EMAGE mouse gene expression spatial database

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EMAGE:10005

Slc7a3 solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 ( MGI:1100521)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019694. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019694_01	euxassay_019694_02	euxassay_019694_03	euxassay_019694_04

euxassay_019694_05	euxassay_019694_06	euxassay_019694_07	euxassay_019694_08	euxassay_019694_09

euxassay_019694_10	euxassay_019694_11	euxassay_019694_12	euxassay_019694_13	euxassay_019694_14

euxassay_019694_15	euxassay_019694_16	euxassay_019694_17	euxassay_019694_18	euxassay_019694_19

euxassay_019694_20	euxassay_019694_21	euxassay_019694_22	euxassay_019694_23	euxassay_019694_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10005_wholemount_strong.wlz

10005_wholemount_moderate.wlz

10005_wholemount_weak.wlz

10005_wholemount_possible.wlz

10005_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10005_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

spotted

moderate expression: see section 10 11 12 13 14 15 16

hindlimb digit 2 phalanx

weak

regional

weak expression: see section 04

hindlimb digit 3 phalanx

weak

regional

weak expression: see section 04

hindlimb digit 4 phalanx

weak

regional

weak expression: see section 04 05 06

hindlimb digit 5 phalanx

weak

regional

weak expression: see section 07

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 14 15 16

pons ventricular layer

weak

regional

weak expression: see section 10 14 15 16 17

midbrain ventricular layer

weak

regional

weak expression: see section 12 14 15 16

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 15 16

urethra of male

weak

regional

weak expression: see section 15 16

lung

moderate

regional

moderate expression: see section 05 08 09 10 11 weak expression: see section 04 06 07 12 13 15 16 17 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56015
Entity Detected:	Slc7a3, solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 ( MGI:1100521)
Sequence:	sense strand is shown >T56015 ATGCCTTCGTTGGTTTTGACTGTATTGCAACCACCGGAGAAGAGGCTCAGAATCCCCAGCGCTCCATCCC CATGGGTATTGTGATCTCGATGTTCATCTGCTTTTTGGCTTACTTTGGTGTGTCTTCAGCACTTACCCTC ATGATGCCTTACTACAAGCTTCATCCTGAGAGCCCTCTGCCTGAAGCATTTTCCTATGTTGGCTGGGAGC CTGCCCGCTACTTGGTGGCTATTGGCTCCCTCTGTGCACTTTCTACTAGCCTTTTGGGCTCTATGTTTCC TATGCCTCGGGTGATGTATTCAATGGCAGAAGATGGCCTCCTGTTCCGTGTCCTTGCTAAGGTTCACAGT GTCACACATATACCCATTGTGGCCACCTTGGTATCTGGTGTTATTGCAGCATTCATGGCATTCCTCTTTG AACTCACTGACCTCGTGGACCTCATGTCAATCGGGACTCTGCTTGCTCACTCCCTGGTGTCCATTTGCGT TCTCATCCTCAGATATCAGCCTGATCAGGAAATGAAGAGTGTTGAAGAGGAAATGGAATTGCAAGAGGAG ACACTTGAAGCAGAGAAGCTGACTGTCCAGGCTCTATTTTGTCCTGTCAACTCCATCCCCACACTCCTTT CTGGCCGAGTTGTCTATGTTTGCTCCTCACTGCTTGCCGTACTACTAACTGTTCTTTGCCTGGTGCTGAC CTGGTGGACAACTCCACTTCGTTCTGGAGACCCAGTGTGGGTCACAGTGGTTGTGCTGATCCTGGGGCTC ATTCTTGCAATTTCTGGGGTCATCTGGAGACAGCCACAGAACCGCACTCCCCTTCACTTTAAGGTGCCTG CTGTTCCTCTACTCCCACTGGTGAGCATCTTTGTGAATGTTTACCTGATGATGCAAATGACAGCTGGCAC TTGGGCCCGATTTGGGATCTGGATGCTGATTGGATTTGCTATCTACTTTGGCTATGGGATCCAACACAGT ATGAAAGAAGTTAAGAATCATCAAACTCTACCCAAAACAAGGGCCCAAACTA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATGCCTTCGTTGGTTTTGAC; Reverse Primer - name:unspecified, sequence:TAGTTTGGGCCCTTGTTTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828276 same experiment
	EurExpress:euxassay_019694 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source