EMAGE mouse gene expression spatial database

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EMAGE:5298

Fgf8 fibroblast growth factor 8 ( MGI:99604)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5298_wholemount__moderate.wlz

5298_wholemount__possible.wlz

5298_wholemount__notDetected.wlz

5298_wholemount__strong.wlz

5298_wholemount__weak.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5298_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

1st branchial arch maxillary-mandibular groove

strong

regional

1st branchial arch maxillary component

strong

regional

1st branchial arch mandibular component

strong

regional

forelimb bud

strong

regional

nasal process

strong

regional

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Fgf8_probeC
Entity Detected:	Fgf8, fibroblast growth factor 8 ( MGI:99604)
Sequence:	sense strand is shown >Fgf8_probeC CATGTGAGGGAGCAGAGCCTGGTGACGGATCAGCTCAGCCGCCGCCTCATCCGGACCTACCAGCTCTACA GCCGCACCAGCGGGAAGCACGTGCAGGTCCTGGCCAACAAGCGCATCAACGCCATGGCAGAAGACGGAGA CCCCTTCGCGAAGCTCATTGTGGAGACCGATACTTTTGGAAGCAGAGTCCGAGTTCGCGGCGCAGAGACA GGTCTCTACATCTGCATGAACAAGAAGGGGAAGCTAATTGCCAAGAGCAACGGCAAAGGCAAGGACTGCG TATTCACAGAGATCGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACAT GGCCTTTACCCGCAAGGGCCGGCCCCGCAAGGGCTCCAAGACGCGCCAGCATCAGCGCGAGGTGCACTTC ATGAAGCGCCTGCCGCGGGGCCACCACACCACCGAGCAGAGCCTGCGCTTCGAGTTCCTCAACTACCCGC CCTTCACGCGCAGCCTGCGCGGCAGCCAGAGGACTTGGGCCCCGGAG
	nt 461 - nt 997 of NM_010205.1
Notes:	Probe template cloned into pBluescript to generate pBluescript/AIGF. Antisense probe was generated by cutting with HindIII and transcribing from T3 promoter. Template clone available upon request.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Bozena Palinkasova, Mike Dixon
Principal investigator:	Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:	Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:	screen
References:	[ doi:10.1016/S0960-9822(95)00157-6] [ PMID:7583127] 1995 A role for FGF-8 in the initiation and maintenance of vertebrate limb bud outgrowth. Current Biology (5):797-806
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source