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EMAGE:18014

Dmxl2 Dmx-like 2 ( MGI:2444630)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014
"Pseudo-wholemount" of euxassay_011776. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011776_01 euxassay_011776_02 euxassay_011776_03 euxassay_011776_04
EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014
euxassay_011776_05 euxassay_011776_06 euxassay_011776_07 euxassay_011776_08 euxassay_011776_09
EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014
euxassay_011776_10 euxassay_011776_11 euxassay_011776_12 euxassay_011776_13 euxassay_011776_14
EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014 EMAGE:18014
euxassay_011776_15 euxassay_011776_16 euxassay_011776_17 euxassay_011776_18 euxassay_011776_19
EMAGE:18014 EMAGE:18014
euxassay_011776_20 euxassay_011776_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18014Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18014_wholemount_strong.wlz
18014_wholemount_moderate.wlz
18014_wholemount_weak.wlz
18014_wholemount_possible.wlz
18014_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18014_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19 20
facial vii ganglion
weak weak
regionalweak expression: see section 01 02 03 15 16
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 02 12
trigeminal v ganglion
weak weak
regionalweak expression: see section 01 02 03 04 05 14 15 16 17 18
vagus x ganglion
weak weak
regionalweak expression: see section 03 11
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 03
spinal cord
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 04 10
cervical ganglion
weak weak
regionalweak expression: see section 03 11
thoracic ganglion
weak weak
regionalweak expression: see section 07 08
dorsal root ganglion
weak weak
regionalweak expression: see section 01 02 03 04 05 09 10
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37689
Entity Detected:Dmxl2, Dmx-like 2 ( MGI:2444630)
Sequence:sense strand is shown

>T37689
CACATACCCAACTGACCTCTCAGTGGGAGCGGGGCCAGCTATTCTCCGAAATAAAGCAATGTTAGAGCCG
GAAAACACTCCATTCAAATCCCGGGATTCGTCTGCACTTCCTGTCAAGCGACTCTGGCATTTCCTTGTCA
AACAGGAAGTTCTTCAGGAGACATTTATTAGATACATTTTCACTAAGAAAAGAAAGCAGAGTGAGTCTGT
AGAAGAACATGTGGAGCAGGTCAAACACAACTCCGTAGCAGAAGATTGCCACATCAAGGTCGAAGCTGAT
CTGGGCTACCCAGGAGGGAAGGCGAAGGTCATTCATAAGGAATCTGACATGATCATGGCATTCTCTATTA
ATAAGGCAAACTGTAATGAAATTGTTTTGGCTTCAACACATGATGTTCAAGAACTCGATGTCACTTCTCT
ACTGGCTTGTCAATCATATATATGGATCGGAGAAGAATATGACAGAGAGTCGAAAAGTTCAGATGATATT
GATTACCGTGGTTCCACTACAACCCTGTACCAGCCTGGTGCAGCCTCCCATTCCTCAAGCCAGCCACACC
CACCTCCATCTCTGCCATGGCTAGGCAGTGGACAGACCAGCACTGGGGCTACTGTGCTCATGAAAAGGAA
TC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81933. Forward Primer - name:081933_F_cDNA_E130119P06Rik, sequence:CACATACCCAACTGACCTCTCA; Reverse Primer - name:081933_N_SP6_cDNA_E130119P06Rik, sequence:GATTCCTTTTCATGAGCACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18015 same embryo
 EMAGE:18012 same embryo
 EMAGE:18013 same embryo
 EurExpress:euxassay_011776 same experiment
 MGI:4824336 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS