGE:17051 same embryo
 EMAGE:17047 same embryo
 EMAGE:17050 same embryo
 EMAGE:17046 same embryo
 EurExpress:euxassay_001439 same experiment
 MGI:4823010 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS