EMAGE mouse gene expression spatial database

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EMAGE:7364

Slco1a5 solute carrier organic anion transporter family, member 1a5 ( MGI:1351865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004472. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004472_01	euxassay_004472_02	euxassay_004472_03	euxassay_004472_04

euxassay_004472_05	euxassay_004472_06	euxassay_004472_07	euxassay_004472_08	euxassay_004472_09

euxassay_004472_10	euxassay_004472_11	euxassay_004472_12	euxassay_004472_13	euxassay_004472_14

euxassay_004472_15	euxassay_004472_16	euxassay_004472_17	euxassay_004472_18	euxassay_004472_19

euxassay_004472_20	euxassay_004472_21	euxassay_004472_22	euxassay_004472_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7364_wholemount_strong.wlz

7364_wholemount_moderate.wlz

7364_wholemount_weak.wlz

7364_wholemount_possible.wlz

7364_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7364_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 19

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20

saccule

weak

regional

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

utricle

weak

regional

weak expression: see section 03 04 05 06 07 08 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6459
Entity Detected:	Slco1a5, solute carrier organic anion transporter family, member 1a5 ( MGI:1351865)
Sequence:	sense strand is shown >T6459 ATTTGCTGCTGTGAATTTCTTTTTCCGTCACCAGCGAGAACACCATTTTCTAAAGCTTCTTCATGTATGT ACTTCTCATTTGGCCAAGTGGTAAGACTGTTTAAAGAATAGTCATTTGGAGACAATCAGAAGAACAACAT GGGAGAAACAGAGAAAAGGATTGCAACCCATGGGGTCAGATGCTTTTCTAAGATCAAGATGTTTCTGTTG GCATTAACATGTGCATATGTATCCAAATCACTCTCAGGAATTTATATGAATTCCATGCTTACACAAATAG AGAGACAATTTGATATCCCCACATCTATAGTTGGACTTATCAATGGGAGCTTTGAGATTGGAAATCTTTT GTTGATTATACTCGTGAGTTACTTTGGAACAAAATTGCACAGACCCATCATGATTGGTATTGGATGTGTG ATTATGGGCCTAGGGTGTTTCTTAATGTCCTTACCTCATTTCCTCATGGGCCGATACGAATATGAAACAA CGATTTCACCTACAAGCAACTTGTCCTCAAACAGCTTCTTGTGTATGGAAAACAGAACACAGACCTTAAA GCCAACGCAAGACCCTGCAGAGTGTGTGAAAGAAATGAAATCATTAATGTGGATATATGTACTGGTAGGA AACATCATACGTGGAATTGGTGAAACTCCCATCATGCCCTTGGGTATTTCCTACATAGAAGATTTTGCCA AATCTGAAAACTCTCCTTTATATATTGGGATTTTAGAATCAGGAAAGATGATTGGCCCGATTGTTGGACT TTTGTTGGGATCCTTCTGTGCAAGAATTTATGTAGACACAGGGTCTGTGAATACAGATGACCTGACCATA ACTCCCACTGATACACGCTGGGTCGGTGCTTGGTGGATTGGCTTTTTGGTCTGTGCAGGAGTGAATATCC TGACCAGCATCCCCTTTTTCTTCTTTCCAAAAACACTCCCAAAGGAAGGATTACAGGATAATGTGGCTAG AACTGAAAATGACAAAGAGGAGAAGCACAGAGAAAAAGCCAAGGAGGAAAACCGAGGAATCACTAAAGAT TTCCTGCCATTCATGAAGAGCCTCTCCTGCAATCCTATTTACATGCTTCTCATCCTGACAAGCGTGCTCC AGATAAACGCATTTATCAATATGTTTACCTTCTTGCCCAAATACCTGGAGCAGCAGTATGGAAAATCCAC TTCAGAGGTCGTCCTCCTCATAGGTGTTTGTAACTTACCTCCAATCTGCATTGGATATTTACTTATTGGC TTTATTATGAAGAAGTTCAGGATTACTGTCAAGAAGGCTGCATACATGGCATTCTGCCTATCCCTGTTTG AGTACCTTCTGTCTTACTTTCACTTTATGATAAGCTGTGATAATTTCCAAGTTGCTGGCTTAACTACCTC TTATGAAGGAGTTCAGCACCCTCTATATGTGGAGAACAAGGTCCTTGCTGACTGCAACACAAGGTGTAGC TGCTTAACAAACACATGGGATCCAGTGTGTGGAGACAATGGCCTATCATACATGTCAGCCTGCCTTGCGG GCTGTGAGAAGTCTGTTGGAATGGGAACCCACATGGTGTTTCAAAATTGCAGCTGCATTCAGTCATCAGG AAACTCATCTGCAGTCCTGGGGCTGTGTAAAAAAGGCCCTGAGTGTGCTAACAAGCTGCAGTACTTTTTA ATCATGTCGGTAATTGGCAGTTTCATCTATTCGATCACAGCCATACCTGGGTACATGGTTCTTCTGAGGT GTATCAAGTCTGAAGAGAAGTCACTTGGAATTGGATTACACGCATTTTGCACAAGAATATTTGCTGGCAT CCCTGCACCCATTTACTTTGGAGCTTTGATAGACAGAACCTGCTTACATTGGGGAACTCTGAAATGTGGT GAGCCAGGAGCATGTAGGATATACAATATAAATAACTTCAGGCGCATTTATCTAGTGTTGCCTGCAGCAC TAAGAGGATCAAGCTATCTCCCTGCATTCTTCATTCTAATACTTATGAGGAAATTCCAGCTCCCTGGGGA AATGTACTCTTCAGAAACTGAACTTGCAGACATGAAGCAAACAGTGAAGAAAAGCGAGTGCACAGATGTG CACGGAATTCCTAAGGTCGAGAATGATGGAGAACTGAAAACTAAGCTGTAATGAATGGCAATGGAAACCT GCTGGCCTGTGCAAGGCTCTGAACAGAATGCACATTGCACTTCCTTTGAATCATGAGAGGTACGAGAGAA CTCCTTTCCTAAAATAAATCAGGGCTGGACACAGTGTAAGATATTCTCAAAAGTCACCGTCTCATCCTCA GGAGGCCTCTGGTGCTCACTCATTCTAGTCACATATCCCAGAGCACAGTGCTACTTTCAAAATCATCTTA AAGTAAAACCTTTGAAGAGGGGCCACCTAAATGGCCCAATGGAAAGGTGTGCTTTCTACCAAGGCTGACA AGGTGTGTTTGATCCTTGGGACCAACATGGCAGAAGGACAGAACCAACCTCGCTTTCAGGTTGTCCTTTT CTCTCTACTCCCGCACTGTGGTGTGCATATCCCACCCCAAACAAACAAACAAATAAAAAAATTTAGAAAC TAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3586426 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3586426 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7362 same embryo
	EMAGE:7361 same embryo
	EMAGE:7360 same embryo
	EMAGE:7359 same embryo
	EMAGE:7363 same embryo
	EurExpress:euxassay_004472 same experiment
	MGI:4828294 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source