EMAGE mouse gene expression spatial database

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EMAGE:10003

Vgll4 vestigial like 4 (Drosophila) ( MGI:2652840)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000238_01	euxassay_000238_02	euxassay_000238_03	euxassay_000238_04	euxassay_000238_05

euxassay_000238_06	euxassay_000238_07	euxassay_000238_08	euxassay_000238_09	euxassay_000238_10

euxassay_000238_11	euxassay_000238_12	euxassay_000238_13	euxassay_000238_14	euxassay_000238_15

euxassay_000238_16	euxassay_000238_17	euxassay_000238_18	euxassay_000238_19	euxassay_000238_20

euxassay_000238_21	euxassay_000238_22	euxassay_000238_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10003_wholemount_strong.wlz

10003_wholemount_moderate.wlz

10003_wholemount_weak.wlz

10003_wholemount_possible.wlz

10003_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10003_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 17 18 19

vibrissa

strong

regional

strong expression: see section 04 moderate expression: see section 06 07 08 21 22 23

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

lower jaw molar

moderate

regional

moderate expression: see section 07 19

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16 17

upper jaw molar

moderate

regional

moderate expression: see section 07 08 19

lung

moderate

spotted

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T294
Entity Detected:	Vgll4, vestigial like 4 (Drosophila) ( MGI:2652840)
Sequence:	sense strand is shown >T294 GCTGGATGTGTTGTCCAGGGCAGCCTCTCTGGTGCATGCAGATGATGAAAAACGTGAAGCTTCCCTCAGG GGAGAACCCAGGATGCAGACCCTCCCGGTGGCCTCTGCGCTCAGCAGTCACCGCACAGGCCCGCCTCCCA TCAGCCCCAGCAAGAGGAAATTCAGCATGGAGCCCGGGGACAAGGACTTGGACTGTGAAAACGACCACGT CTCCAAGATGAGCCGCATCTTCAGCCCCCATTTGAATAAGACTGTCAACGGAGACTGCCGCAGAGATCCC CGGGAGCGGAGCCGCAGTCCCATTGAACGGGCTGCAGCCCCCGCCGTGAGCTTGCATGGTGGCCACCTGT ACGCATCTCTCCCCAGCCTCATGGAGCAGCCTCTTGCCCTGACTAAGAACAGCAGTGACACAGGCAGGTC AGCTGTGGAGCGGCAGCAGAACCGGCCCTCTGTGATTACCTGTGCATCCGCGGGTGCCCGCAACTGCAAC CTCTCTCACTGTCCCATCGCAC
Notes:	The probe template was PCR amplified from IMAGE:2811212 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2811212 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10000 same embryo
	EMAGE:10002 same embryo
	EMAGE:10004 same embryo
	EMAGE:10001 same embryo
	EurExpress:euxassay_000238 same experiment
	MGI:4829158 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source