EMAGE mouse gene expression spatial database

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EMAGE:19379

Lap3 leucine aminopeptidase 3 ( MGI:1914238)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002166. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002166_01	euxassay_002166_02	euxassay_002166_03	euxassay_002166_04

euxassay_002166_05	euxassay_002166_06	euxassay_002166_07	euxassay_002166_08	euxassay_002166_09

euxassay_002166_10	euxassay_002166_11	euxassay_002166_12	euxassay_002166_13	euxassay_002166_14

euxassay_002166_15	euxassay_002166_16	euxassay_002166_17	euxassay_002166_18	euxassay_002166_19

euxassay_002166_20	euxassay_002166_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19379_wholemount_strong.wlz

19379_wholemount_moderate.wlz

19379_wholemount_weak.wlz

19379_wholemount_possible.wlz

19379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 17 moderate expression: see section 11 12 13 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 moderate expression: see section 10 11 12 13 14 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6025
Entity Detected:	Lap3, leucine aminopeptidase 3 ( MGI:1914238)
Sequence:	sense strand is shown >T6025 CCGGAGGTCCCGCCTCCAGGCGCGCGCCCTGCCACGCGCCACGTGCTCCTTCTCTGGAGGGCGGCGCCGA GGGCCGAGCCGAGAAGATGTACTTGCTGCCCCTTCCGGCTGCCGCGCGAGTCGCGCTCCGCCGCCTGGGA GTGAGAGGTCTCTGGGATCGGGGTCTGTCCACCGCAGACATGACGAAGGGCCTTGTTTTAGGAATTTATG CCAAAGACAAAGATGATGACCTGCCACAATTTACAAGTGCGGGAGAGAGTTTTAATAAGTTGGTGTCTGG AAAGCTGAGGGAAATGTTGAACATATCTGGACCTCCTCTCAAAGCAGGCAAAACCAGAACCTTCTATGGT CTGCATCAGGACTTCCCCAGCGTGGTGGTGGTCGGCCTTGGCAAGCGATCGGCTGGAGTTGATGACCAGG AAAACTGGCACGAAGGCAAAGAAAACATCAGAGCTGCTGTTGCAGCGGGATGCAGGCAGGTCCAAGACCT GGAGCTGCCGTCGGTGGAGGTGGATCCCTGTGGAGATGCGCAAGCTGCTGCAGAAGGAGCTGTGCTTGGT CTCTATGAGTACGATGACCTGAAGCAGAAGAAGAAGGTGGCTGTGTCGGCGAAGCTCCACGGAAGCGGTG ATCTCGAGGCCTGGGAGAAAGGGGTCCTCTTTGCTTCTGGACAGAACTTGGCCCGTCATCTGATGGAGTC TCCAGCCAATGAGATGACACCAACCAGATTCGCTGAAATCATTGAAAAAAACCTCAAAAGTGCCAGTAGT AAAACCAAGGTCCATATCAGACCCAAGTCCTGGATAGAGGAACAAGAAATGGGTTCGTTCCTAAGTGTAG CCAAGGGGTCTGAAGAACCACCAGTCTTCTTGGAAATCCACTACATGGGCAGCCCCAATGCAACCGAGGC ACCCCTGGTGTTTGTGGGGAAGGGCATTACCTTTGACAGTGGAGGTATCTCCATCAAGGCTTCTGCAAAC ATGGACCTCATGAGGGCTGATATGGGAGGAGCAGCAACAATATGCTCGGCCATTGTGTCTGCGGCAAAGC TCAACTTGCCCATTAACATCATTGGTTTGGCCCCTCTTTGTGAAAATATGCCTAGCGGCAAGGCCAACAA GCCAGGGGATGTCGTCAGAGCCAGGAATGGGAAGACCATCCAGGTTGATAACACTGACGCCGAGGGGAGG CTCATCCTGGCCGATGCACTCTGTTATGCACACACCTTTAATCCCAAGGTCATCATCAATGCTGCCACCT TAACAGGTGCCATGGATGTAGCCCTGGGGTCAGGTGCCACTGGAGTCTTTACCAATTCGTCCTGGCTGTG GAACAAACTATTTGAGGCCAGCGTAGAAACTGGGGACCGAGTCTGGAGGATGCCCCTCTTTGAGCATTAT ACAAGACAAGTCATAGATTGCCAGCTTGCTGATGTCAACAATCTTGGAAAATACAGATCTGCAGGTGCGT GTACAGCTGCCGCATTCCTGCGAGAGTTTGTGACACATACGAAGTGGGCACACTTAGACATAGCAGGAGT GATGACCAACAAAGATGAGATTCCCTACCTGCGGAAGGGGATGAGTGGGCGGCCCACGAGGACTCTGATT GAGTTCTTACTTCGATTCAGTAAAGACAGTTCTTAGTTCACTCACAGATTACTCCATCTGTCTTGAATTG GATATTTGACCTTCAAAATATTTTTGAATGAATGAAAATCTTTTAAAGACTAAAATGAATGTTGTTCTTT TTTTTAATCTTAATTTTTGGAATACTTTAATATGCTTGAATCATGGAAATCTAAATGATATATGACTTAA TATAGGCCCTTTAAACTATTAAAACATCTTGTAATAATATGCATTTTCAAGCCATATTTACTATTTTTAC AAGATAGGGGAAATCTTAAGTCCATAAAAATGGACAAAGGCCAGGCATGGGGTGCAGAACTTTGATCCCA GCATTTGGGAGGCAGAGGCAGCTCGATTTCTGAGTTTGAGACCATCGTGGTCTACAGAGTGAATTCCAGG ATAGCCATGGCTATACAGAGAAACCCTGTCTTGGAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488469 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488469 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19381 same embryo
	EMAGE:19378 same embryo
	EMAGE:19382 same embryo
	EMAGE:19380 same embryo
	EMAGE:19377 same embryo
	EurExpress:euxassay_002166 same experiment
	MGI:4825868 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source