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EMAGE:71

Fgf8 fibroblast growth factor 8 ( MGI:99604)
TS15 (9.7 dpc)
in situ hybridisation

Data Images
EMAGE:71
Figure 4C of Martinez-Barbera et al., 2001 [PMID:11731459] . Copyright: This image is from Development and is displayed with the permission of the Company of Biololsits Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:71Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
71_wholemount_strong_3D_1.wlz
71_wholemount_moderate_3D_1.wlz
71_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:71_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future hindbrain
detected detected
regionalExpression is on the metencephalic side of the midbrain-hindbrain boundary.
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1098117
Entity Detected:Fgf8, fibroblast growth factor 8 ( MGI:99604)
Notes:Probe used in this study by Martinez-Barbera et al., 2001 [PMID:11731459] is indicated as being originally described by Acampora et al., 1997 [PMID:9342056] ie. a "712 bp PCR product corresponding to the AIGF1 variant (Tanaka et al., 1992 [PMID:1409588] ) and containing the coding region from amino acid 1 (Crossley et al., 1996 [PMID:8598907] ) plus 103 bp of 3' UT". Editor's Note: Tanaka et al, describe two clones pSC17 and pSC15. It is unclear from the text which of these is the "variant" described by Acampora et al.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x DBA/2
Age:9.7 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:undefined
General Information
Authors:Martinez-Barbera et al., 2001 [PMID:11731459] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11731459] Martinez-Barbera JP, Signore M, Boyl PP, Puelles E, Acampora D, Gogoi R, Schubert F, Lumsden A, Simeone A 2001 Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development (128):4789-800
 [ doi:10.1073/pnas.89.19.8928] [ PMID:1409588] Tanaka A, Miyamoto K, Minamino N, Takeda M, Sato B, Matsuo H, Matsumoto K 1992 Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells. Proc Natl Acad Sci U S A (89):8928-32
 [ PMID:9342056] Acampora D, Avantaggiato V, Tuorto F, Simeone A 1997 Genetic control of brain morphogenesis through Otx gene dosage requirement. Development (124):3639-50
 [ doi:10.1038/380066a0] [ PMID:8598907] Crossley PH, Martinez S, Martin GR 1996 Midbrain development induced by FGF8 in the chick embryo. Nature (380):66-8
Links:MGI:2155564 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI