EMAGE mouse gene expression spatial database

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EMAGE:7110

Gadd45b growth arrest and DNA-damage-inducible 45 beta ( MGI:107776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002596. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002596_01	euxassay_002596_02	euxassay_002596_03	euxassay_002596_04

euxassay_002596_05	euxassay_002596_06	euxassay_002596_07	euxassay_002596_08	euxassay_002596_09

euxassay_002596_10	euxassay_002596_11	euxassay_002596_12	euxassay_002596_13	euxassay_002596_14

euxassay_002596_15	euxassay_002596_16	euxassay_002596_17	euxassay_002596_18	euxassay_002596_19

euxassay_002596_20	euxassay_002596_21	euxassay_002596_22	euxassay_002596_23	euxassay_002596_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7110_wholemount_strong.wlz

7110_wholemount_moderate.wlz

7110_wholemount_weak.wlz

7110_wholemount_possible.wlz

7110_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7110_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 06 07 08 10 18 19 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5216
Entity Detected:	Gadd45b, growth arrest and DNA-damage-inducible 45 beta ( MGI:107776)
Sequence:	sense strand is shown >T5216 CCACGCGTCCGGGGTCTGCGTTCATCTCTGTCTTCTTGGATTAATTTCGAGGGGGATTTTGCAATCTTCT TTTTACCCCTACTTTTTTCTTGGGAAGGGAAGTCCCACCGCCTCCGGAAGGCCTCCGACACTTCTGGTCG CACGGGAAGGTTTTTTTGCCTCTTGGGTTCGTATCTGGACTTGTACTTTGCTCTTGGGGATCTTCCGTGG GGGTCCGCTGTGGAGTGTGACTGCATCATGACCCTGGAAGAGCTGGTGGCGAGCGACAACGCGGTTCAGA AGATGCAGGCGGTGACTGCCGCGGTGGAGCAGCTGCTGGTGGCCGCGCAGCGTCAGGATCGCCTCACCGT GGGGGTGTACGAGGCGGCCAAACTGATGAATGTGGACCCCGACAGCGTGGTCTTGTGCCTCCTGGCCATA GACGAAGAAGAGGAGGATGATATCGCTCTGCAGATTCACTTCACCCTGATCCAGTCGTTCTGCTGCGACA ATGACATTGACATCGTCCGGGTATCAGGCATGCAGAGGCTGGCGCAGCTCCTGGGGGAGCCGGCGGAGAC ATTGGGCACAACCGAAGCCCGAGACCTGCACTGCCTCCTGGTCACGAACTGTCATACAGATTCCTGGAAA AGCCAAGGCTTGGTGGAGGTGGCCAGTTACTGTGAAGAGAGCAGAGGCAATAACCAATGGGTCCCCTATA TCTCTCTAGAGGAACGCTGAGACCCACTCCAAACATCTAAAGCAACTGTCGAGTTGCTGTCCCCTAAAAA AAGTAAATAAAATACATATTTGACAGCCCCCTCATCCCCCAGAACAATCCCTCAAAGGCTACCCTACCCG TGATACCTTCTGGGAGGGGCGGAGTCACCGAGACTGAGATGAGGAGAGGGGCACGTGCGCCCGCCCGCCC TCTGGGCTGTGGAGCCAGGAGCAGCACCACAGGTGGTCGCCGAGGTCGGAAGGAGGGCACCTCAGGCAAG AGGAGACTGAGACTTTAGAGCCAAGGCCTGGCAGTCCTGCAGCCAGCCTCTGCTCGCAGCCGCAGACGGT CTGGACACCGCCGCAGGGGTGGGGTGAGGCGTCCCCCACCCTGCGGGACAGTGAACTGTGCATAAGTCAG CGGAGGGCGACGACCCTCGCCGCGGGACCCGGGACTCGAGCCCGGGACTTCGCAGCTACAGCACATCTAT TTTTAATATTGTGCTGAGCAAGACAGATCGCTTGCATATTTTTAAAAATTTCTACTACAGAGACATTCCA ATAAACTCGTTAAGCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5355150 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5355150 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7114 same embryo
	EMAGE:7109 same embryo
	EMAGE:7111 same embryo
	EMAGE:7113 same embryo
	EMAGE:7112 same embryo
	EurExpress:euxassay_002596 same experiment
	MGI:4824973 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source