Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3900

Fgf8 fibroblast growth factor 8 ( MGI:99604)
TS14 (9.2 dpc)
in situ hybridisation

Data Images
EMAGE:3900
Figure 2G. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00056-5] Gene Expr Patterns 3: 497-501, Acampora D; Annino A; Puelles E; Alfano I; Tuorto F; Simeone A, OTX1 compensates for OTX2 requirement in regionalisation of anterior neuroectoderm. Copyright 2003.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3900Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3900_wholemount_strong_3D_1.wlz
3900_wholemount_moderate_3D_1.wlz
3900_wholemount_weak_3D_1.wlz
3900_wholemount_possible_3D_1.wlz
3900_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3900_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future hindbrain
detected detected
regionalExpression is at the midbrain-hindbrain boundary.
future midbrain
detected detected
regionalExpression is at the midbrain-hindbrain boundary.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1098117
Entity Detected:Fgf8, fibroblast growth factor 8 ( MGI:99604)
Notes:The Fgf8 probe used in this study by Acampora et al., 2003 [PMID:12915318] is indicated as that described by Acampora et al, 1998 [PMID:9811592] , who is turn refer to Acampora et al., 1997 [PMID:9342056] i.e. a "712 bp PCR product corresponding to the AIGF1 variant (Tanaka et al., 1992 [PMID:1409588] ) and containing the coding region from amino acid 1 (Crossley et al., 1996 [PMID:8598907] ) plus 103 bp of 3' UT". Editor's Note: Tanaka et al, describe two clones pSC17 and pSC15. It is unclear from the text which of these is the "variant" described by Acampora et al., 1997.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.2 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Acampora D; Annino A; Puelles E; Alfano I; Tuorto F; Simeone A, 2003 [PMID:12915318] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00056-5] [ PMID:12915318] Acampora D, Annino A, Puelles E, Alfano I, Tuorto F, Simeone A 2003 OTX1 compensates for OTX2 requirement in regionalisation of anterior neuroectoderm. Gene Expr Patterns (3):497-501
 [ PMID:9342056] Acampora D, Avantaggiato V, Tuorto F, Simeone A 1997 Genetic control of brain morphogenesis through Otx gene dosage requirement. Development (124):3639-50
 [ doi:10.1073/pnas.89.19.8928] [ PMID:1409588] Tanaka A, Miyamoto K, Minamino N, Takeda M, Sato B, Matsuo H, Matsumoto K 1992 Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells. Proc Natl Acad Sci U S A (89):8928-32
 [ doi:10.1038/380066a0] [ PMID:8598907] Crossley PH, Martinez S, Martin GR 1996 Midbrain development induced by FGF8 in the chick embryo. Nature (380):66-8
 [ PMID:9811592] Acampora D, Avantaggiato V, Tuorto F, Briata P, Corte G, Simeone A 1998 Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation. Development (125):5091-104
Links:MGI:3045233 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI