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EMAGE:154

Fgf8 fibroblast growth factor 8 ( MGI:99604)
TS15
in situ hybridisation

Data Images
EMAGE:154

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:154Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
154_wholemount_strong_3D_1.wlz
154_wholemount_moderate_3D_1.wlz
154_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:154_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fgf8 cloneA
Entity Detected:Fgf8, fibroblast growth factor 8 ( MGI:99604)
Notes:The Fgf8 probe used here is 400nt in length. The template clone is 800nt in length and was a gift from Phil Crossley and Gail Martin, UCSF. PstI was used to linearise the template clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kirsten Steiner, Kenneth Soo, Patrick Tam
Principal investigator:Patrick Tam, Children's Medical Research Institute, Embryology Unit, CMRI, Locked Bag 23 Wentworthville, Sydney, Australia 2145
Submitted by:Kirsten Steiner, Children's Medical Research Institute, Embryology Unit, CMRI, Locked Bag 23 Wentworthville, Sydney, Australia 2145
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE