| Technique |
Advantages of OPT |
Disadvantages of OPT |
confocal
|
- can image bigger specimen
- can image non fluorescent specimen, such as BCIP/NBT stained in-situs, or LacZ staining
- cheaper
|
- cannot image live specimen
- lower resolution
|
OCT
|
- can image bigger specimen
- can image commonly-used coloured or fluorescent dyes
- cheaper
|
- cannot image live specimen
|
mMRI
|
- can image smaller specimen with higher resolution
- faster
- much cheaper
|
- cannot image live specimen
- lower contrast for unstained tissue
|
Serial sections
|
- faster
- retains original 3D shape
|
|
