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EMAGE:672

Sox10 SRY-box containing gene 10 ( MGI:98358)
TS17 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:672

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The embryo has approx. 30 somites.
Expression Pattern Description
Spatial Annotation:
EMAGE:672Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
672_wholemount_strong_3D_1.wlz
672_wholemount_moderate_3D_1.wlz
672_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:672_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
otocyst
strong strong
regionalExpression detected in otic vesicle.
dorsal root ganglion
strong strong
regionalExpression in the trunk is observed at the level of the anterior halves of the somites (ventrally migrating neural crest) before and during the formation of dorsal root ganglia condensations and the sympathetic ganglia.
acoustic viii ganglion
strong strong
Expression is described as being in the acoustic ganglion.
trigeminal v ganglion
strong strong
Expression is described as being in the trigeminal ganglion
glossopharyngeal ix ganglion
strong strong
Expression is described as being in the superior ganglion ie. proximal IX or glossopharyngeal ganglion.
vagus x ganglion
strong strong
Expression is described as being in the jugular ganglion ie. proximal/superior X or vagus ganglion.
facial vii ganglion
strong strong
Expression is described as being in the geniculate ie. distal VII (facial) ganglion.
nasal epithelium
moderate moderate
regionalExpression detected in olfactory placode.
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sox10 probeB
Entity Detected:Sox10, SRY-box containing gene 10 ( MGI:98358)
Sequence:sense strand is shown

>Sox10 probeB
CAGGACGGCGAGGCAGACGATGACAAGTTCCCGGTGTGCATCCGCGAGGCGGTCAGCCAGGTGCTCAGCG
GCTACGACTGGACGCTGGTGCCCATGCCCGTGCGGGTCAACGGTGCCAGCAAGAGCAAGCCGCACGTCAA
ACGGCCCATGAACGCCTTTATGGTGTGGGCACAGGCGGCACGCAGAAAGTTAGCCGACCAGTACCCGCAC
CTCCACAATGCTGAGCTCAGCAAGACGCTGGGCAAGCTCTGGAGGTTGCTGAACGAGAGTGACAAGCGCC
CCTTCATTGAGGAGGCCGAGCGGCTCCGGATGCAGCACAAAAAAGACCATCCGGACTACAAGTACCAACC
TCGGCGGCGGAAGAATGGGAAGGCAGCCCAGGGGGAGGCAGAGTGCCCAGGCGGGGAGACCGACCAAGGA
GGGGCTGCTGCTATCCAGGCTCACTACAAGAGTGCCCACCTGGACCACCGGCACCCAGAAGAAGGCTCCC
CCATGTCAGATGGGAACCCAGAGCACCCCTCAGGCCAGAGCCATGGCCCTCCTACCCCTCCAACCACCCC
AAAGACAGAGCTGCAGTCCGGCAAGGCAGATCCCAAAAGGGATGGGCGCTCCTTGGGGGAGGGCGGGAAG
CCCCACATCGACTTCGGCAATGTGGACATCGGGGAGATCAGCCATGAGGTAATGTCCAACATGGAGACCT
TTGATGTGACTGAGCTGGACCAATACCTGCCGCCCAATGGGCACCCAGGCCACGTGGGCAGCTACTCGGC
AGCTGGCTACGGGCTGAGCAGTGCCCTGGCTGTGGCCAGTGGACACTCTGCCTGGATCTCCAAGCCACCA
GGTGTGGCTCTGCCCACGGTCTCGCCCCCTGCTGTGGATGCCAAAGCCCAGGTGAAGACAGAGACCACAG
GGCCCCAGGGACCCCCACACTACACCGACCAGCCGTCCACTTCCCAGATCGCCTACACCTCCCTCAGTCT
GCCCCACTACGGCTCCGCCTTCCCCTCCATCTCACGCCCCCAGTTTGACTACTCTGACCATCAGCCCTCA
GGACCCTATTATGGCCACGCAGGCCAGGCCTCTGGCCTCTACTCAGCTTTTTCCTACATGGGCCCCTCTC
AGCGGCCCCTCTACACGGCCATCTCTGACCCCAGCCCCTCAGGGCCCCAGTCCCACAGTCCCACACACTG
GGAACAGCCAGTATATACGACTCTATCCCGACCTTAGAGGGGCCCTGTCACCATTGGTGCCCACGTGGCC
CAGGGCCCCTTCCCCCAGCCTGTGTGCCCTGCTCCTCATCAGCCTCATGCCCAGCAGTGGCAGTGGAGGA
GGCTGCAGAGGCTGACAGCAGCAGGCAGGCTTCTGCCAGCTCCTGCCCTGATGACCTCCACCCACCCTGG
CTCGGGAGGCCAAGCCCTGACTGAGCTGGCAAAGGAGGAGTTTGGTGGCCATCCCAGACTGAGGGTGAGG
GGCCACCCATCCCCAGAAATAACCCTCTATCCCAGGACCTGAGAAGTCGTTCTCAAGTCTTTCAGGGAGG
CAGAAAAGGGTATGGTGGGAAACCTCACTGCTCCTGTTCTCTCCTGGGAGAGCAAGGGGCTCAAGGGCCT
GTGTGCCACTGTGTCACTGTGCCTAAAAGCTAGGCTGCCTAGGGACCTGCTTCAAAGCCTGGAGCTGGCT
CTGTCCTGTCACTAAGGACTCACTGAGGACAGCTTTGAACAGCTTTGTGAAGAGCAGGGTGGGACACTCA
ATGGACACTCTGATCCTTTCACCCCCCTCCTCACCTGAATGGGAACTGGCCGAGGTCCCCAGATCCAGTT
CTGCATTAGTAACCTCATCCCTCACCTAACTGAGGAGAGTCCTCATGTTCTTCCCCACGACCTCCACTCC
TCAAGGCCCACCCCAGGTGAGGAGGCAGGGGCTCCAGGAAAGAATCAGAGGTGTCCAGCCTTCCTCTCGC
CTTCTGTCAGCCTCAGTTAGCACTGCACGGGCCCTAGCCCCAGAGCCCAGTACCTGGGTTGCTGGCAGCA
GGCTGGACACTAAACCCCTTCCATGTACAGTCTACCTTTGCCTCGCACCCTTTGCTCCAGCGATATCTTA
ATAAAGTACGTAGCTCTGTCTCCCCATCTCCTGTCTGCAGCCCAGTCCACACGTAACACATCGCTGCCCC
TTTATCTCCACAGTCACCATGTTCCCATCCCTCTAGCTACTCCAGCCCCAATTAACTTGCATTAAGCACT
CCAAGTAATAAAATCAAAGCTTCTGG
nt 757 - nt 3022 of NM_019193.1
Notes:Probe constructed by Kristen Kuhlbrodt in the lab of Michael Wegner in 08/1996, and was obtained by screening a random CG4 (undifferentiated) - cDNA library from rat with a SX10-2 probe. Insert contains nt 757-3022 of rat Sox10 (ORF: nt 583 to 1983). The probe was hydrolysed to ~500 bp.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57B/6 x 129/B6
Age:9.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Edward Coles & Marianne Bronner-Fraser
Principal investigator:Marianne Bronner-Fraser, California Institute of Technology, Division of Biology 139-74, Pasadena, USA CA 91125
Submitted by:Marianne Bronner-Fraser, California Institute of Technology, Division of Biology 139-74, Pasadena, USA CA 91125
Experiment type:non-screen
Acknowledgments:The probe used in this study was a kind gift from Kristen Kuhlbrodt and Michael Wegner.  Kristen Kuhlbrodt, Michael Wegner  Zentrum f�r Molekulare Neurobiologie, Universit�t Hamburg,, Hamburg, 20246, Germany
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE