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EMAGE:6141

Wnt3a wingless-related MMTV integration site 3A ( MGI:98956)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:6141 EMAGE:6141 EMAGE:6141 EMAGE:6141 EMAGE:6141
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:6141Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
6141_voxel_strong_3D_1.wlz
6141_voxel_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6141_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt3a probeA
Entity Detected:Wnt3a, wingless-related MMTV integration site 3A ( MGI:98956)
Sequence:sense strand is shown

>Wnt3a probeA
AGAGCCAGGGTTGTTTCAGGACCCATCTGATTCCCCAGAGCCTGCTGTTGAGGCAATGGTCACCAGATCC
GTTGGCCACCACCCTGTCCCGAGCTTCTCTAGTGTCTGTCTGGCCTGGAAGTGAGGTGCTACATACAGCC
CATCTGCCACAAGAGCTTCCTGATTGGTACCACTGTGAACCGTCCCTCCCCCTCCAGACAGGGGAGGGGA
TGTGGCCATACAGGAGTGTGCCCGGAGAGCGCGGAAAGAGGAAGAGAGGCTGCACACGCGTGGTGACTGA
CTGTCTTCTGCCTGGAACTTTGCGTTCGCGCTTGTAACTTTATTTTCAATGCTGCTATATCCACCCACCA
CTGGATTTAGACAAAAG
nt 2310 - nt 2676 of NM_009522.1
Notes:The Wnt3a probe template used in this study was generated by RT-PCR from 9.5 embryo mRNA using the following primers: forward: 5'-AGAGCCAGGGTTGTTTCAGGA-3'; reverse: 5'-CTTTTGTCTAAATCCAGTGGTGGG-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). Probe was transcribed from the entire insert.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE