EMAGE mouse gene expression spatial database

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EMAGE:7426

Ppm1f protein phosphatase 1F (PP2C domain containing) ( MGI:1918464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004441. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004441_01	euxassay_004441_02	euxassay_004441_03	euxassay_004441_04

euxassay_004441_05	euxassay_004441_06	euxassay_004441_07	euxassay_004441_08	euxassay_004441_09

euxassay_004441_10	euxassay_004441_11	euxassay_004441_12	euxassay_004441_13	euxassay_004441_14

euxassay_004441_15	euxassay_004441_16	euxassay_004441_17	euxassay_004441_18	euxassay_004441_19

euxassay_004441_20	euxassay_004441_21	euxassay_004441_22	euxassay_004441_23	euxassay_004441_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7426_wholemount_strong.wlz

7426_wholemount_moderate.wlz

7426_wholemount_weak.wlz

7426_wholemount_possible.wlz

7426_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7426_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

moderate

regional

moderate expression: see section 01 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7557
Entity Detected:	Ppm1f, protein phosphatase 1F (PP2C domain containing) ( MGI:1918464)
Sequence:	sense strand is shown >T7557 GGCAGAGTGAAGACTTGACTGGAGACTGTCTCACCCCAGCCTAGCTGCAAACCCCCGGAATGCACTGGGC ATGGCCTCTGGAGCCGCACAGAACAGCAGCCAGATGGCCTGTGACAGTGAGATCCCAGGCTTCCTGGACG CATTCCTCCAAGACTTCCCAGCCCCACTGAGCCTGGAGAGCCCTTTGCCATGGAAGGTCCCAGGGACAGT CCTGAGCCAGGAAGAAGTGGAGGCTGAGCTGATTGAGCTGGCATTGGGCTTCCTGGGCAGCAGGAACGCT CCCCCATCGTTTGCTGTGGCTGTGACCCACGAGGCAATTTCCCAGCTGCTGCAGACAGACCTTTCTGAAT TTAAGAGGTTGCCTGAACAAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAGAAAGCCCTCGTGACCTTACT AGATGCCAAAGGCCTGGCACGGAGCTTCTTCAACTGCCTTTGGAAAGTATGTAGCCAGTGGCAGAAGCAG GTGCCGTTGACTGCCCAGGCCCCTCAGTGGCAGTGGCTGGTCTCTATCCATGCCATCCGGAACACACGAC GCAAGATGGAGGACCGACACGTGTCCCTTCCTGCTTTCAACCATCTCTTCGGCTTGTCTGACTCTGTGCA CCGTGCCTACTTTGCTGTGTTTGATGGTCATGGAGGCGTGGATGCAGCAAGGTATGCCTCTGTCCACGTG CATACCAATGCTTCTCACCAGCCAGAGCTGCGCACAAACCCTGCAGCAGCTCTCAAAGAAGCCTTCCGGC TCACCGATGAGATGTTTCTCCAGAAAGCCAAGCGAGAGCGACTGCAGAGCGGTACCACGGGTGTGTGTGC