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EMAGE:3394

Fgf8 fibroblast growth factor 8 ( MGI:99604)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3394
Figure 8D of Boyl et al., 2001 [PMID:11532921] . Copyright: This image is from Boyl PP, Development 2001;128():2989-3000, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3394EMAGE:3394Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3394_voxel_strong_3D_1.wlz
3394_voxel_possible_3D_1.wlz
3394_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3394_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalExpression is at the midbrain/hindbrain junction and in the future isthmus.
midbrain
detected detected
regionalExpression is at the midbrain/hindbrain junction.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1098117
Entity Detected:Fgf8, fibroblast growth factor 8 ( MGI:99604)
Notes:The Fgf8 probe used in this study by Boyl et al., 2001 [PMID:11532921] was originally described by Acampora et al., 1997 [PMID:9342056] as a "712 bp PCR product corresponding to the AIGF1 variant (Tanaka et al., 1992 [PMID:1409588] ) and containing the coding region from amino acid 1 (Crossley et al., 1996 [PMID:8598907] ) plus 103 bp of 3' UT". Editor's note: Editor's Note: Tanaka et al, describe two clones pSC17 and pSC15. It is unclear from the text which of these is the "variant" described by Acampora et al.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Boyl PP; Signore M; Acampora D; Martinez-Barbera JP; Ilengo C; Annino A; Corte G; Simeone A, 2001 [PMID:11532921] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11532921] Boyl PP, Signore M, Acampora D, Martinez-Barbera JP, Ilengo C, Annino A, Corte G, Simeone A 2001 Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR. Development (128):2989-3000
 [ PMID:9342056] Acampora D, Avantaggiato V, Tuorto F, Simeone A 1997 Genetic control of brain morphogenesis through Otx gene dosage requirement. Development (124):3639-50
 [ doi:10.1073/pnas.89.19.8928] [ PMID:1409588] Tanaka A, Miyamoto K, Minamino N, Takeda M, Sato B, Matsuo H, Matsumoto K 1992 Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells. Proc Natl Acad Sci U S A (89):8928-32
 [ doi:10.1038/380066a0] [ PMID:8598907] Crossley PH, Martinez S, Martin GR 1996 Midbrain development induced by FGF8 in the chick embryo. Nature (380):66-8
Links:MGI:2150158 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI