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EMAGE:20469

Pik3cd phosphatidylinositol 3-kinase catalytic delta polypeptide ( MGI:1098211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469
"Pseudo-wholemount" of euxassay_012716. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012716_01 euxassay_012716_02 euxassay_012716_03 euxassay_012716_04
EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469
euxassay_012716_05 euxassay_012716_06 euxassay_012716_07 euxassay_012716_08 euxassay_012716_09
EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469
euxassay_012716_10 euxassay_012716_11 euxassay_012716_12 euxassay_012716_13 euxassay_012716_14
EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469 EMAGE:20469
euxassay_012716_15 euxassay_012716_16 euxassay_012716_17 euxassay_012716_18 euxassay_012716_19
EMAGE:20469
euxassay_012716_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20469Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20469_wholemount_strong.wlz
20469_wholemount_moderate.wlz
20469_wholemount_weak.wlz
20469_wholemount_possible.wlz
20469_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20469_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 12 13
brain
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 17 18 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 16
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 15 16 17 18 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 16 17
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 07 14
spinal cord
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 08 14
cervical ganglion
moderate moderate
regionalmoderate expression: see section 07 weak expression: see section 08 15
thoracic ganglion
weak weak
regionalweak expression: see section 10
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 19 20
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 weak expression: see section 06 07 09 11 12 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38211
Entity Detected:Pik3cd, phosphatidylinositol 3-kinase catalytic delta polypeptide ( MGI:1098211)
Sequence:sense strand is shown

>T38211
GAGTTCGATATCAGCGTCTGTGACCTCCCGCGCATGGCTCGACTCTGTTTTGCTCTCTATGCCGTCGTGG
AGAAGGCTAAGAAGGCACGCTCCACAAAGAAGAAGTCTAAGAAGGCGGACTGCCCCATCGCTTGGGCCAA
CCTCATGCTATTCGACTACAAAGATCAGCTCAAGACGGGGGAGCGCTGCCTCTACATGTGGCCCTCTGTC
CCAGATGAGAAGGGAGAGCTGCTGAATCCTGCGGGTACAGTGCGCGGGAACCCCAACACGGAGAGTGCCG
CTGCCCTGGTCATCTACCTGCCTGAGGTGGCCCCCCACCCTGTGTACTTCCCCGCTCTGGAGAAGATCCT
GGAGCTGGGGCGTCACGGGGAGCGTGGGCGCATCACGGAGGAGGAGCTGCAGCTGCGGGAGATCCTGGAA
CGGCGGGGATCCGGGGAACTGTACGAACATGAGAAGGACCTGGTGTGGAAGATGCGCCACGAAGTCCAGG
AGCATTTCCCAGAGGCGCTGGCCCGCCTGCTGCTGGTCACCAAGTGGAATAAACACGAGGATGTGGCCCA
GATGCTCTATTTGCTGTGCTCCTGGCCCGAGCTGCCTGTGCTGAGCGCCCTGGAACTTCTGGACTCTAGC
TTTCCCGACTGCTACGTGGGCTCCTTCGCCATCAAGTCCCTTCGGAAGCTGACGGACGATGAGCTCTTCC
AGTACCTTCTGCAGCTGGTGCAAGTGCTCAAATATGAGTCCTACCTGGACTGCGAGCTGACCAAATTCTT
GCTGGGCCGAGCCCTGGCTAACCGCAAGATCGGACACTTCCTGTTCTGGCACCTCCACTCTGAGATGCAC
GTACCATCAGTGGCTCTGCGGTTTGGTCTCATCATGGAAGCCTACTGCAGAGGCAGCACCCACCACATGA
AGGTGCTGATGAAGCAGGGGGAAGCACTGAGCAAGCTTAAGGCACTGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 147045. Forward Primer - name:147045_F_cDNA_Pik3cd, sequence:GAGTTCGATATCAGCGTCTGTG; Reverse Primer - name:147045_N_SP6_cDNA_Pik3cd, sequence:TTCAGTGCCTTAAGCTTGCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20465 same embryo
 EMAGE:20464 same embryo
 EMAGE:20468 same embryo
 EMAGE:20467 same embryo
 EMAGE:20466 same embryo
 EurExpress:euxassay_012716 same experiment
 MGI:4827207 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS