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EMAGE:20247

Itih2 inter-alpha trypsin inhibitor, heavy chain 2 ( MGI:96619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247
"Pseudo-wholemount" of euxassay_012380. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012380_01 euxassay_012380_02 euxassay_012380_03 euxassay_012380_04
EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247
euxassay_012380_05 euxassay_012380_06 euxassay_012380_07 euxassay_012380_08 euxassay_012380_09
EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247
euxassay_012380_10 euxassay_012380_11 euxassay_012380_12 euxassay_012380_13 euxassay_012380_14
EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247 EMAGE:20247
euxassay_012380_15 euxassay_012380_16 euxassay_012380_17 euxassay_012380_18 euxassay_012380_19
EMAGE:20247 EMAGE:20247
euxassay_012380_20 euxassay_012380_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20247Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20247_wholemount_strong.wlz
20247_wholemount_moderate.wlz
20247_wholemount_weak.wlz
20247_wholemount_possible.wlz
20247_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20247_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindbrain meninges
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
spinal cord meninges
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31263
Entity Detected:Itih2, inter-alpha trypsin inhibitor, heavy chain 2 ( MGI:96619)
Sequence:sense strand is shown

>T31263
GCCTAGTGGAGGCACGAATATCAACGAGGCACTGCTTCGAGCAATTTTCATTTTGAATGAAGCCAGTAAC
ATGGGATTGCTAAACCCCGACTCAGTCTCTCTGATCATTTTGGTTTCTGATGGAGATCCAACCGTAGGGG
AACTAAAATTGTCCAAAATTCAGAAAAATGTGAAGCAGAGCATCCAAGATAACATCTCCCTGTTTAGTTT
GGGAATCGGATTTGATGTCGACTATGATTTTTTGAAGAGGCTGTCCAATGAAAATCGTGGTATTGCTCAA
CGGATCTACGGGAACCAGGACACATCCTCTCAGCTCAAGAAATTTTACAACCAGGTCTCTACTCCACTGC
TCAGAAATGTTCAGTTTAACTACCCGCAGGCATCAGTAACAGATGTCACCCAAAACAATTTCCACAACTA
CTTCGGAGGTTCTGAGATCGTGGTGGCAGGGAAATTTGATCCCAGCAAACTGACAGAAGTCCAGAGCATC
ATCACGGCAACTTCGGCTAACACAGAATTGGTCTTGGAAACCCTGAGCCAGATGGATGACTTGGAGGAGT
TTCTGTCAAAGGACAAGCATGCAGACCCTGACTTCACCAAGAAGCTGTGGGCCTATCTCACCATCAACCA
ACTACTAGCAGAGAGAAGTCTGGCTCCTACTGCTGCCATCAAAAGGAAAATCACCAAAACAATCCTGCAG
ATGTCTCTAGACCATCACATCGTGACTCCGCTTACTGCCATGGTGATAGAGAATGATGCTGGAGATGAAC
GCATGCTGGCTGACTCCCCGCCACAGGACCACTCCTGCTGCTCAGGCGCACTGTATTATGGCACCAAGGT
TGCCTCGGGTCCCATCCCATCATGGGCCAACCCATCTCCGACAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4211576), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54279. Forward Primer - name:054279_F_IRAV49_e10_Itih2, sequence:GCCTAGTGGAGGCACGAA; Reverse Primer - name:054279_R_SP6_IRAV49_e10_Itih2, sequence:GGTGTCGGAGATGGGTTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20248 same embryo
 EMAGE:20251 same embryo
 EMAGE:20249 same embryo
 EMAGE:20246 same embryo
 EMAGE:20250 same embryo
 EurExpress:euxassay_012380 same experiment
 MGI:4825661 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS