EMAGE mouse gene expression spatial database

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EMAGE:10000

1110008P14Rik RIKEN cDNA 1110008P14 gene ( MGI:1920987)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000206_01	euxassay_000206_02	euxassay_000206_03	euxassay_000206_04	euxassay_000206_05

euxassay_000206_06	euxassay_000206_07	euxassay_000206_08	euxassay_000206_09	euxassay_000206_10

euxassay_000206_11	euxassay_000206_12	euxassay_000206_13	euxassay_000206_14	euxassay_000206_15

euxassay_000206_16	euxassay_000206_17	euxassay_000206_18	euxassay_000206_19	euxassay_000206_20

euxassay_000206_21	euxassay_000206_22	euxassay_000206_23	euxassay_000206_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10000_wholemount_strong.wlz

10000_wholemount_moderate.wlz

10000_wholemount_weak.wlz

10000_wholemount_possible.wlz

10000_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10000_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 07 08 09 13 14 16 17 18

pons mantle layer

strong

single cell

strong expression: see section 07 18

facial vii ganglion

strong

single cell

strong expression: see section 05 06 21

glossopharyngeal ix ganglion

strong

single cell

strong expression: see section 08 18 20

trigeminal v ganglion

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

strong

single cell

strong expression: see section 06 07 09 19 20

ventral grey horn

strong

single cell

strong expression: see section 10 11 14 15 16 18

dorsal root ganglion

strong

single cell

strong expression: see section 06 07 08 09 11 15 16 17 18 19 20 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T307
Entity Detected:	1110008P14Rik, RIKEN cDNA 1110008P14 gene ( MGI:1920987)
Sequence:	sense strand is shown >T307 CGCTTCCGGCCCGGTGCTCAACCAGCTGCTCGCACCTTCCGCCCAGCGCCGGCTTTCCGCGCGACGATCG CCAAGTTCCATCTTCGCGTGGCCTCTGCGGCGCCCGAGCCCAATGTCGGGCCCCAACGGGGACCTAGGCA TGCCGGTGGATGCGGGCANGGAAGGAGAGAATGACAGCTTCGGGGAAGCAGAGTATGCTGCCATCAACTC CATGTTGGATCAGATCAACTCTTGTCTGGACCACCTGGAGGAGAAGAATGACCACCTCCATGCCCGCCTC CAGGAGCTGCTGGAGTCGAACCGGCAGACGCGCCTGGAATTCCAGCAACAGCTTGGGGAGGCCCCTGGCG ATGCCAGCCCCTAGGTGCCGGGAGCCCCCATCCAGGCCCTACCCTCACCTCTCTAGGCCATGTTCTGGCC TGGGTAGATACTACTTGGCTTAGACACCATCTCGGGTACTGGCCTCCAGATCCTAGTGGGTCTACCAGCC T
Notes:	The probe template was PCR amplified from IMAGE:2812475 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2812475 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10003 same embryo
	EMAGE:10002 same embryo
	EMAGE:10004 same embryo
	EMAGE:10001 same embryo
	EurExpress:euxassay_000206 same experiment
	MGI:4822590 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source