EMAGE mouse gene expression spatial database

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EMAGE:17459

Itgb7 integrin beta 7 ( MGI:96616)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003907. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003907_01	euxassay_003907_02	euxassay_003907_03	euxassay_003907_04

euxassay_003907_05	euxassay_003907_06	euxassay_003907_07	euxassay_003907_08	euxassay_003907_09

euxassay_003907_10	euxassay_003907_11	euxassay_003907_12	euxassay_003907_13	euxassay_003907_14

euxassay_003907_15	euxassay_003907_16	euxassay_003907_17	euxassay_003907_18	euxassay_003907_19

euxassay_003907_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17459_wholemount_strong.wlz

17459_wholemount_moderate.wlz

17459_wholemount_weak.wlz

17459_wholemount_possible.wlz

17459_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17459_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 08 09 11 12 weak expression: see section 07 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7306
Entity Detected:	Itgb7, integrin beta 7 ( MGI:96616)
Sequence:	sense strand is shown >T7306 GTACACAAGGGCTGCCACTGCCACCGGCGGAGGAAGGGCTGCTCCTCCTCAAGCACCTGCCATGGTGGAT TCATCAACTGTTCTCATTTTTCTGCTGGTTCTGGGCGGAGGTCAGAGTGAGTTAGATACCAAGATTACAT CCTCTGGGGAGGCCGCAGAATGGGAGGATCCTGACCTGTCTCTGCAGGGATCCTGCCAGCCAGTTCCTTC CTGCCAGAAGTGTATTCTCTCACATCCCAGCTGTGCATGGTGCAAACAACTGAACTTCACTGCCTCGGGA GAGGCAGAGGCGAGGCGCTGCGCGCGGCGAGAGGAGCTACTGGCTCGCGGGTGCCCCGCGCAGGAGCTAG AGGAGCCGAGAGGCCGCCAGGAGGTGCTGCAGGACAAGCCGCTCAGCCAGGGAGATCGCGGCGAGGGGGC CACCCAGCTGGCCCCGCAGCGGATCCGCGTCACGCTGCGACCAGGGGAGCCCCAGAAATTCCGGGTCCGC TTCCTCCGAGCTGCGGGGTACCCCGTGGACCTATATTACCTTATGGACCTGAGCTACTCAATGAAGGACG ACTTGGAACGTGTGCGCCAGCTAGGGCATGCCCTGCTGGTCAGGCTGCAGGAGGTCACACATTCTGTGCG CATAGGTTTTGGCTCCTTCGTGGACAAAACGGTGCTGCCCTTTGTAAGCACCGTGCCCTCCAAGCTTCAC CACCCATGCCCCAGCCGACTGGAGCGTTGTCAGCCACCCTTCAGCTTTCACCACGTGCTGTCCCTCACCG GGGACGCTCAAGCCTTCGAGAGAGAGGTGGGACGCCAGAATGTCTCTGGCAACCTGGACTCACCCGAAGG CGGCTTTGATGCCATTTTGCAGGCTGCCCTCTGCCAGGAACAGATTGGCTGGAGAAATGTCTCCCGTCTT CTAGTGTTCACTTCAGACGATACATTCCACACAGCTGGGGATGGGAAACTGGGTGGCATTTTCATGCCCA GCGACGGGCGGTGCCATCTGGACAGCAATGGTGTCTACACGAACAGCGCAGAGTTTGACTATCCCTCTGT GGGTCAAGTAGCCCAGGCCCTCACTGCAGCGAATATCCAACCTATCTTTGCTGTCACCGGTGCAACACTG CCTGTGTATCAGGAGCTGAGACAGTTGATTCCCAAGTCTGCTGTCGGGGAGCTGAGTGAGGACTCCAGCA ATGTGGTGCAGCTCATCATGGATGCTTATGATAGCCTGTCATCCACTGTGACTCTTGAGCACTCTCCACT CCCACCAGGAGTCAGCATCTCCTTTGAATCTCACTGTAAGGGTCCTGAGAAGACGGAGGGTGAGGCTGGG GACCGGGGACAGTGCAATGATGTCCGAGTCAACCAGACGGTGGATTTCTGGGTCACTCTTCAAGCTACTC ACTGCCTCCCAGAAGCCCACGTCCTACGACTCTGGGCTCTTGGCTTCTCAGAGGAGTTAACTGTGGAGCT GCACACAGTGTGCGACTGTAACTGTGGTGATGCCCAGCCTCACGCTCCCTACTGCAGTGATGGACAGGGG GACCTTCAGTGCGGGATATGCAGCTGTGCCCCTGGCCGTCTTGGTCAGCTGTGTGAATGCTCTGAGGCTG ACCTGTCCTCCCCAGATTTGGAATCTGGTTGCCGGGCCCCAAACGGGACAGGGCCCCTATGCAGCGGGAA GGGTCGATGCCAATGTGGACGCTGCAGCTGCAGTGGGCAGAGCTCTGGGCGTCTGTGCGAGTGTGATGAT GCCAGCTGTGAGCGACATGAGGGCATCCTCTGTGGAGGCTTTGGCCACTGCCAGTGTGGAGTATGTCACT GTCACGCCAACCACACCGGCAGAGCTTGCGAGTGCAGCAAGAGTGTAGACAGCTGTGTCAGTCCTGAAGG AGGGCTCTGCAGTGGGCACGGATACTGCAAATGCAACCGTTGCCAGTGCCTGGATGGCTACTACGGGGCT CTGTGTGACCAGTGCCTAGGCTGCAAGTCACCATGTGAGCAGTACAGGGATTGTGCAGAGTGCGGGGCAT TTGGTACCGGTCCCCTGGCAGCCAACTGTAGTGTGGTCTGTGCTGACGTGAATGTGACCTTGACCTTGGC CCCTAACTTGGATGATGGCTGGTGCAAAGAAAGGACAATAGACAACCAGCTGTTCTTCTTCCTGGTGGAG CATGCAGCCAGCGGGATCGTACTGAGAGTGAGACCCCAAGAGAAGGGAGTGGATCACACCCGTGCCATCA TACTGGGCTGCACAGGGGGCATCGTGGCAGTGGGACTAGGGCTGGTTCTGGCTTACCGGCTCTCTGTGGA AATCTACGACCGACGGGAGTACAGGCGCTTTGAGAAGGAGCAGCAGCAACTCAACTGGAAGCAGGACAAC AATCCTCTCTACAAAAGTGCGATCACAACCACTGTCAACCCCCGCTTCCAAGGGACAAACGGTCGGTCGC CATCCCTCTCTCTGACCAGGGAAGCAGACTGACTTAGGATTCTTGTCTTGGAGGACAGTGGAGATAGAAG GGCAGGGCAGCGTCTGTCAGGCAAAGATGCTGCCACCGCTGAGATTTTTCAGAGTGACCTTCAGAGGGCA GCAGCCATTCCCACCACACGAAGGGCTGGTCCTTCCATAATAAAGCTTTGCCCCAGAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4187781 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4187781 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17461 same embryo
	EMAGE:17458 same embryo
	EMAGE:17460 same embryo
	EMAGE:17457 same embryo
	EurExpress:euxassay_003907 same experiment
	MGI:4825657 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source