EMAGE mouse gene expression spatial database

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EMAGE:9524

Nkx2-5 NK2 transcription factor related, locus 5 (Drosophila) ( MGI:97350)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018369. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018369_01	euxassay_018369_02	euxassay_018369_03	euxassay_018369_04

euxassay_018369_05	euxassay_018369_06	euxassay_018369_07	euxassay_018369_08	euxassay_018369_09

euxassay_018369_10	euxassay_018369_11	euxassay_018369_12	euxassay_018369_13	euxassay_018369_14

euxassay_018369_15	euxassay_018369_16	euxassay_018369_17	euxassay_018369_18	euxassay_018369_19

euxassay_018369_20	euxassay_018369_21	euxassay_018369_22	euxassay_018369_23	euxassay_018369_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9524_wholemount_strong.wlz

9524_wholemount_moderate.wlz

9524_wholemount_weak.wlz

9524_wholemount_possible.wlz

9524_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9524_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15 16

corpus striatum

moderate

regional

moderate expression: see section 06 weak expression: see section 07 22 23 24

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 weak expression: see section 03 04

pons mantle layer

moderate

single cell

moderate expression: see section 09 18 weak expression: see section 10 17 19

midbrain mantle layer

moderate

single cell

moderate expression: see section 11 12 15 16 weak expression: see section 06 07 14 17 19

tongue muscle

strong

regional

strong expression: see section 17 moderate expression: see section 12 13 14 15 16 weak expression: see section 18

liver

strong

regional

strong expression: see section 17 18 19 20 21 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50740
Entity Detected:	Nkx2-5, NK2 transcription factor related, locus 5 (Drosophila) ( MGI:97350)
Sequence:	sense strand is shown >T50740 CTACGGCGTGGGTCTCAATGCCTATGGCTACAACGCCTACCCCTACCCCAGCTACGGCGGCGCGGCCTGC AGTCCCGGCTACAGCTGCGCCGCCTACCCCGCTGCGCCCCCCGCCGCGCAGCCCCCCGCCGCCTCCGCCA ACAGCAACTTCGTGAACTTTGGCGTCGGGGACTTGAACACCGTGCAGAGTCCCGGGATGCCGCAGGGCAA TTCGGGCGTCTCCACGCTGCACGGCATCCGAGCCTGGTAGGGAAAGAGCCCGTTTGGGGCGCCCCGGAAC GACTCCCACCTTTAGGAGAAGGGCGATGACTCCGGGGATGGGAAAGCTCCCACTATGCCCTGTCCCTCGG ATTTCACACCCACCCTCGCGCAGGCCTGGGACCTTTCTCCGATCCATCCCACTTTATTGACGTAGCCTGG TGTCTCGGACCTGGCAGAGCCTTCCAGGGTCACGGGCACTTTCGACGGATTCCACACTAGGACCGGGAGC CTGGGCCGGGCGCCCGGGCCCTGGTTGTCTTGCCGTCGCCCACCCACCCGTATTTATGTTTTTACCTGTT GTAAGAAATGAGAACCCTCTTCCCATTAAAGTGAGTGCGTAACG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTACGGCGTGGGTCTCAAT; Reverse Primer - name:unspecified, sequence:CGTTACGCACTCACTTTAATGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826724 same experiment
	EurExpress:euxassay_018369 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source