EMAGE mouse gene expression spatial database

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EMAGE:18241

Adcy3 adenylate cyclase 3 ( MGI:99675)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012018. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012018_01	euxassay_012018_02	euxassay_012018_03	euxassay_012018_04

euxassay_012018_05	euxassay_012018_06	euxassay_012018_07	euxassay_012018_08	euxassay_012018_09

euxassay_012018_10	euxassay_012018_11	euxassay_012018_12	euxassay_012018_13	euxassay_012018_14

euxassay_012018_15	euxassay_012018_16	euxassay_012018_17	euxassay_012018_18	euxassay_012018_19

euxassay_012018_20	euxassay_012018_21	euxassay_012018_22	euxassay_012018_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18241_wholemount_strong.wlz

18241_wholemount_moderate.wlz

18241_wholemount_weak.wlz

18241_wholemount_possible.wlz

18241_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18241_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

single cell

weak expression: see section 03 04

trigeminal v ganglion

weak

single cell

weak expression: see section 02 03 04 16 17 18 19 20 21

ventral grey horn

moderate

single cell

moderate expression: see section 09 13 14

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 09 10 11 12 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30687
Entity Detected:	Adcy3, adenylate cyclase 3 ( MGI:99675)
Sequence:	sense strand is shown >T30687 GGTGGTCTTCTCCAGCGACAAGCTGGCGCCCCTCATGGTGGCAGGTTTCGGTCTGGTGTTGGACATCATC CTTTTCGTGCTCTGTAAAAAGGGGCTGCTCCCGGACCGAGTGAGCCGCAAAGTGGTACCCTACCTGCTGT GGCTGCTCATCTCGGCCCAGATCTTCTCCTACCTGGGCCTGAACTTTTCACGGGCCCACGCAGCCAGTGA CACTGTGGGTTGGCAGGCTTTCTTTGTCTTCTCCTTCTTCATAACGCTGCCCCTCAGCCTCAGCCCCATC GTGATCATCTCTGTGGTCTCCTGTGTTGTGCACACGCTTGTGTTGGGGGTCACTGTGGCCCAGCAGCAGC AGGATGAGCTGGAAGGGATGCAGCTGCTGAGGGAGATCCTGGCCAACGTCTTCCTCTACCTGTGTGCTAT CATCGTGGGCATCATGTCCTACTACATGGCAGACCGCAAGCACCGCAAGGCCTTCCTGGAGGCCCGCCAG TCACTGGAGGTGAAGATGAATCTGGAGGAGCAGAGCCAGCAGCAGGAAAACCTTATGCTTTCCATCCTGC CCAAGCACGTGGCTGACGAGATGCTGAAGGACATGAAGAAGGACGAGAGCCAGAAGGACCAGCAGCAGTT CAATACCATGTACATGTACCGGCACGAGAATGTCAGCATCCTGTTTGCAGATATTGTGGGCTTTACCCAG CTGTCCTCTGCTTGCAGTGCCCAGGAGCTCGTGAAGTTACTCAACGAGCTCTTCGCCCGCTTTGACAAGC TGGCAGCCAAATACCACCAGCTGAGGATCAAGATCCTAGGCGACTGTTACTACTGCATCTGCGGCTTGCC TGACTACCGGGAGGACCATGCCGTGTGCTCCATCCTTATGGGGCTTGCCAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6830961), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59532. Forward Primer - name:059532_F_IRAV118_b11_Adcy3, sequence:GGTGGTCTTCTCCAGCGA; Reverse Primer - name:059532_R_SP6_IRAV118_b11_Adcy3, sequence:CATGGCAAGCCCCATAAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18240 same embryo
	EurExpress:euxassay_012018 same experiment
	MGI:4822999 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source