EMAGE mouse gene expression spatial database

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EMAGE:7296

Sod2 superoxide dismutase 2, mitochondrial ( MGI:98352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004393. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004393_01	euxassay_004393_02	euxassay_004393_03	euxassay_004393_04

euxassay_004393_05	euxassay_004393_06	euxassay_004393_07	euxassay_004393_08	euxassay_004393_09

euxassay_004393_10	euxassay_004393_11	euxassay_004393_12	euxassay_004393_13	euxassay_004393_14

euxassay_004393_15	euxassay_004393_16	euxassay_004393_17	euxassay_004393_18	euxassay_004393_19

euxassay_004393_20	euxassay_004393_21	euxassay_004393_22	euxassay_004393_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7296_wholemount_strong.wlz

7296_wholemount_moderate.wlz

7296_wholemount_weak.wlz

7296_wholemount_possible.wlz

7296_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7296_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 02 03 04 19 20 21 22

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 08 09 10 11 14 15 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 17

dorsal root ganglion

moderate

regional

moderate expression: see section 06 08 09 10 11 12 13 15 16 17 weak expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6391
Entity Detected:	Sod2, superoxide dismutase 2, mitochondrial ( MGI:98352)
Sequence:	sense strand is shown >T6391 CGGCCGTGTTCTGAGGAGAGCAGCGGTCGTGTAAACCTCAATAATGTTGTGTCGGGCGGCGTGCAGCACG GGCAGGAGGCTGGGCCCTGTGGCCGGTGCCGCGGGCTCCCGGCACAAGCACAGCCTCCCAGACCTGCCTT ACGACTATGGCGCGCTGGAGCCACACATTAACGCGCAGATCATGCAGCTGCACCACAGCAAGCACCACGC GGCCTACGTGAACAATCTCAACGCCACCGAGGAGAAGTACCACGAGGCTCTGGCCAAGGGAGATGTTACA ACTCAGGTCGCTCTTCAGCCTGCACTGAAGTTCAATGGTGGGGGACATATTAATCACACCATTTTCTGGA CAAACCTGAGCCCTAAGGGTGGTGGAGAACCCAAAGGAGAGTTGCTGGAGGCTATCAAGCGTGACTTTGG GTCTTTTGAGAAGTTTAAGGAGAAGCTGACAGCCGTGTCTGTGGGAGTCCAAGGTTCAGGCTGGGGCTGG CTTGGCTTCAATAAGGAGCAAGGTCGCTTACAGATTGCTGCCTGCTCTAATCAGGACCCATTGCAAGGAA CAACAGGCCTTATTCCGCTGCTGGGGATTGACGTGTGGGAGCACGCTTACTACCTTCAGTATAAAAACGT CAGACCTGACTATCTGAAAGCTATTTGGAATGTAATCAACTGGGAGAATGTTACTGAAAGATACACAGCT TGCAAGAAGTGAAACCTCACTCACGGCCACATTGAGTGCCAGGCTCCGGGCTGGTTTATAGTAGTGTAGA GCATTGCAGCACTATGACTGGGGTGCTGTAGTCTTTATTGATGTCTTTCCACATACCTGATAATTCTATG ATAATTTCTTATTTTAATTAAATCTATTCTTAGGCAACTATTTGAGAACAGCGCATACTCTGTGTGAATT GCTCTTGATTGAACATTTTCGTTAGAGCCTTGAATTGCTTGGATGCTATAGCTGTCATTATAAGACCATC AGAAATATCCCATCTCTGTTTTGGGGGGCCTGTAGGAGAGTCTGTAATCCTGTTCTACTGCAGTTAGAAA AAAAGTTGAGTTGTTCCCCCCTGCCCCCTGAGTTGTTGAATAGTGAAATAGAGAACCAAATAGTTCTCTT TTCTATTAAAAATTGCTATTTTTTGTAAGTAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3581909 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3581909 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7295 same embryo
	EMAGE:7297 same embryo
	EMAGE:7292 same embryo
	EMAGE:7293 same embryo
	EMAGE:7294 same embryo
	EurExpress:euxassay_004393 same experiment
	MGI:4828374 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source