| Type: | in situ hybridisation probe |
| Identifier: | MGI:3768770 |
| Entity Detected: | Col5a1, collagen, type V, alpha 1 ( MGI:88457) |
| Sequence: | sense strand is shown
>MGI:3768770
TTTCCTGGAGATCCTGGTCCCCCTGGAGAGCCAGGCCCCGCAGGTCAAGACGGCCCACCTGGTGACAAAG
GGGACGATGGTGAACCTGGGCAGACGGGGTCCCCGGGCCCTACTGGTGAACCTGGTCCATCTGGGCCTCC
AGGAAAGAGGGGTCCCCCAGGCCCTGCAGGCCCTGAAGGCAGGCAGGGGGAGAAAGGAGCCAAGGGAGAA
GCTGGCTTAGAAGGCCCTCCTGGGAAGACTGGCCCCATTGGCCCCCAAGGGGCCCCTGGGAAGCCTGGCC
CCGATGGTCTCCGTGGAATCCCTGGTCCTGTGGGTGAGCAAGGCCTCCCAGGATCCCCAGGCCCCGATGG
TCCACCCGGCCCCATGGGTCCTCCAGGACTCCCTGGCCTCAAAGGAGACTCCGGTCCCAAAGGTGAAAAG
GGCCATCCAGGCCTGATTGGACTCATCGGCCCTCCGGGAGAGCAAGGTGAAAAGGGTGACCGTGGACTCC
CAGGCCCCCAGGGTTCATCTGGTCCTAAAGGAGATCAGGGCATCACAGGTCCTTCTGGCCCACTTGGGCC
TCCTGGTCCTCCTGGCTTGCCGGGCCCTCCAGGTCCCAAAGGTGCTAAGGGCTCTTCGGGTCCCACCGGC
CCGAAGGGTGAGGCAGGCCACCCAGGACTCCCCGGCCCACCTGGCCCTCCGGGTGAGGTCATCCAGCCCC
TGCCAATCCAGGCCTCCAGGACTCGGCGGAACATTGATGCCAGCCAGCTCCTGGACGATGGGGCTGGGGA
GAGCTACGTGGATTATGCAGATGGCATGGAAGAGATCTTTGGTTCCCTCAACTCCCTGAAGCTGGAGATT
GAACAGATGAAGCGACCACTGGGCACCCAGCAGAACCCAGCCCGTACCTGCAAGGATCTACAGCTCTGTC
ATCCTGACTTCCCAGATGGCGAATACTGGGTCGATCCCAACCAAGGGTGCTCCAGGGACTCCTTCAAAGT
CTACTGCAATTTCACAGCTGGAGGGTCCACGTGCGTCTTCCCTGACAAGAAGTCTGAGGGAGCCAGAATC
ACTTCTTGGCCCAAAGAAAACCCAGGTTCCTGGTTCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATG
TGGATGCTGAAGGC
|
| | nt 4418 - nt 5551 of NM_015734.2 |
| Notes: | The Col5a1 probe used in this study by Wenstrup et al., 2004 [PMID:15383546] is described as "extending from exon 51 to part of exon 65 of col5a1. This was accomplished either by using a PCR strategy in which the bacterial T7 promoter sequence was added to the 5' end of the antisense sequence for the transcription of an antisense RNA probe or by subcloning into a vector with the T7 promoter for the sense probe. The primer pair for the probe were as follows: col5a1 antisense, 5'-GGTTTCCTGGAGATCCTGGC-3' and 5'-GATCCTAATACGACTCACTATAGGGAGGGCCTTCAGCATCCAC-3'." |
| Chemistry: | RNA |
| Strand: | antisense |
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