ACTCATCGCGGGAGCAGCCCTGCATGTTGCCTGGCTTGGAGACTCCCAGGTCATCCTGGTACAGCAAGGA CGGGTAGTGAAGCTGATGGAGCCGCACAAACCGGAGCGACAGGATGAGAAAGCACGAATTGAAGCCCTGG GTGGCTTTGTTTCTCTCATGGACTGTTGGAGAGTCAACGGGACCCTGGCAGTGTCTAGAGCCATTGGGGA TGTCTTCCAGAAGCCCTATGTGTCTGGGGAGGCCGATGCAGCATCCAGAGAGCTAACGGGCTCAGAGGAC TACCTGTTGCTTGCTTGTGATGGTTTCTTCGATGTTGTCCCCCACCATGAAGTCACGGGTCTCGTCCATG GCCACTTGCTCAGGCACAAGGGCAATGGGATGCGTATTGCTGAGGAGCTGGTGGCTGTGGCCCGTGACCG GGGCTCCCATGACAACATTACAGTCATGGTGGTCTTCCTTAGGGAGCCCCTGGAGCTGCTAGAGGGTGGA GTCCAGGGGACAGGGGATGCCCAGGCAGATGTAGGAAGCCAGGACTTGTCCACTGGCCTCTCAGAACTTG AGATCAGTAATACCTCACAGAGAAGCTAAGTGGTCCAGGCCTCCAGGCCCCATCCGGACTCTCCCTCTGC CCTCGTGATCCTCCCCTTCAAAGACCTTAGATCCAAAAGGTATAGTGGGCAGGGGTGCACACTCTCACAG CATTCCCTTAGCACCCCAGCCCTTCATGTTGCCTACCACAGCCTGTCCTCATGGCTGCAGAACTGCAGTA GGCAGCTAGTGGATCTCAGAAGGAAACATAGGAAAATGGCCTCACCGAAGAGCAGATGGCTGAGGTGATC AGGGCCGGACAGGTCTGGGAACAGAAACCACAGGCACCTGCTGGTAGCCTAACCAAAAACACAGGTCAGG TCTTGGAGGACCCTCACACAGATCCTGGAATGAGATCTGCAGCCAGGTGTCCAGCCCAGGCTTGTGGCTT CTCATTGTACCCAAGGCTGGGAGGGTTTGGTCTGTACTAACACACAAGCTCGCAGTCCTGCTTGACTGCT GGCTTCCCAAAGAGGAGACATTGGTCTTGCTGGGAGGCACAGCAGGAGAGTGACCCACTGCCACTGCACT CTAACTGAGTACTAAGGCCACTAGGGCTTTCTAGACCTCGCTTTCCCCTTGAGCTTCCTGGGGAGGTGAA GTGAGGTGTGTGTGTGTGTGTGTGTCTTTGTGTGCTTAGATTTATTGCAGGGAAAGGTCTAATCCAGAAT CAGTATTCAGGCTTTGTCATGTTGTATCAGTGCCAAGGTGACCCTCAAGGTCATGTAACTTAAGCAAAGC TTAGCATTTATTTTATTCCTGAAAACTTAAGTATTTTACTTTTTTGTGTGTTCGTGGAGACATTTGCAGT ATTAATGATTTTATTTTTCCTAAATCGGGATGGAAACAAACTTTTCCAGGTTATGTTAATAAGCCACTTA AGTGCCTTAAACAGCTTTGGTGTAGATGAGAATTGCTGGGTCCGTCATGGATCCTGATAGGCAGACGTGT TGAGGTTCTCAAAGAGGCTGCATTTGTTGTGGGAAGGCACACTTTCAGCCAGCACTCTTTCTGGGAAAGT CCTTTTGTGCGTCTGGGCACCTCAGAACTTCCTTGAAGTGATGGGTAAAGGCTGTGACTATGGGCACCTG GGGTGACACTGGCCTCCTTGTCCACCTTGGAGTCTGGCTGGTTCCCAGCTGAGAGAACCTTACTGCTCTT GGCTAGAAGATTGACACACTCAATCTCTCTTGAAACTCTCTTAAGTGTGTTTGCAGTTATGTTGATTGAG TTGTTGAAGACAGTGCTTAGGTGAGGAACTTGGGCAAGCCCAGCAGTCCACCATCTTTCTCCTGTGACTT GTGTCCTAACCTGCTAGACTCTGTCCTGACCTGAGCAGCATGAAAGACCTCCACATCCCTAGAGCAGTGA TGGATACAGTCAGGCAAGGAAACCAAGTGAGGGTTGGGGGGGATACCAAGAATAGAAGGACTAGGAGGCC CTCAACAAGGGCCAAGCGAGTCAAGGCTGCTCTCTGCAGACACTGGCAGGTCCAGAGAATAATGGTGGCT TAGCAGAGGCTCAGGGAAGGATCAGTAGGAAGAAGGGTGGCTGTGAGGTAGGTCCCCATGAAGGAGACAT AGTTCACCTGTAGAGTGAAAGCCCTAGCAAGACTTGCAGAGCTTTGTTCATTGCACCTAAGCCCTCTTTG AAAAGAAGCAAGGGAGCTCATACTTATTCCTCTGAGGCAAGTTCACTTCCAGCAGTAGGAGAGCGAGCCA GCCAAATGCAGCTGGGCACCCACCTGTATGCTTGCCATTTACCCATTCCCACAGCAGGGATGGAAGAGCT GGGGCCATGTAGTTGATTCCTTGATCAAGTCTCCTGATAGGAACGTGATCACTCAGATGGCTGCCCACCT AATAGCTAGGTATTGCTTAACTTGAAGGCCTGCTGCCTTCCGGGGGGGGGGGGGGTGTCAGAATCCAGCA TCCTCAGTGCCATCTCAACACAGGGTGTGGTTGTTGGTCCAGCCTGCCTGTAGCAGTGACCTTCATAGAG GGAGATAAATTGATTTTGGTTTTCTCCCATTTTTGCTAACCTGATCATTGCTGGTAAGAGTGGAATTTGG GCCTCCTTGGTGGTCTGGATTCATACTCGCATGTAAAGATGTGCCAAATATCAAGGCCTCAGTGTAAAGG GAAGCCCCTTTCCCAGGCTTGTCTGAGTGCCTCCCCCACCCTTCCCCCTGTCTGGCAATGGTCTGGGACC AACATCAGGCACTGCTCCATCCTGATCTAGTGGCCAAGGAAGACCACATTCTCCTACTGTTGAGGGCTGG CCTTGAGCGAGTTCCTAGGTACAGAGAACTCTTTTTGTCTGGACTGGAGTTGCCAGTACCAAGCCTACAA GACTTGTTGGCCACCTCCTCCTCAGAGTCTCTTCCCAAAAGAATCCCCTAGCCAGGATAATGGGTGGCTT AGTAGAGGCTCAGGGAAGGAACAGGGAGAAGGGTGGCTGTGAGCTCAAGTCCCCCTCTTTGCCACCATCA CATTGTGTGAGCGGAACCAGTCACTGCACCTCTCAGGCCTCAGGGAAAGGCATGGAGGGCATGCATAGCA TTACCAGGAGCTGCCAGAGCACGGGAGCCCATCCTCCACCAGCAGCAGAGAGGGGTTAAGTATTATTAGT GTCTATTCTTAAAATTAAGTGGGCTGGAACAGAGCTGAGTTACAGGCGCCAGTGTCTTACACAGATACAA AAGCTGAAGCCGTCAGCCTGCCTTTGCAGGTACAGCTGCCTCCCAACCAAGGCCTGGAGGCGTTAAGAGG TGGGAGGGCACAAGCAGGCTTTCAGGAGTGTTGAAGCAAGGGATCTGCAGCATAGCTTTGGGCGTCTCCA GAACTCATGGTGAGGGAGGCACCAACACCTAGAGGTCTTAAAAGCCCTGCATTCACCATGATCTGTCTGC CCCTGCCCTTTTGGGTTCTTCTGCATGCAGTGCCCCCCTCTCCTGATGCTAAGTTTTGGCATGGGTGTCC TGGCTACCTCAACTGTATTCTCAAGGCCAGGAGGATGGCTCTGGCCATACTTACGGAAGCCAGGCCAATT TGTGTGAAGCAAGCTGGGCTTCTGGTTTGATTCAGCTTCTGTAGCCTCCAGGGACCATCCTCTGGAGTCT TGTGCTTTCACTGGACTCTTCAGCTTCACCAAACACACCAAGCTTTGCCATTTACATGCCATGTGGGCCT TGCCAAGGGTTATTTCAGGGGTCTTCCTGGGAGTCTTGTTCTAAGACACCGTGTTTCTTTAGTCTTAGGG ACAACTCCCAAGGTGATGAGCTCATAGGACGTCTTCCCATGGGGCCCTGTACTGGTGTCAATAAACCATA TATTCAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241448 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241448 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7425 same embryo
	EMAGE:7424 same embryo
	EMAGE:7429 same embryo
	EMAGE:7428 same embryo
	EMAGE:7427 same embryo
	EurExpress:euxassay_004441 same experiment
	MGI:4827363 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source