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EMAGE:17749

En2 engrailed 2 ( MGI:95390)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749
"Pseudo-wholemount" of euxassay_008914. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008914_01 euxassay_008914_02 euxassay_008914_03 euxassay_008914_04
EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749
euxassay_008914_05 euxassay_008914_06 euxassay_008914_07 euxassay_008914_08 euxassay_008914_09
EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749
euxassay_008914_10 euxassay_008914_11 euxassay_008914_12 euxassay_008914_13 euxassay_008914_14
EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749 EMAGE:17749
euxassay_008914_15 euxassay_008914_16 euxassay_008914_17 euxassay_008914_18 euxassay_008914_19
EMAGE:17749 EMAGE:17749 EMAGE:17749
euxassay_008914_20 euxassay_008914_21 euxassay_008914_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17749Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17749_wholemount_strong.wlz
17749_wholemount_moderate.wlz
17749_wholemount_weak.wlz
17749_wholemount_possible.wlz
17749_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17749_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 weak expression: see section 15
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 weak expression: see section 02 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35438
Entity Detected:En2, engrailed 2 ( MGI:95390)
Sequence:sense strand is shown

>T35438
AAAGGGGACTGTTTAGGGTTTCAGATCTTTCTTTCATTTTTCTTTTCTCCCTTTTTTTAATCCCTTAAAC
TCCTTATGGGACATTGGACACTTCTTCCCCCGAAAGAAAAAAAATTTAAAACAACTTGCTGAAGTCCAAA
GATTTTTATTGCTGCATTTCACAGGACTGTGAACCGAATAAATAGCTCCTATTTGGTCTGTGGGCTCTGC
CGCTTGCTTTGTGCTGGCTCGGACAGGTACTGTAGGAAGCGCCAGCCAGCGTGGTGATGGGAGAGCTCCT
GGTGGGGAGAGAGGGCGCAGTTCTTTGCTGAGGTCTGAGAAAGTCCTTGCAGGCCAGCAGCTCTCCGGGA
ACCTCTCCGCCTGCTTCCTACAGGGCCTCTCTTCTCAACCATCTCAGAGGCCCGTGCTGGCAGCCGCCGT
GTGTCTGCGTCTGTGTCGATGACCCCTGTCCGCCACAGCCCCGTAGCGACCTGCCAGCGCCAACTCTCCC
GCCACCGTGGCCAGCGTTGGGGCCTGGGGAGCAGTGGGAGTGTGTCCTGAAGGCTTGAAGGCTTTGCTGC
CGGGACAGCTAGCCCTGACCCAAAAGCCCTCACCGAGATAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89946. Forward Primer - name:089946_F_cDNA_En2, sequence:AAAGGGGACTGTTTAGGGTTTC; Reverse Primer - name:089946_N_SP6_cDNA_En2, sequence:CCTATCTCGGTGAGGGCTTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17752 same embryo
 EMAGE:17748 same embryo
 EMAGE:17751 same embryo
 EMAGE:17747 same embryo
 EMAGE:17750 same embryo
 EurExpress:euxassay_008914 same experiment
 MGI:4824550 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS