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EMAGE:20156

Sppl3 signal peptide peptidase 3 ( MGI:1891433)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156
"Pseudo-wholemount" of euxassay_012501. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012501_01 euxassay_012501_02 euxassay_012501_03 euxassay_012501_04
EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156
euxassay_012501_05 euxassay_012501_06 euxassay_012501_07 euxassay_012501_08 euxassay_012501_09
EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156
euxassay_012501_10 euxassay_012501_11 euxassay_012501_12 euxassay_012501_13 euxassay_012501_14
EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156 EMAGE:20156
euxassay_012501_15 euxassay_012501_16 euxassay_012501_17 euxassay_012501_18 euxassay_012501_19
EMAGE:20156 EMAGE:20156
euxassay_012501_20 euxassay_012501_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20156Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20156_wholemount_strong.wlz
20156_wholemount_moderate.wlz
20156_wholemount_weak.wlz
20156_wholemount_possible.wlz
20156_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20156_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31349
Entity Detected:Sppl3, signal peptide peptidase 3 ( MGI:1891433)
Sequence:sense strand is shown

>T31349
CTCACTGGCCACTGGCTTCTCATGGATGCTCTGGCCATGGGTCTCTGTGTTGCCATGATCGCCTTCGTCC
GCCTGCCAAGCCTCAAGGTTTCCTGCCTGCTTCTCTCAGGGCTTCTCATCTACGATGTCTTCTGGGTGTT
CTTCTCAGCCTACATCTTCAACAGTAATGTCATGGTGAAAGTGGCCACACAGCCAGCTGACAATCCCCTC
GACGTTCTGTCCAGGAAGCTCCACCTGGGACCCAATGTGGGGCGTGATGTTCCTCGCCTGTCTTTGCCTG
GAAAATTGGTCTTCCCAAGCTCCACTGGCAGTCACTTCTCTATGCTGGGCATCGGGGACATTGTGATGCC
CGGCCTCCTGTTATGCTTTGTTCTTCGCTATGACAACTACAAGAAACAAGCCAGTGGTGACTCGTGTGGG
GCCCCTGGCCCAGCCAATATCTCTGGGCGCATGCAGAAGGTCTCCTACTTCCACTGCACCCTCATCGGGT
ACTTTGTAGGTCTGCTCACTGCGACTGTGGCGTCTCGCATCCACCGAGCTGCCCAGCCAGCTCTCCTCTA
CTTGGTGCCATTTACCCTATTGCCACTCCTCACCATGGCCTACCTAAAGGGTGACTTACGGAGGATGTGG
TCTGAGCCCTTCCACTCCAAGTCCAGCAGCTCCCGGTTCCTGGAAGTATGATGACGCGGGAGAGGTGACC
AGAGCGCTGTCCTCACCTTCCTCTCTCACCGCGCGGTTTTGTTCCCTCTACAAGCTGGCCTGACTCAGGC
ATGCCTGCTCACGGAACTGCAGTGTGACTGGAGTGTGTGTCTGAGGGGAGCGTTTGCAGAGGGCACTGGA
GCCCTGCACAGCCCTTCTCTTCTCCGCTCTGGGAGAGTGGACCCTTCCAGAAGGGGCAGGCCGGCCCTTC
GCCCGGTGCTCCTCCTTCCTCGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3154534), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 23855. Forward Primer - name:023855_F_IRAV51-54_O02_Sppl3, sequence:CTCACTGGCCACTGGCTT; Reverse Primer - name:023855_R_SP6_IRAV51-54_O02_Sppl3, sequence:CACGAGGAAGGAGGAGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20158 same embryo
 EMAGE:20159 same embryo
 EMAGE:20157 same embryo
 EMAGE:20155 same embryo
 EurExpress:euxassay_012501 same experiment
 MGI:4828425 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS