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EMAGE:8949

Nedd4 neural precursor cell expressed, developmentally down-regulated 4 ( MGI:97297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949
"Pseudo-wholemount" of euxassay_012911. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012911_01 euxassay_012911_02 euxassay_012911_03 euxassay_012911_04
EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949
euxassay_012911_05 euxassay_012911_06 euxassay_012911_07 euxassay_012911_08 euxassay_012911_09
EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949
euxassay_012911_10 euxassay_012911_11 euxassay_012911_12 euxassay_012911_13 euxassay_012911_14
EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949
euxassay_012911_15 euxassay_012911_16 euxassay_012911_17 euxassay_012911_18 euxassay_012911_19
EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949 EMAGE:8949
euxassay_012911_20 euxassay_012911_21 euxassay_012911_22 euxassay_012911_23 euxassay_012911_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8949Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8949_wholemount_strong.wlz
8949_wholemount_moderate.wlz
8949_wholemount_weak.wlz
8949_wholemount_possible.wlz
8949_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8949_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
body cavity or lining
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
limb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
gland
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
alimentary system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
nervous system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
integumental system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
sensory organ system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
cardiovascular system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
urinary system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
reproductive system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
respiratory system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
tail
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38687
Entity Detected:Nedd4, neural precursor cell expressed, developmentally down-regulated 4 ( MGI:97297)
Sequence:sense strand is shown

>T38687
GAGACAAGTGGAGCAAGCTTTTATTGCAGTGTGTAGTGAGTCTTAGGCCTCCTTTTTGCCTGTGCATCCT
AAGGCTAGAGTCATTATGTGTTAGTGCGTTAGAATTCCTTCAAACACGAAACAAGCTCCACAACGCAGTG
TTTCTAAGCATGCCGTGAAGAACCGAGTTCCATGTATGACTTGCCCTTACCAGCCACTCTTCACGCTTGC
AGTCACGTAGTACATACGTGTTTACGGAGTTCATTATGTTTACAGATTAAGCGAATTTCTGTAGTTGCAT
TTTTATATTTTTAGTATCACGTTAGTATAAAAATTTGTTTAAAATAACCAAAGAGTAGTTCAATGCATAA
TTTGTATGAATTTGTTACCAAGTTTCTTATGCTTTCTAAAAAAATACTGTTTCCTATGAAAATTATGTTT
AATCAAAAGTCAAGAACCCTTGGGGCAAAATGCTACAGGTGGAGTCCCACCATAGTCACCTTTGAGGCAC
AGGAAGAGGTTCCATGCATGAATCTGCACACATGAGCAGTGCTGGCTGCTGTGCACCGGGCCCTTGGGAT
GAAGCCATCAGCACTGACATCCAGCCCCGTCTTCATCCTCAGGCTTAGAAACAAGTGGGATGGTGACTTT
ACAGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 153822. Forward Primer - name:153822_F_cDNA_TC1515129, sequence:GAGACAAGTGGAGCAAGCTTTT; Reverse Primer - name:153822_N_SP6_cDNA_TC1515129, sequence:GACTGTAAAGTCACCATCCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8950 same embryo
 EMAGE:8948 same embryo
 EMAGE:8947 same embryo
 EMAGE:8951 same embryo
 EMAGE:8946 same embryo
 EurExpress:euxassay_012911 same experiment
 MGI:4826655 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS