Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5296

Fgf8 fibroblast growth factor 8 ( MGI:99604)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5296 EMAGE:5296 EMAGE:5296 EMAGE:5296 EMAGE:5296
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5296 EMAGE:5296 EMAGE:5296
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
handplate apical ectodermal ridge
strong strong
footplate apical ectodermal ridge
strong strong
latero-nasal process
moderate moderate
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fgf8_probeC
Entity Detected:Fgf8, fibroblast growth factor 8 ( MGI:99604)
Sequence:sense strand is shown

>Fgf8_probeC
CATGTGAGGGAGCAGAGCCTGGTGACGGATCAGCTCAGCCGCCGCCTCATCCGGACCTACCAGCTCTACA
GCCGCACCAGCGGGAAGCACGTGCAGGTCCTGGCCAACAAGCGCATCAACGCCATGGCAGAAGACGGAGA
CCCCTTCGCGAAGCTCATTGTGGAGACCGATACTTTTGGAAGCAGAGTCCGAGTTCGCGGCGCAGAGACA
GGTCTCTACATCTGCATGAACAAGAAGGGGAAGCTAATTGCCAAGAGCAACGGCAAAGGCAAGGACTGCG
TATTCACAGAGATCGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACAT
GGCCTTTACCCGCAAGGGCCGGCCCCGCAAGGGCTCCAAGACGCGCCAGCATCAGCGCGAGGTGCACTTC
ATGAAGCGCCTGCCGCGGGGCCACCACACCACCGAGCAGAGCCTGCGCTTCGAGTTCCTCAACTACCCGC
CCTTCACGCGCAGCCTGCGCGGCAGCCAGAGGACTTGGGCCCCGGAG
nt 461 - nt 997 of NM_010205.1
Notes:Probe template cloned into pBluescript to generate pBluescript/AIGF. Antisense probe was generated by cutting with HindIII and transcribing from T3 promoter. Template clone available upon request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1016/S0960-9822(95)00157-6] [ PMID:7583127] 1995 A role for FGF-8 in the initiation and maintenance of vertebrate limb bud outgrowth. Current Biology (5):797-806
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE