EMAGE mouse gene expression spatial database

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EMAGE:11872

Lama3 laminin, alpha 3 ( MGI:99909)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011044. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011044_01	euxassay_011044_02	euxassay_011044_03	euxassay_011044_04

euxassay_011044_05	euxassay_011044_06	euxassay_011044_07	euxassay_011044_08	euxassay_011044_09

euxassay_011044_10	euxassay_011044_11	euxassay_011044_12	euxassay_011044_13	euxassay_011044_14

euxassay_011044_15	euxassay_011044_16	euxassay_011044_17	euxassay_011044_18	euxassay_011044_19

euxassay_011044_20	euxassay_011044_21	euxassay_011044_22	euxassay_011044_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11872_wholemount_strong.wlz

11872_wholemount_moderate.wlz

11872_wholemount_weak.wlz

11872_wholemount_possible.wlz

11872_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11872_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

weak

regional

weak expression: see section 06 07 08 09 21 22 23

metencephalon floor plate

moderate

regional

moderate expression: see section 12

spinal cord floor plate

weak

regional

weak expression: see section 13 14 15

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 04 05 22 23

naris

weak

regional

weak expression: see section 14 15 17 18 19

nasal septum

weak

regional

weak expression: see section 14 15

esophagus

weak

regional

weak expression: see section 13 14

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

oral epithelium

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

palatal shelf epithelium

weak

regional

weak expression: see section 11 12 13 15 16 17

upper jaw incisor

weak

regional

weak expression: see section 13 14 17 18

urethra of male

weak

regional

weak expression: see section 15

lung

strong

regional

strong expression: see section 04 05 06 07 09 10 11 13 15 17 18 19 20 21 22 23 moderate expression: see section 08 12 14 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36577
Entity Detected:	Lama3, laminin, alpha 3 ( MGI:99909)
Sequence:	sense strand is shown >T36577 TCTGAGAAGAAACCAAAGGCTCGCTAGCTTCTCTAATGCCCAGCAGTCGCTCAGCATGGGAGGCGGTTAT TTCGAGGGTTGTATCAGCAACGTTTTTGTCCAAAGGATGTCACAGAGTCCAGAAGTCCTGGATATGGCCA GCAAGTCTACTAAGAGGGATGCATTCCTAGGAGGCTGCAGTTTAAACAAGCCACCTTTTCTTATGTTGTT TAAGAGTCCCAAGGGATTTAACAAGGCCCGGAGTTTCAATGTCAATCAGCTGTTGCAAGATGCACCTCAG GCTGCAAGGAGCATAGAGGCTTGGCAAGATGGGAAGTCCTGCCTACCACCTCTGAACACCAAGGCCACTC ACAGAGCCCTGCAGTTTGGGGACAGTCCTACCAGCCACTTGCTATTCAAGCTTCCCCAGGAGCTGCTGAA ACCCAGGTTACAGTTTTCTTTGGACATACAGACAACTTCCTCCAGAGGGCTAGTGTTTCACACAGGCACC AGGGACTCCTTTGTGGCTCTCTATCTCTCAGAAGGCCATGTCATCTTTGCCTTGGGGGCAGGAGGGAAGA AACTGAGACTCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85681. Forward Primer - name:085681_F_cDNA_Lama3, sequence:TCTGAGAAGAAACCAAAGGCTC; Reverse Primer - name:085681_N_SP6_cDNA_Lama3, sequence:TGAGTCTCAGTTTCTTCCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11870 same embryo
	EMAGE:11869 same embryo
	EMAGE:11868 same embryo
	EMAGE:11871 same embryo
	EMAGE:11873 same embryo
	EurExpress:euxassay_011044 same experiment
	MGI:4825858 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source